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AB244925

Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector)

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Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (ab244925) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting TSC-1 in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

SCYA26, UNQ216/PRO242, CCL26, C-C motif chemokine 26, CC chemokine IMAC, Eotaxin-3, Macrophage inflammatory protein 4-alpha, Small-inducible cytokine A26, Thymic stroma chemokine-1, MIP-4-alpha, TSC-1

2 Images
Sandwich ELISA - Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (AB244925)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (AB244925)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab223594).

Sandwich ELISA - Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (AB244925)
  • sELISA

Lab

Sandwich ELISA - Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (AB244925)

Sandwich ELISA of ab244014 with the capture antibody ab244661 dilution at 2 μg/mL and detector antibody ab244925 dilution at 0.5 μg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19260-105

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Monkey

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-TSC-1 antibody [EPR19260-105] - BSA and Azide free (Detector) (ab244925) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human, Monkey samples.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tuberous Sclerosis Complex 1 (TSC-1) also known as hamartin is a protein with a molecular mass of approximately 130 kDa. It plays a direct role in cellular signaling and regulatory functions. TSC-1 is expressed broadly across tissues which suggests its importance in maintaining normal cellular physiology. It interfaces with various signaling molecules and directly interacts with TSC-2 to form a protein complex.
Biological function summary

TSC-1 plays a regulatory role in cell growth and proliferation. It forms a complex with TSC-2 also known as tuberin which is important for its function. This complex acts as a GAP (GTPase-activating protein) toward Rheb a small GTPase inhibiting the mTOR signaling pathway. The proper regulation of this pathway ensures controlled cell size and growth emphasizing the importance of the TSC-1/TSC-2 complex in cellular regulation.

Pathways

TSC-1 operates within the mTOR signaling pathway to influence cellular processes like autophagy and protein synthesis. It also relates to the PI3K/AKT pathway one that is involved in the regulation of cell survival and metabolism. By interacting with TSC-2 in these pathways TSC-1 modulates the inhibition of mTORC1 thereby impacting cellular homeostasis and growth.

Defects in TSC-1 can lead to tuberous sclerosis complex a condition characterized by benign tumors in multiple organs. Mutations in TSC-1 and its partner TSC-2 disrupt normal cellular functions leading to this disease. Additionally aberrations in the mTOR pathway influenced by TSC-1 malfunction have associations with certain cancers highlighting the importance of TSC-1 in maintaining cell proliferation and signaling balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemoattractant for eosinophils and basophils (PubMed : 10415065, PubMed : 10488147). Acts as a ligand for C-C chemokine receptor CCR3 which triggers Ca(2+) mobilization in eosinophils (PubMed : 10415065, PubMed : 10488147, PubMed : 11425309). Also acts as a ligand for CX3C chemokine receptor CX3CR1, inducing cell chemotaxis (PubMed : 20974991).
See full target information CCL26
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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