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AB254711

Anti-TSEN2 antibody

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(1 Publication)

Rabbit Polyclonal TSEN2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TSEN2 aa 1-100.

View Alternative Names

SEN2, TSEN2, tRNA-splicing endonuclease subunit Sen2, tRNA-intron endonuclease Sen2, HsSen2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-TSEN2 antibody (AB254711)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TSEN2 antibody (AB254711)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling TSEN2 using ab254711 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSEN2 antibody (AB254711)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSEN2 antibody (AB254711)

Paraffin-embedded human cerebellum tissue stained for TSEN2 using ab254711 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-TSEN2 antibody (AB254711)
  • WB

Supplier Data

Western blot - Anti-TSEN2 antibody (AB254711)

Lane 1:

Western blot - Anti-TSEN2 antibody (ab254711)

Lanes 2 - 3:

Western blot - Anti-TSEN2 antibody (ab254711) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

RT4 (Human urinary bladder cancer cell line) whole cell lysate

Lane 3:

U-251MG sp whole cell lysate

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TSEN2 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q8NCE0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSEN2 or tRNA splicing endonuclease subunit 2 is a protein that plays a role in the processing of tRNA molecules. It acts as a component of the tRNA splicing endonuclease complex aiding in the precise cutting of precursor tRNA to remove introns. TSEN2's molecular mass is approximately 54 kDa. This protein is expressed in various tissues in the human body with significant expression reported in tissues that perform high levels of protein synthesis like the brain and testes.
Biological function summary

The TSEN2 protein is involved in the maturation of transfer RNA molecules by forming part of the tRNA splicing endonuclease complex. This complex consists of four subunits: TSEN2 TSEN15 TSEN34 and TSEN54. The precise function of TSEN2 within this complex is to help identify and cut specific sites within the tRNA introns so the molecules can be correctly processed ensuring functional tRNAs are available for translation processes. The activities of TSEN2 are critical for maintaining the accuracy of protein synthesis in cells.

Pathways

The TSEN2 protein participates in the tRNA processing pathway which has implications for overall protein synthesis. This pathway is important for the maturation of tRNA and subsequent translation. Another pathway that TSEN2 is involved with is the RNA splicing pathway. Proteins related to this pathway include TSEN34 and TSEN54 which also play roles in removing introns.

Genetic alterations in TSEN2 are linked to conditions like pontocerebellar hypoplasia a neurological disorder characterized by brain development issues. Mutations that affect TSEN2 and its partner proteins in the endonuclease complex like TSEN54 can lead to improper tRNA processing contributing to the pathogenesis of this disorder. Additionally TSEN2 mutations have associations with disorders where neurological function and development are disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constitutes one of the two catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3'-cyclic phosphate and 5'-OH termini. There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. Isoform 1 probably carries the active site for 5'-splice site cleavage. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events. Isoform 2 is responsible for processing a yet unknown RNA substrate. The complex containing isoform 2 is not able to cleave pre-tRNAs properly, although it retains endonucleolytic activity.
See full target information TSEN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bioengineered 13:5103-5112 PubMed35164665

2022

RAB27B inhibits proliferation and promotes apoptosis of leukemic cells via 3-Hydroxy butyrate dehydrogenase 2 (BDH2).

Applications

Unspecified application

Species

Unspecified reactive species

Can Meng,Li Huang,Xiangjun Fu,Bin Wu,Lie Lin
View all publications
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