Rabbit Polyclonal TSG101 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TSG101 aa 200-300.
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- Scientific reports 13:214472023Porcine milk exosomes modulate the immune functions of CD14 monocytes in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Ávila Morales,Daria De Leonardis,Joel Filipe,Rafaela Furioso Ferreira,Alessandro Agazzi,Helga Sauerwein,Marcello Comi,Vladimir Mrljak,Cristina Lecchi,Fabrizio CecilianiPubMed 38052991
- Journal of cellular physiology 238:1020-10352023Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinglei Wang,Yangyang Pan,Meng Wang,Ruihua Xu,Xiaohong Han,Rui Ma,Ling Zhao,Tongxiang Zhang,Yaying Wang,Tian Zhao,Tianyi Ding,Abdul Rasheed Baloch,Libin Wang,Yan Cui,Sijiu YuPubMed 37013674
- Frontiers in veterinary science 9:10831742023Plasma small extracellular vesicles from dogs affected by cutaneous mast cell tumors deliver high levels of miR-21-5p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClarissa Zamboni,Valentina Zamarian,Damiano Stefanello,Roberta Ferrari,Luigi Auletta,Samantha Milanesi,Samuele Mauri,Valeria Grieco,Fabrizio Ceciliani,Cristina LecchiPubMed 36704706
- Nutrients 13:2021GG Derived Extracellular Vesicles Modulate Gut Microbiota and Attenuate Inflammatory in DSS-Induced Colitis Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingjun Tong,Xinyi Zhang,Haining Hao,Qiqi Liu,Zihan Zhou,Xi Liang,Tongjie Liu,Pimin Gong,Lanwei Zhang,Zhengyuan Zhai,Yanling Hao,Huaxi YiPubMed 34684320
- Theranostics 11:8570-85862021Milk-derived extracellular vesicles alleviate ulcerative colitis by regulating the gut immunity and reshaping the gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLingjun Tong,Haining Hao,Zhe Zhang,Youyou Lv,Xi Liang,Qiqi Liu,Tongjie Liu,Pimin Gong,Lanwei Zhang,Fangfang Cao,Giorgia Pastorin,Chuen Neng Lee,Xiaoyuan Chen,Jiong-Wei Wang,Huaxi YiPubMed 34373759
- Vaccines 8:2020Distinct miRNA Profile of Cellular and Extracellular Vesicles Released from Chicken Tracheal Cells Following Avian Influenza Virus Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKelsey O'Dowd et. al.PubMed 32764349
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human TSG101 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q99816
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chicken | Predicted |
| Chimpanzee | Predicted |
| Cow | Predicted |
| Dog | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
| Xenopus laevis | Predicted |
| Xenopus tropicalis | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Chicken, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).
Alternative names
Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101
Recommended products
Rabbit Polyclonal TSG101 antibody. Suitable for WB and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TSG101 aa 200-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human TSG101 aa 200-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q99816
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab225877 was affinity purified using an epitope specific to TSG101 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.
- Biological function summary
TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.
- Pathways
TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.
- Associated diseases and disorders
Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-TSG101 antibody (ab225877)
All lanes: Western blot - Anti-TSG101 antibody (ab225877) at 0.04 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 44 kDa
Exposure time: 3min
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