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Rabbit Recombinant Monoclonal TSG101 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

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Images

Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (AB207567), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (AB207567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (AB207567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (AB207567), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (AB207567), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Expected
Rat
Tested
Expected
Expected
Expected

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

This product gave a positive signal in HeLa fixed with 100% methanol (5 min).

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

5 products for Alternative Version

Target data

Function

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).

Alternative names

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

Recommended products

Rabbit Recombinant Monoclonal TSG101 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7130(B)
Purification technique
Affinity purification Protein A
Dissociation constant
4 x 10-12 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab207567 is the carrier-free version of Anti-TSG101 antibody [EPR7130(B)] ab125011.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.

Biological function summary

TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.

Pathways

TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.

Associated diseases and disorders

Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:20 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Immunocytochemistry analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:50 dilution (4.5 µg/ml). Cells were fixed in 100% Methanol and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue sections labeling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling TSG101 with Purified Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1:300 dilution (0.73 µg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Western blot - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The doublet are consistent with what have been described in the literature (PMID: 27882925, 31684046, 32932791, 11427703).

    Image produced using the purified version.

    All lanes: Western blot - Anti-TSG101 antibody [EPR7130(B)] (Anti-TSG101 antibody [EPR7130(B)] ab125011) at 1/1000 dilution

    Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

    Lane 3: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 6: Mouse heart lysate at 20 µg

    Lane 7: Rat heart lysate at 20 µg

    Lane 8: Mouse brain lysate at 20 µg

    Lane 9: Rat brain lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 44 kDa

    Observed band size: 47 kDa, 52 kDa

  • Western blot - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Western blot - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation. Different batches of Anti-TSG101 antibody [EPR7130(B)] ab125011 were tested on Mouse heart lysate at 1.0 μg/ml. 15 μg of lysate was loaded in each lane. Bands observed at 45 kDa.

    This data was generarted using the unpurified format.

    All lanes: Western blot - Anti-TSG101 antibody [EPR7130(B)] (Anti-TSG101 antibody [EPR7130(B)] ab125011)

    Predicted band size: 44 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TSG101 antibody [EPR7130(B)] - BSA and Azide free (ab207567)

    Anti-TSG101 antibody [EPR7130(B)] ab125011 staining TSG101 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-TSG101 antibody [EPR7130(B)] ab125011 at 1 μg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 μg/ml. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
    This data was developed using Anti-TSG101 antibody [EPR7130(B)] ab125011, the same antibody clone in a different buffer formulation.

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