AB133586

Anti-TSG101 antibody [EPR7131(B)]

RabMAb
Recombinant
20ul selling size
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(86 Publications)

Rabbit Recombinant Monoclonal TSG101 antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 86 publications.

View Alternative Names

Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101

4 Images

Lab

Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

ab133586 at 1/40 immunoprecipitating TSG101 in K562 (Human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate observed at 44 KDa (lanes 1 and 2).

Lane 1 (input) : K562 whole cell lysate 10μg

Lane 2 (+) : ab133586 + K562 whole cell lysate.

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab133586 in whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (ab133586)

Predicted band size: 44 kDa

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Unknown

Western blot - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

Note : Dilute primary antibody in 1% BSA.

All lanes:

Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/1000 dilution

Lane 1:

PC12 cell lysate at 10 µg

Lane 2:

NIH 3T3 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 44 kDa

false

Unknown

Western blot - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

Blocking/Diluting buffer 5% NFDM/TBST

All lanes:

Western blot - Anti-TSG101 antibody [EPR7131(B)] (ab133586) at 1/2000 dilution

Lane 1:

K562 whole cell lysate at 20 µg

Lane 2:

Jurkat whole cell lysate at 20 µg

Lane 3:

PC-12 whole cell lysate at 20 µg

Lane 4:

NIH/3T3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 44 kDa

Observed band size: 44 kDa

false

OI-RD Scanning - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-TSG101 antibody [EPR7131(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7131(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.

Biological function summary

TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.

Pathways

TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.

Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed : 22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed : 22315426).

See full target information Tumor susceptibility gene 101 protein

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Publications (86)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 31:281 PubMed40849639

2025

CAF-derived exosomes drive the FGF4/SHH feedback loop by encapsulating GREM1 in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xianqiao Wu,Wei Chen,Tianzheng Fang,Ziyuan Chen,Shuai Fang,Chengwei Zhou

Scientific reports 15:29273 PubMed40784956

2025

CAFs exosomal circFOXO1 promotes TNBC autophagy and radioresistance via miR-27a-3p/BNIP3 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xueqiong Xun,Huiyong Hu,Qing Liu,Ruijun Su,Jun Ai

Journal of orthopaedic translation 53:187-205 PubMed40678612

2025

PPARγ controls ESCRT-dependent fibroblast-like synoviocyte exosome biogenesis and alleviates chondrocyte osteoarthritis mediated by exosomal ANXA1.

Applications

Unspecified application

Species

Unspecified reactive species

Shuangshuo Jia,Yue Yang,Jiabao Liu,Ziyuan Wang,Lunhao Bai

RSC advances 15:23670-23680 PubMed40630682

2025

Profiling characteristics of plasma exosomal miRNAs across cognitive stages: from normalcy to mild cognitive impairment and Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Chen,Silu Chen,Liangliang Chen,Hong Zheng,Jing Nie,Lingyan Yang,Xia Li,Kang Ju

Cell communication and signaling : CCS 23:246 PubMed40437612

2025

Identification of genetically engineered strategies to manipulate nano-platforms presenting immunotherapeutic ligands for alleviating primary ovarian insufficiency progression.

Applications

Unspecified application

Species

Unspecified reactive species

Guannan Zhou,Yuanyuan Gu,Menglei Zhang,Jingxin Ding,Guanming Lu,Keqin Hua,Fang Shen

Cancer & metabolism 13:24 PubMed40410860

2025

Improved VPS4B O-GlcNAc modification triggers lipid droplets transferring from adipocytes to nasopharyngeal carcinoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Haimeng Yin,Ying Shan,Qin Zhu,Ling Yuan,Feng Ju,Yu Shi,Yumo Han,Rui Wu,Tian Xia,Kaiwen Zhang,Yiwen You,Bo You

Stem cell research & therapy 16:253 PubMed40394699

2025

Exosomes derived from adipose-derived stem cells alleviate acute radiation-induced dermatitis through up-regulating hyaluronic acid synthase 1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Meijia Li,Yuan Tian,Xiaotian Wang,Di Sun,Haiqian Xu,Xinyue Wang,Xinyue Chen,Lijun Hao

Discover oncology 16:797 PubMed40382517

2025

Nebulized inhalation of extracellular vesicles containing SPOCK2 suppresses lung adenocarcinoma progression via MAPK inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Ningning Liu,Chuanqin Xu,Jing Wang,Liyang Dong,Shuang Yang,Junhong Jiang

BMC genomics 26:305 PubMed40155807

2025

Aromatase reduces sperm motility by down-regulating the expression of proteins related to ATP synthesis in seminal plasma extracellular vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Xuliang Luo,Yan Guo,Xuelian Li,Zi Mei,Haobo Zhou,Ping Qiu,Haoxin Wang,Yan Chen,Yanzhang Gong

Journal of nanobiotechnology 23:215 PubMed40098176

2025

Protective effects of bone marrow mesenchymal stem cell-derived exosomes loaded cerium dioxide nanoparticle against deoxynivalenol-induced liver damage.

Applications

Unspecified application

Species

Unspecified reactive species

Zitong Meng,Mingmeng Tang,Shiyin Xu,Xiaolei Zhou,Zixuan Zhang,Liunan Yang,Andreas K Nüssler,Liegang Liu,Wei Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSG101 antibody [EPR7131(B)]

Immunoprecipitation - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

Western blot - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

Western blot - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

OI-RD Scanning - Anti-TSG101 antibody [EPR7131(B)] (AB133586)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (86)

Product promise