Goat Recombinant Monoclonal TSG101 antibody. Suitable for ICC/IF, IHC-P and reacts with Mouse, Human samples.
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
ICC/IF | IHC-P | |
---|---|---|
Human | Tested | Tested |
Mouse | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
Species Human | Dilution info 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). It may also play a role in the extracellular release of microvesicles that differ from the exosomes (PubMed:22315426).
Tumor susceptibility gene 101 protein, ESCRT-I complex subunit TSG101, TSG101
Goat Recombinant Monoclonal TSG101 antibody. Suitable for ICC/IF, IHC-P and reacts with Mouse, Human samples.
IgG
Goat
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR7130(B)
Affinity purification Protein G
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This goat monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (Anti-TSG101 antibody [EPR7130(B)] ab125011). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
TSG101 also known as Tumor Susceptibility Gene 101 is a protein involved in intracellular trafficking specifically notable within the process of endosomal sorting. It is a component of the ESCRT-I (Endosomal Sorting Complex Required for Transport-I) complex. TSG101 is approximately 46 kDa and is expressed ubiquitously across various tissues. The mechanistic function of TSG101 involves the recognition and ubiquitination of cargo proteins an important step in the formation of multivesicular bodies.
TSG101 plays a critical role in the sorting and trafficking of proteins destined for lysosomal degradation or recycling back to the plasma membrane. It is an essential part of the ESCRT machinery which also includes other components like VPS37 and VPS28. TSG101 interacts with specific domains and motifs on target proteins facilitating the recognition and recruitment of these proteins into vesicles. Its involvement in membrane budding and scission makes it important for maintaining cellular homeostasis and protein quality control.
TSG101 is integrally involved in the ESCRT pathway and is associated with processes like viral budding and cytokinesis. Within these pathways it collaborates with proteins such as ALIX and the ESCRT-II component VPS25 to mediate vesicle formation and facilitate the trafficking of ubiquitinated cargo. The ESCRT pathway is vital in cellular responses such as membrane repair and is important for the life cycle of certain enveloped viruses which utilize host cell machinery for budding.
Defects or alterations in TSG101 expression have correlations with tumor progression and neurodegenerative diseases. Disruptions in the function of TSG101 can affect cellular processes potentially contributing to conditions like cancer and Alzheimer's disease. For example the aberrant activity of TSG101 may interact with proteins like Hrs and ubiquitin ligases Mdm2 in the context of oncogenic signaling pathways which can drive tumor growth and metastasis. Understanding these interactions is key to uncovering potential therapeutic targets within these diseases.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
ab320807 staining TSG101 in NIH/3T3 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab320807 at 1µg/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunofluorescence staining of TSG101 in sections of formalin-fixed paraffin-embedded human colon*.
Performed on a Leica BOND. The section was pre-treated using heat mediated antigen retrieval with sodium citrate (pH6.0) for 20 minutes. The section was then incubated at room temperature for 1 hour with ab320807 at 1/100 dilution and then incubated for 1 hour with Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed ab150133 Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.
ab320807 staining TSG101 in HeLa cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal donkey serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab320807 at 1µg/ml (shown in green) and Alexa Fluor® 594 Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab202272, Alexa Fluor® 594 Rabbit monoclonal [EP1332Y] to alpha Tubulin - Microtubule Marker (shown in pseudocolour magenta). Cells were then incubated with Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com