Rabbit Recombinant Monoclonal TSG6 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Images
![Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--western-blot-img910.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383)")
![Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--immunoprecipitation-img11816.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383)")
![Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--flow-cytometry-intracellular-img27450.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383)")
![Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--western-blot-img16152.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (AB274383)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Tested | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Expected | Tested | Not recommended | Expected |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human, Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Major regulator of extracellular matrix organization during tissue remodeling (PubMed:15917224, PubMed:18042364, PubMed:26823460). Catalyzes the transfer of a heavy chain (HC) from inter-alpha-inhibitor (I-alpha-I) complex to hyaluronan. Cleaves the ester bond between the C-terminus of the HC and GalNAc residue of the chondroitin sulfate chain in I-alpha-I complex followed by transesterification of the HC to hyaluronan. In the process, potentiates the antiprotease function of I-alpha-I complex through release of free bikunin (PubMed:15917224, PubMed:16873769, PubMed:20463016). Acts as a catalyst in the formation of hyaluronan-HC oligomers and hyaluronan-rich matrix surrounding the cumulus cell-oocyte complex, a necessary step for oocyte fertilization (PubMed:26468290). Assembles hyaluronan in pericellular matrices that serve as platforms for receptor clustering and signaling. Enables binding of hyaluronan deposited on the surface of macrophages to LYVE1 on lymphatic endothelium and facilitates macrophage extravasation. Alters hyaluronan binding to functionally latent CD44 on vascular endothelium, switching CD44 into an active state that supports leukocyte rolling (PubMed:15060082, PubMed:26823460). Modulates the interaction of chemokines with extracellular matrix components and proteoglycans on endothelial cell surface, likely preventing chemokine gradient formation (PubMed:27044744). In a negative feedback mechanism, may limit excessive neutrophil recruitment at inflammatory sites by antagonizing the association of CXCL8 with glycosaminoglycans on vascular endothelium (PubMed:24501198). Has a role in osteogenesis and bone remodeling. Inhibits BMP2-dependent differentiation of mesenchymal stem cell to osteoblasts (PubMed:16771708, PubMed:18586671). Protects against bone erosion during inflammation by inhibiting TNFSF11/RANKL-dependent osteoclast activation (PubMed:18586671).
Alternative names
TSG6, TNFAIP6, Tumor necrosis factor-inducible gene 6 protein, Hyaluronate-binding protein, TNF-stimulated gene 6 protein, Tumor necrosis factor alpha-induced protein 6, TSG-6, TNF alpha-induced protein 6
Recommended products
Rabbit Recombinant Monoclonal TSG6 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17582-45
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab274383 is the carrier-free version of Anti-TSG6 antibody [EPR17582-45] ab267469.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSG6 also known as tumor necrosis factor-inducible gene 6 protein plays an important role in inflammation and tissue repair. This protein has a molecular mass of approximately 35 kDa. TSG6 expresses in various tissues especially in response to inflammation or tissue injury. Such expression is significant in cells like macrophages and fibroblasts as well as in tissues like the synovial lining and skin.
- Biological function summary
TSG6 contributes to the modulation of the inflammatory response and tissue remodeling. It does this by interacting with molecules such as hyaluronan which is an important component of the extracellular matrix. During inflammatory responses TSG6 forms a complex with inter-α-inhibitor a serine protease inhibitor enhancing its stability and function. Through these interactions TSG6 helps to regulate the extracellular environment during tissue repair processes.
- Pathways
The protein TSG6 participates actively in the inflammatory pathway and the hyaluronan metabolism pathway. It influences inflammatory events by modulating the activity of inflammatory cytokines affecting processes like leukocyte recruitment. TSG6 works closely with proteins such as TNF-α and IL-1 both integral to the inflammatory response. In the hyaluronan pathway TSG6 influences the structural organization of the matrix affecting cell migration and adhesion.
- Associated diseases and disorders
TSG6 relates to conditions like osteoarthritis and rheumatoid arthritis. In these disorders inflammation and tissue degradation play central roles. TSG6 interacts with proteins like matrix metalloproteinases which are involved in tissue breakdown and remodeling. By modulating these interactions TSG6 can potentially influence disease progression or resolution making it an interesting target for therapeutic intervention in inflammatory diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--western-blot-img910.jpg "Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)")
Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).
The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TSG6 antibody [EPR17582-45] ab267469)
All lanes: Western blot - Anti-TSG6 antibody [EPR17582-45] (Anti-TSG6 antibody [EPR17582-45] ab267469) at 1/1000 dilution
All lanes: Rat amniotic membrane tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 120 kDa, 36 kDa
![Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--immunoprecipitation-img11816.jpg "Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)")
Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
TSG6 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate with Anti-TSG6 antibody [EPR17582-45] ab267469 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TSG6 antibody [EPR17582-45] ab267469 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours) whole cell lysate 10 ug.
Lane 2: Anti-TSG6 antibody [EPR17582-45] ab267469 IP in THP-1 (treated as above).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TSG6 antibody [EPR17582-45] ab267469 in THP-1 (treated as above) whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 41 seconds
This blot was developed using a higher sensitivity ECL substrate.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TSG6 antibody [EPR17582-45] ab267469).
All lanes: Immunoprecipitation - Anti-TSG6 antibody [EPR17582-45] (Anti-TSG6 antibody [EPR17582-45] ab267469)
Predicted band size: 31 kDa
Observed band size: 36 kDa
![Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--flow-cytometry-intracellular-img27450.jpg "Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)")
Flow Cytometry (Intracellular) - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized THP-1 (Human monocytic leukemia monocyte) (treated with 100ng/ml lipopolysaccharide (LPS) for 1h, then add BFA for 4h) (Red) / Untreated control (Green) cells labelling TSG6 with Anti-TSG6 antibody [EPR17582-45] ab267469 at 1/500 dilution (0.1ug) (Red, Green) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TSG6 antibody [EPR17582-45] ab267469).
![Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383), expandable thumbnail](https://content.abcam.com/products/images/tsg6-antibody-epr17582-45-bsa-and-azide-free-ab274383--western-blot-img16152.jpg "Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)")
Western blot - Anti-TSG6 antibody [EPR17582-45] - BSA and Azide free (ab274383)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Band of 36KDa represents the monomeric TSG6. Band of 120KDa likely represents a stable covalent complex formed between TSG6 and one of the heavy chains of inter-α-inhibitor or pre-α-inhibitor (PMID: 22351758, 11420253).
The expression profile observed is consistent with what has been described in the literature (PMID: 22351758, 11420253, 19389798).
Negative control: A549 (PMID: 19389798).
Exposure time: 3 minutes
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-TSG6 antibody [EPR17582-45] ab267469).
All lanes: Western blot - Anti-TSG6 antibody [EPR17582-45] (Anti-TSG6 antibody [EPR17582-45] ab267469) at 1/1000 dilution
Lane 1: THP-1 (human monocytic leukemia monocyte), whole cell lysate at 20 µg
Lane 2: THP-1 (treated with 100ng/mg LPS for 3 hours, then treated with BFA for 2 hours), whole cell lysate at 20 µg
Lane 3: A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 31 kDa
Observed band size: 120 kDa, 36 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com