AB224386

Anti-TSGA2 antibody

Name: Anti-TSGA2 antibody (ab224386) | Abcam
Brand: Abcam Limited
SKU: AB224386

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(1 Publication)

Rabbit Polyclonal TSGA2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus.

View Alternative Names

TSA2, TSGA2, RSPH1, Radial spoke head 1 homolog, Cancer/testis antigen 79, Male meiotic metaphase chromosome-associated acidic protein, Meichroacidin, Testis-specific gene A2 protein, CT79

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (AB224386)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (AB224386)

Paraffin-embedded human testis tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (AB224386)

Paraffin-embedded human fallopian tube tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-TSGA2 antibody (AB224386)

All lanes:

Western blot - Anti-TSGA2 antibody (ab224386) at 1/100 dilution

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate

Lane 2:

TSGA2 overexpression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)

Predicted band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSGA2 also known as testis-specific gene antigen 2 is a protein encoded by the TSGA2 gene. Its molecular mass is approximately 67 kDa. TSGA2 shows high expression in testicular tissues indicating a role related to reproductive biology. It is less often found in other tissues under normal conditions which suggests a specialized function in male germ cells.

Biological function summary

TSGA2 plays a role in the organization of cellular structures during spermatogenesis. This protein participates in the formation of sperm-specific elements. TSGA2 potentially interacts with components of the cytoskeleton facilitating the organized shape and function of mature sperm.

Pathways

TSGA2 integrates within the spermatogenesis process. The protein contributes to the structure and motility development of spermatozoa associating with proteins involved in microtubule dynamics such as tubulins. It functions alongside dynein and kinesin which are motor proteins critical for movement within cells ensuring sperm development happens correctly.

TSGA2 has connections to infertility specifically that which affects spermatogenesis. Abnormal expression or mutations in TSGA2 can disrupt normal sperm development leading to reduced fertility in males. Some studies suggest links with proteins like DAZL another important player in germ cell regulation appealing as a potential area for exploring diagnostic and therapeutic avenues in male infertility.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia.

See full target information RSPH1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of medical research 29:257 PubMed38689322

2024

Overexpressed KCNK1 regulates potassium channels affecting molecular mechanisms and biological pathways in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Xiao-Song Chen,Ying Wei,Xiao-Min Wang,Xian-Jin Chen,Bang-Teng Chi,Lin-Qing Huang,Rong-Quan He,Zhi-Guang Huang,Qi Li,Gang Chen,Juan He,Mei Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSGA2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (AB224386)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (AB224386)

Western blot - Anti-TSGA2 antibody (AB224386)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise