Rabbit Polyclonal TSGA2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WYR4
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/2500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Functions as part of axonemal radial spoke complexes that play an important part in the motility of sperm and cilia.
Alternative names
TSA2, TSGA2, RSPH1, Radial spoke head 1 homolog, Cancer/testis antigen 79, Male meiotic metaphase chromosome-associated acidic protein, Meichroacidin, Testis-specific gene A2 protein, CT79
Recommended products
Rabbit Polyclonal TSGA2 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human RSPH1 aa 200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8WYR4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSGA2 also known as testis-specific gene antigen 2 is a protein encoded by the TSGA2 gene. Its molecular mass is approximately 67 kDa. TSGA2 shows high expression in testicular tissues indicating a role related to reproductive biology. It is less often found in other tissues under normal conditions which suggests a specialized function in male germ cells.
- Biological function summary
TSGA2 plays a role in the organization of cellular structures during spermatogenesis. This protein participates in the formation of sperm-specific elements. TSGA2 potentially interacts with components of the cytoskeleton facilitating the organized shape and function of mature sperm.
- Pathways
TSGA2 integrates within the spermatogenesis process. The protein contributes to the structure and motility development of spermatozoa associating with proteins involved in microtubule dynamics such as tubulins. It functions alongside dynein and kinesin which are motor proteins critical for movement within cells ensuring sperm development happens correctly.
- Associated diseases and disorders
TSGA2 has connections to infertility specifically that which affects spermatogenesis. Abnormal expression or mutations in TSGA2 can disrupt normal sperm development leading to reduced fertility in males. Some studies suggest links with proteins like DAZL another important player in germ cell regulation appealing as a potential area for exploring diagnostic and therapeutic avenues in male infertility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (ab224386)
Paraffin-embedded human testis tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.
Western blot - Anti-TSGA2 antibody (ab224386)
All lanes: Western blot - Anti-TSGA2 antibody (ab224386) at 1/100 dilution
Lane 1: Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 2: TSGA2 overexpression HEK-293T lysate (Co-expressed with a C-terminal myc-DDK tag, ~3.1 kDa)
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSGA2 antibody (ab224386)
Paraffin-embedded human fallopian tube tissue stained for TSGA2 using ab224386 at 1/1000 dilution in immunohistochemical analysis.
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