Skip to main content

Rabbit Recombinant Monoclonal TSH beta antibody. Suitable for mIHC, WB, IHC-P and reacts with Human, Rabbit, Recombinant full length protein - Human samples. Cited in 2 publications.


Images

Western blot - Anti-TSH beta antibody [EPR8198] (AB155958), expandable thumbnail
  • Western blot - Anti-TSH beta antibody [EPR8198] (AB155958), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (AB155958), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] (AB155958), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIPWBIHC-P
Human
Tested
Not recommended
Expected
Tested
Rabbit
Expected
Not recommended
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rabbit
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Rabbit, Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rabbit
Dilution info
1/1000 - 1/10000
Notes

-

Species
Recombinant full length protein - Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250 - 1/500
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rabbit
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Indispensable for the control of thyroid structure and metabolism.

Additional Targets

TSH beta

Alternative names

Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, Thyrotropin beta chain, TSH-B, TSH-beta, TSHB

Recommended products

Rabbit Recombinant Monoclonal TSH beta antibody. Suitable for mIHC, WB, IHC-P and reacts with Human, Rabbit, Recombinant full length protein - Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR8198
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TSH beta also known as thyrotropin beta is a subunit of the thyroid-stimulating hormone (TSH). This hormone is a glycoprotein with a significant role in regulating the endocrine system. With a molecular mass of approximately 15 kDa TSH beta is one of the two subunits that together form the functional TSH. It is produced and expressed by the anterior pituitary gland. The beta subunit confers the hormone its specificity by interacting with the TSH receptor on thyroid cells.

Biological function summary

Thyrotropin beta ensures the thyroid gland functions properly by stimulating it to release thyroxine (T4) and triiodothyronine (T3). These thyroid hormones are essential for regulating metabolism in the body. TSH beta as a part of the TSH complex must combine with the alpha subunit to exhibit full biological activity. The production and secretion of TSH are tightly controlled by the hypothalamus-pituitary-thyroid axis which adjusts TSH levels based on the body's hormone requirements.

Pathways

TSH beta has a prominent role in the hypothalamus-pituitary-thyroid axis which is fundamental in thyroid hormone regulation. This pathway involves TRH (thyrotropin-releasing hormone) from the hypothalamus which stimulates the anterior pituitary to secrete TSH. In turn TSH interacts with its receptor on the thyroid gland promoting thyroid hormone synthesis. The pathway includes feedback mechanisms where increasing levels of T3 and T4 inhibit both TRH and TSH release maintaining homeostasis.

Associated diseases and disorders

TSH beta is linked to conditions like hyperthyroidism and hypothyroidism which result from imbalances in thyroid hormone levels. In hyperthyroidism excess production of T3 and T4 occurs often due to TSH receptor overstimulation while in hypothyroidism inadequate production might arise from insufficient TSH levels leading to decreased thyroid hormone output. Disorders with TSH beta involvement may also relate to antibodies in conditions like Graves' disease where autoantibodies target the TSH receptor causing thyroid dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TSH beta antibody [EPR8198] (ab155958), expandable thumbnail

    Western blot - Anti-TSH beta antibody [EPR8198] (ab155958)

    All lanes: Western blot - Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution

    All lanes: Rabbit pituitary tissue lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Western blot - Anti-TSH beta antibody [EPR8198] (ab155958), expandable thumbnail

    Western blot - Anti-TSH beta antibody [EPR8198] (ab155958)

    All lanes: Western blot - Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution

    All lanes: Human recombinant TSH alpha/beta protein at 0.01 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 16 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (ab155958), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (ab155958)

    Immunohistochemical analysis of paraffin-embedded Human pituitary tissue, labeling TSH beta with ab155958 at 1/250 dilution.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] (ab155958), expandable thumbnail

    Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] (ab155958)

    Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (Anti-ACTH antibody [57] - N-terminal ab233954; green; Opal™520) at 1:5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1:500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (Anti-Growth Hormone antibody [EPR11047(B)] ab155276; gray; Opal™690) at 1:5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining: in the order of Anti-Growth Hormone antibody [EPR11047(B)] ab155276, Anti-ACTH antibody [57] - N-terminal ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used. 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com