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AB155958

Anti-TSH beta antibody [EPR8198]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Advanced Validation
  • Recombinant
  • What is this?

1

(1 Review)

|

(4 Publications)

Rabbit Recombinant Monoclonal TSH beta antibody. Suitable for mIHC, WB, IHC-P and reacts with Human, Rabbit, Recombinant full length protein - Human samples. Cited in 4 publications.

View Alternative Names

Thyrotropin subunit beta, Thyroid-stimulating hormone subunit beta, Thyrotropin beta chain, TSH-B, TSH-beta, TSHB, TSHB, CHNG4, Thyrotropin beta subunit, Thyroid stimulating hormone beta subunit, Thyroid stimulating hormone, beta precursor

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (AB155958)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] (AB155958)

Immunohistochemical analysis of paraffin-embedded Human pituitary tissue labeling TSH beta with ab155958 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] (AB155958)
  • mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] (AB155958)

Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (ab233954; green; Opal™520) at 1 : 5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1 : 500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (ab155276; gray; Opal™690) at 1 : 5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab155276, ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.

Western blot - Anti-TSH beta antibody [EPR8198] (AB155958)
  • WB

Unknown

Western blot - Anti-TSH beta antibody [EPR8198] (AB155958)

All lanes:

Western blot - Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution

All lanes:

Rabbit pituitary tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

Western blot - Anti-TSH beta antibody [EPR8198] (AB155958)
  • WB

Unknown

Western blot - Anti-TSH beta antibody [EPR8198] (AB155958)

All lanes:

Western blot - Anti-TSH beta antibody [EPR8198] (ab155958) at 1/1000 dilution

All lanes:

Human recombinant TSH alpha/beta protein at 0.01 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

  • Carrier free

    Anti-TSH beta antibody [EPR8198] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8198

Isotype

IgG

Carrier free

No

Reacts with

Rabbit, Human

Applications

WB, IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "1/500", "mIHC-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p>" }, "Rabbit": { "mIHC-species-checked": "guaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "mIHC-species-checked": "notRecommended", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSH beta also known as thyrotropin beta is a subunit of the thyroid-stimulating hormone (TSH). This hormone is a glycoprotein with a significant role in regulating the endocrine system. With a molecular mass of approximately 15 kDa TSH beta is one of the two subunits that together form the functional TSH. It is produced and expressed by the anterior pituitary gland. The beta subunit confers the hormone its specificity by interacting with the TSH receptor on thyroid cells.
Biological function summary

Thyrotropin beta ensures the thyroid gland functions properly by stimulating it to release thyroxine (T4) and triiodothyronine (T3). These thyroid hormones are essential for regulating metabolism in the body. TSH beta as a part of the TSH complex must combine with the alpha subunit to exhibit full biological activity. The production and secretion of TSH are tightly controlled by the hypothalamus-pituitary-thyroid axis which adjusts TSH levels based on the body's hormone requirements.

Pathways

TSH beta has a prominent role in the hypothalamus-pituitary-thyroid axis which is fundamental in thyroid hormone regulation. This pathway involves TRH (thyrotropin-releasing hormone) from the hypothalamus which stimulates the anterior pituitary to secrete TSH. In turn TSH interacts with its receptor on the thyroid gland promoting thyroid hormone synthesis. The pathway includes feedback mechanisms where increasing levels of T3 and T4 inhibit both TRH and TSH release maintaining homeostasis.

TSH beta is linked to conditions like hyperthyroidism and hypothyroidism which result from imbalances in thyroid hormone levels. In hyperthyroidism excess production of T3 and T4 occurs often due to TSH receptor overstimulation while in hypothyroidism inadequate production might arise from insufficient TSH levels leading to decreased thyroid hormone output. Disorders with TSH beta involvement may also relate to antibodies in conditions like Graves' disease where autoantibodies target the TSH receptor causing thyroid dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Indispensable for the control of thyroid structure and metabolism.
See full target information Thyrotropin subunit beta
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 4:100934 PubMed36754052

2023

Single-cell sequencing identifies differentiation-related markers for molecular classification and recurrence prediction of PitNET.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Zhang,Boyuan Yao,Xin Long,Zhengyuan Chen,Min He,Yue Wu,Nidan Qiao,Zengyi Ma,Zhao Ye,Yichao Zhang,Shun Yao,Ye Wang,Haixia Cheng,Hong Chen,Hongying Ye,Yongfei Wang,Yimin Li,Jianhua Chen,Zhaoyun Zhang,Fan Guo,Yao Zhao

The Journal of pathology 258:49-57 PubMed35657600

2022

Low-rank fusion convolutional neural network for prediction of remission after stereotactic radiosurgery in patients with acromegaly: a proof-of-concept study.

Applications

Unspecified application

Species

Unspecified reactive species

Nidan Qiao,Damin Yu,Guoqing Wu,Qilin Zhang,Boyuan Yao,Min He,Hongying Ye,Zhaoyun Zhang,Yongfei Wang,Hanfeng Wu,Yao Zhao,Jinhua Yu

Nature communications 11:5275 PubMed33077725

2020

Single-cell transcriptomics identifies divergent developmental lineage trajectories during human pituitary development.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Zhang,Yueli Cui,Xinyi Ma,Jun Yong,Liying Yan,Ming Yang,Jie Ren,Fuchou Tang,Lu Wen,Jie Qiao

Cellular logistics 4:e986399 PubMed25767741

2015

Interaction with the effector dynamin-related protein 1 (Drp1) is an ancient function of Rab32 subfamily proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina G Ortiz-Sandoval,Sarah C Hughes,Joel B Dacks,Thomas Simmen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com