Anti-TSH beta antibody [EPR8198] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH beta antibody [EPR8198] - BSA and Azide free (AB249213)

This data was developed using ab155958, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human pituitary tissue, labeling TSH beta with ab155958 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-TSH beta antibody [EPR8198] - BSA and Azide free (AB249213)

Fluorescence multiplex immunohistochemical analysis of human pituitary gland tissue (formalin-fixed paraffin-embedded section). Panel A shows merged staining of anti-ACTH stained on corticotrophs (ab233954; green; Opal™520) at 1 : 5000 (0.214 μg/ml) [Panel B], anti-TSH beta stained on thyrotrophs (ab155958; red; Opal™570) at 1 : 500 (1.728 μg/ml) [Panel C], and anti-Growth Hormone stained on somatotrophs (ab155276; gray; Opal™690) at 1 : 5000 (0.044 μg/ml) [Panel D] on human pituitary gland. DAPI was used as a nuclear counter stain. Followed by Opal Polymer HRP Ms + Rb secondary antibody. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. The section was incubated in three rounds of staining : in the order of ab155276, ab233954, and ab155958 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins was used.  This data was developed using ab155958, the same antibody clone in a different buffer formulation.

• WB

Unknown

Western blot - Anti-TSH beta antibody [EPR8198] - BSA and Azide free (AB249213)

This data was developed using ab155958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSH beta antibody [EPR8198] (<a href='/en-us/products/primary-antibodies/tsh-beta-antibody-epr8198-ab155958'>ab155958</a>) at 1/1000 dilution

All lanes:

Rabbit pituitary tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false

• WB

Unknown

Western blot - Anti-TSH beta antibody [EPR8198] - BSA and Azide free (AB249213)

This data was developed using ab155958, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSH beta antibody [EPR8198] (<a href='/en-us/products/primary-antibodies/tsh-beta-antibody-epr8198-ab155958'>ab155958</a>) at 1/1000 dilution

All lanes:

Human recombinant TSH alpha/beta protein at 0.01 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 16 kDa

false