AB27974

Anti-TSH Receptor/TSH-R antibody [4C1]

Name: Anti-TSH Receptor/TSH-R antibody [4C1] (ab27974) | Abcam
Brand: Abcam Limited
SKU: AB27974

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(7 Publications )

Mouse Monoclonal TSH Receptor/TSH-R antibody. Suitable for IHC-P and reacts with Human samples. Cited in 7 publications.

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [4C1] (AB27974)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [4C1] (AB27974)

ab27974 staining TSH Receptor/TSH-R in Human thyroid tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C1

Isotype

IgG2a

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Ab27974 reacts with an epitope located in the extracellular region of TSH receptor.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is not required.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Ab27974 was purified by affinity chromatography, from tissue culture supernatant.

Storage buffer

pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TSH Receptor (TSH-R) also known as thyrotropin receptor is an important protein in the regulation of the thyroid gland. Its molecular mass is approximately 84 kDa. TSH-R is predominantly expressed on the surface of thyroid follicular cells. This receptor functions mechanically by binding thyroid-stimulating hormone (TSH) initiating a cascade that increases the production and release of thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). This receptor is critical in maintaining proper thyroid function.

Biological function summary

TSH-R plays an important role in the regulation of metabolic processes. As part of the endocrine system the TSH-R interacts with TSH secreted by the anterior pituitary modulating signaling pathways that contribute to cell growth differentiation and hormone production in the thyroid gland. TSH-R is a component of the G protein-coupled receptor family sharing structural similarities with other receptors in this group. Additionally its activation influences the synthesis of both T4 and T3 which are essential for normal development metabolism and thermoregulation.

Pathways

The TSH-R is important in the cyclic AMP (cAMP) signaling pathway. Upon TSH binding the receptor activates adenylate cyclase via the Gs protein resulting in cAMP production. This activation influences several downstream processes that promote thyroid hormone synthesis and secretion. Furthermore TSH-R engages in cross-talk with other pathways related to cell proliferation and survival. The receptor is linked with proteins like G proteins that mediate its signaling effects integrating into the broader network of hormonal regulation.

TSH-R is strongly associated with autoimmune thyroid diseases particularly Graves' disease and Hashimoto's thyroiditis. In Graves’ disease stimulating antibodies known as thyroid-stimulating immunoglobulins (TSI) mimic TSH and activate the TSH-R causing overproduction of thyroid hormones and leading to hyperthyroidism. In Hashimoto's thyroiditis TSH-R participates indirectly as autoantibodies can lead to receptor destruction and subsequent hypothyroidism. These conditions often involve interactions with additional immune-related proteins such as T cells and cytokines affecting overall immune tolerance in the thyroid gland.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed : 11847099, PubMed : 12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2 : GPHB5) or thyrostimulin (PubMed : 12045258). TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase (PubMed : 11847099, PubMed : 35940205, PubMed : 35940204). This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS) (PubMed : 27638195). Additionally, plays a central role in controlling thyroid cell metabolism (By similarity).

See full target information TSHR

Alternative Names

LGR3, TSHR, Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

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Publications (7)

Recent publications for all applications. Explore the full listand refine your search

Frontiers in endocrinology 15:1420024 PubMed39280007

2024

Impact of ibrutinib on inflammation in a mouse model of Graves' orbitopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Charm Kim,Jin Hwan Park,Yeon Jeong Choi,Hyung Oh Jun,Jin Kwon Chung,Tae Kwann Park,Jin Sook Yoon,Jae Wook Yang,Sun Young Jang

International journal of molecular sciences 22: PubMed34071318

2021

The Amino Acid Transporter Mct10/Tat1 Is Important to Maintain the TSH Receptor at Its Canonical Basolateral Localization and Assures Regular Turnover of Thyroid Follicle Cells in Male Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Vaishnavi Venugopalan,Alaa Al-Hashimi,Jonas Weber,Maren Rehders,Maria Qatato,Eva K Wirth,Ulrich Schweizer,Heike Heuer,François Verrey,Klaudia Brix

Oncology letters 14:7506-7512 PubMed29344196

2017

Downregulated expression of TSHR is associated with distant metastasis in thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Tianrun Liu,Qianqian Men,Xuan Su,Weichao Chen,Lan Zou,Qiuli Li,Ming Song,Dian Ouyang,Yanfeng Chen,Zhaoqu Li,Xiaoyan Fu,Ankui Yang

Molecular and clinical oncology 7:315-321 PubMed28781809

2017

A novel protein expression signature differentiates benign lipomas from well-differentiated liposarcomas.

Applications

Unspecified application

Species

Unspecified reactive species

Quang Mather,Jonathon Priego,Kristi Ward,Verma Kundan,Dat Tran,Alok Dwivedi,Brad A Bryan

Oncology letters 12:585-590 PubMed27347184

2016

Isolated double adrenocorticotropic hormone-secreting pituitary adenomas: A case report and review of the literature.

Applications

IHC-P

Species

Human

Jiujun Pu,Zhiming Wang,Hui Zhou,Ailing Zhong,Kai Jin,Lunliang Ruan,Gang Yang

Proteomics 12:3580-9 PubMed23071080

2012

Proteomic analysis of the maternal protein restriction rat model for schizophrenia: identification of translational changes in hormonal signaling pathways and glutamate neurotransmission.

Applications

Unspecified application

Species

Unspecified reactive species

Paul C Guest,Sebastian Urday,Dan Ma,Viktoria Stelzhammer,Laura W Harris,Bob Amess,Sandra Pietsch,Christin Oheim,Susan E Ozanne,Sabine Bahn

The American journal of pathology 161:195-206 PubMed12107104

2002

Expression of IP-10/CXCL10 and MIG/CXCL9 in the thyroid and increased levels of IP-10/CXCL10 in the serum of patients with recent-onset Graves' disease.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Romagnani,Mario Rotondi,Elena Lazzeri,Laura Lasagni,Michela Francalanci,Andrea Buonamano,Stefano Milani,Paolo Vitti,Luca Chiovato,Massimo Tonacchera,Antonio Bellastella,Mario Serio

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TSH Receptor/TSH-R antibody [4C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [4C1] (AB27974)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (7)

Product promise