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AB218108

Anti-TSH Receptor/TSH-R antibody [EPR19751]

1

(1 Review)

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(5 Publications)

Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108) is a rabbit monoclonal antibody detecting TSH Receptor/TSH-R in IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

LGR3, TSHR, Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)

Immunohistochemical analysis of paraffin-embedded human follicular thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on follicular thyroid adenocarcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Negative staining on human normal colon [PMID : 22289392].

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)

Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on human normal thyroid is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Negative staining on gastric cancer.

Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)

Immunohistochemical analysis of paraffin-embedded human papillary thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Membranous staining on papillary thyroid carcinoma is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Carrier free

    Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR19751

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thyroid Peroxidase/TPO antibody [EPR5380] (AB109383)

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Secondary Antibodies

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  • 5
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR19751] also available for your convenience: ab218108, Carrier free - ab251559

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TSH Receptor (TSH-R) also known as thyrotropin receptor is an important protein in the regulation of the thyroid gland. Its molecular mass is approximately 84 kDa. TSH-R is predominantly expressed on the surface of thyroid follicular cells. This receptor functions mechanically by binding thyroid-stimulating hormone (TSH) initiating a cascade that increases the production and release of thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). This receptor is critical in maintaining proper thyroid function.
Biological function summary

TSH-R plays an important role in the regulation of metabolic processes. As part of the endocrine system the TSH-R interacts with TSH secreted by the anterior pituitary modulating signaling pathways that contribute to cell growth differentiation and hormone production in the thyroid gland. TSH-R is a component of the G protein-coupled receptor family sharing structural similarities with other receptors in this group. Additionally its activation influences the synthesis of both T4 and T3 which are essential for normal development metabolism and thermoregulation.

Pathways

The TSH-R is important in the cyclic AMP (cAMP) signaling pathway. Upon TSH binding the receptor activates adenylate cyclase via the Gs protein resulting in cAMP production. This activation influences several downstream processes that promote thyroid hormone synthesis and secretion. Furthermore TSH-R engages in cross-talk with other pathways related to cell proliferation and survival. The receptor is linked with proteins like G proteins that mediate its signaling effects integrating into the broader network of hormonal regulation.

TSH-R is strongly associated with autoimmune thyroid diseases particularly Graves' disease and Hashimoto's thyroiditis. In Graves' disease stimulating antibodies known as thyroid-stimulating immunoglobulins (TSI) mimic TSH and activate the TSH-R causing overproduction of thyroid hormones and leading to hyperthyroidism. In Hashimoto's thyroiditis TSH-R participates indirectly as autoantibodies can lead to receptor destruction and subsequent hypothyroidism. These conditions often involve interactions with additional immune-related proteins such as T cells and cytokines affecting overall immune tolerance in the thyroid gland.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed : 11847099, PubMed : 12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2 : GPHB5) or thyrostimulin (PubMed : 12045258). TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase (PubMed : 11847099, PubMed : 35940205, PubMed : 35940204). This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS) (PubMed : 27638195). Additionally, plays a central role in controlling thyroid cell metabolism (By similarity).
See full target information TSHR
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genes & diseases 12:101565 PubMed40837414

2025

Menin facilitates the cell proliferation of bladder cancer via modulating the TFAP2C/β-catenin axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Shi,Xiang Pan,Shiheng Zhang,Mengyuan Wu,Meiqi Xu,Yun-Qi Li,Li Zhong,Zi-Qi Wang,Wanhai Xu,Yakun Luo

Surgery 175:199-206 PubMed37919223

2023

Thyroid-stimulating hormone receptor (TSHR) as a target for imaging differentiated thyroid cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Grayson R Gimblet,Jason Whitt,Hailey A Houson,Diana Lin,Rachael Guenter,Tejeshwar C Rao,Dezhi Wang,John Ness,Manuel Lora Gonzalez,Madisen S Murphy,Andrea Gillis,Herbert Chen,John A Copland,Saad S Kenderian,Ricardo V Lloyd,Mariusz W Szkudlinski,Suzanne E Lapi,Renata Jaskula-Sztul

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 36:100332 PubMed37716507

2023

Somatostatin Receptor Type 2 and Thyroid-Stimulating Hormone Receptor Expression in Oncocytic Thyroid Neoplasms: Implications for Prognosis and Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Gillis,Rui Zheng-Pywell,Chandler McLeod,Dezhi Wang,John M Ness,Rachael Guenter,Jason Whitt,Tomas A Prolla,Herbert Chen,Manuel Lora Gonzalez,Bart Rose,Ricardo V Lloyd,Renata Jaskula-Sztul,Diana Lin

European thyroid journal 12: PubMed37352166

2023

Iodine avidity in papillary and poorly differentiated thyroid cancer is predicted by immunohistochemical and molecular work-up.

Applications

Unspecified application

Species

Unspecified reactive species

Joachim N Nilsson,Jonathan Siikanen,Vincenzo Condello,Kenbugul Jatta,Ravi Saini,Christel Hedman,Catharina Ihre Lundgren,C Christofer Juhlin

Endocrine journal 67:1007-1017 PubMed32522910

2020

False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization.

Applications

Unspecified application

Species

Unspecified reactive species

Ken Takeshima,Hiroyuki Ariyasu,Shinsuke Uraki,Shuhei Morita,Yasushi Furukawa,Hidefumi Inaba,Hiroshi Iwakura,Asako Doi,Kenji Warigaya,Shin-Ichi Murata,Keisuke Enomoto,Muneki Hotomi,Takashi Akamizu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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