Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108) is a rabbit monoclonal antibody that is used to detect TSH Receptor/TSH-R in IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98435.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41047.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10458.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111176.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (AB218108)")
Publications
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- European thyroid journal 12:2023Iodine avidity in papillary and poorly differentiated thyroid cancer is predicted by immunohistochemical and molecular work-up.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoachim N Nilsson,Jonathan Siikanen,Vincenzo Condello,Kenbugul Jatta,Ravi Saini,Christel Hedman,Catharina Ihre Lundgren,C Christofer JuhlinPubMed 37352166
- Endocrine journal 67:1007-10172020False-positive staining of thyroglobulin distinguished from mixed medullary and follicular thyroid carcinoma by duplex in situ hybridization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKen Takeshima et. al.PubMed 32522910
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
5 products for Alternative Product
Target data
Function
Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:11847099, PubMed:12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Plays a central role in controlling thyroid cell metabolism (By similarity).
Alternative names
LGR3, TSHR, Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
Recommended products
Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108) is a rabbit monoclonal antibody that is used to detect TSH Receptor/TSH-R in IHC-P. Suitable for Human samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR19751
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P) in Human samples.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR19751] also available for your convenience:
ab218108, Carrier free - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free ab251559
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TSH Receptor (TSH-R) also known as thyrotropin receptor is an important protein in the regulation of the thyroid gland. Its molecular mass is approximately 84 kDa. TSH-R is predominantly expressed on the surface of thyroid follicular cells. This receptor functions mechanically by binding thyroid-stimulating hormone (TSH) initiating a cascade that increases the production and release of thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). This receptor is critical in maintaining proper thyroid function.
- Biological function summary
TSH-R plays an important role in the regulation of metabolic processes. As part of the endocrine system the TSH-R interacts with TSH secreted by the anterior pituitary modulating signaling pathways that contribute to cell growth differentiation and hormone production in the thyroid gland. TSH-R is a component of the G protein-coupled receptor family sharing structural similarities with other receptors in this group. Additionally its activation influences the synthesis of both T4 and T3 which are essential for normal development metabolism and thermoregulation.
- Pathways
The TSH-R is important in the cyclic AMP (cAMP) signaling pathway. Upon TSH binding the receptor activates adenylate cyclase via the Gs protein resulting in cAMP production. This activation influences several downstream processes that promote thyroid hormone synthesis and secretion. Furthermore TSH-R engages in cross-talk with other pathways related to cell proliferation and survival. The receptor is linked with proteins like G proteins that mediate its signaling effects integrating into the broader network of hormonal regulation.
- Associated diseases and disorders
TSH-R is strongly associated with autoimmune thyroid diseases particularly Graves' disease and Hashimoto's thyroiditis. In Graves’ disease stimulating antibodies known as thyroid-stimulating immunoglobulins (TSI) mimic TSH and activate the TSH-R causing overproduction of thyroid hormones and leading to hyperthyroidism. In Hashimoto's thyroiditis TSH-R participates indirectly as autoantibodies can lead to receptor destruction and subsequent hypothyroidism. These conditions often involve interactions with additional immune-related proteins such as T cells and cytokines affecting overall immune tolerance in the thyroid gland.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98435.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)
Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on human normal thyroid is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41047.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)
Immunohistochemical analysis of paraffin-embedded human follicular thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on follicular thyroid adenocarcinoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10458.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)
Immunohistochemical analysis of paraffin-embedded human papillary thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Membranous staining on papillary thyroid carcinoma is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative staining on human normal colon [PMID: 22289392].
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-ab218108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111176.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] (ab218108)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling TSH Receptor/TSH-R with ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Negative staining on gastric cancer.
Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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