Rabbit Recombinant Monoclonal TSH Receptor/TSH-R antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98435.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41047.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10458.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82740.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111176.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (AB251559)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target data
Function
Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin (PubMed:11847099, PubMed:12045258). Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin (PubMed:12045258). The activity of this receptor is mediated by G proteins which activate adenylate cyclase (PubMed:11847099). Plays a central role in controlling thyroid cell metabolism (By similarity).
Alternative names
LGR3, TSHR, Thyrotropin receptor, Thyroid-stimulating hormone receptor, TSH-R
Recommended products
Rabbit Recombinant Monoclonal TSH Receptor/TSH-R antibody. Carrier free. Suitable for IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19751
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251559 is the carrier-free version of Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The TSH Receptor (TSH-R) also known as thyrotropin receptor is an important protein in the regulation of the thyroid gland. Its molecular mass is approximately 84 kDa. TSH-R is predominantly expressed on the surface of thyroid follicular cells. This receptor functions mechanically by binding thyroid-stimulating hormone (TSH) initiating a cascade that increases the production and release of thyroid hormones such as thyroxine (T4) and triiodothyronine (T3). This receptor is critical in maintaining proper thyroid function.
- Biological function summary
TSH-R plays an important role in the regulation of metabolic processes. As part of the endocrine system the TSH-R interacts with TSH secreted by the anterior pituitary modulating signaling pathways that contribute to cell growth differentiation and hormone production in the thyroid gland. TSH-R is a component of the G protein-coupled receptor family sharing structural similarities with other receptors in this group. Additionally its activation influences the synthesis of both T4 and T3 which are essential for normal development metabolism and thermoregulation.
- Pathways
The TSH-R is important in the cyclic AMP (cAMP) signaling pathway. Upon TSH binding the receptor activates adenylate cyclase via the Gs protein resulting in cAMP production. This activation influences several downstream processes that promote thyroid hormone synthesis and secretion. Furthermore TSH-R engages in cross-talk with other pathways related to cell proliferation and survival. The receptor is linked with proteins like G proteins that mediate its signaling effects integrating into the broader network of hormonal regulation.
- Associated diseases and disorders
TSH-R is strongly associated with autoimmune thyroid diseases particularly Graves' disease and Hashimoto's thyroiditis. In Graves’ disease stimulating antibodies known as thyroid-stimulating immunoglobulins (TSI) mimic TSH and activate the TSH-R causing overproduction of thyroid hormones and leading to hyperthyroidism. In Hashimoto's thyroiditis TSH-R participates indirectly as autoantibodies can lead to receptor destruction and subsequent hypothyroidism. These conditions often involve interactions with additional immune-related proteins such as T cells and cytokines affecting overall immune tolerance in the thyroid gland.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img98435.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)
This data was developed using Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human thyroid tissue labeling TSH Receptor/TSH-R with Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on human normal thyroid is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41047.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)
This data was developed using Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human follicular thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on follicular thyroid adenocarcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img10458.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)
This data was developed using Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human papillary thyroid adenocarcinoma tissue labeling TSH Receptor/TSH-R with Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Membranous staining on papillary thyroid carcinoma is observed. Counter stained with Hematoxylin. Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img82740.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)
This data was developed using Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TSH Receptor/TSH-R with Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative staining on human normal colon [PMID: 22289392]. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559), expandable thumbnail](https://content.abcam.com/products/images/tsh-receptor-tsh-r-antibody-epr19751-bsa-and-azide-free-ab251559--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111176.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSH Receptor/TSH-R antibody [EPR19751] - BSA and Azide free (ab251559)
This data was developed using Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling TSH Receptor/TSH-R with Anti-TSH Receptor/TSH-R antibody [EPR19751] ab218108 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Negative staining on gastric cancer. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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