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AB115700

Anti-TSLP antibody

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(9 Publications)

Rabbit Polyclonal TSLP antibody. Suitable for IHC-P, ELISA, WB and reacts with Mouse samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Mouse Tslp aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Thymic stromal lymphopoietin, Thymic stroma-derived lymphopoietin, Tslp

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)

ab115700, at 5 µg/ml, staining TSLP in Formalin-fixed, Paraffin-embedded Mouse Liver tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)

ab115700, at 5 µg/ml, staining TSLP in Formalin-fixed, Paraffin-embedded Mouse Spleen tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Western blot - Anti-TSLP antibody (AB115700)
  • WB

Unknown

Western blot - Anti-TSLP antibody (AB115700)

Lane 1:

Western blot - Anti-TSLP antibody (ab115700) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TSLP antibody (ab115700) at 1 µg/mL

Lane 3:

Western blot - Anti-TSLP antibody (ab115700) at 2 µg/mL

All lanes:

A20 cell lysate

Predicted band size: 18 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB, ELISA

applications

Immunogen

Synthetic Peptide within Mouse Tslp aa 50 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9JIE6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells (By similarity).
See full target information Tslp
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Animals : an open access journal from MDPI 13: PubMed37444013

2023

Canine Mesenchymal-Stem-Cell-Derived Extracellular Vesicles Attenuate Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Byong Seung Cho,Sung-Bae Kim,Sokho Kim,Beomseok Rhee,Jungho Yoon,Jae Won Lee

Annals of translational medicine 10:209 PubMed35280398

2022

Polyinosinic:polycytidylic acid aggravates calcipotriol-induced atopic dermatitis-like skin lesions in mice by increasing the expression of thymic stromal lymphopoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Haoyue Wan,Huixue Yang,Mingjing Wei,Wenqi Chen

Frontiers in pharmacology 12:722730 PubMed34616298

2021

Modulation of the Gut Microbiota by - Shapes the Immune Responses of Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Jaemoo Chun,So Min Lee,You Mee Ahn,Min-Gyung Baek,Hana Yi,Sarah Shin,Jeeyoun Jung

Allergy, asthma & immunology research 11:560-571 PubMed31172724

2019

Protease-Activated Receptors 2-Antagonist Suppresses Asthma by Inhibiting Reactive Oxygen Species-Thymic Stromal Lymphopoietin Inflammation and Epithelial Tight Junction Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Jung Kim,Seung Hwa Lee,Sekyoo Jeong,Soo Jong Hong

Scientific reports 8:11129 PubMed30042397

2018

The role of metallothionein in a dinitrofluorobenzene-induced atopic dermatitis-like murine model.

Applications

Unspecified application

Species

Unspecified reactive species

Jin-Zhu Guo,Wen-Hui Wang,Lin-Feng Li,Shao-Min Yang,Jing Wang

The Journal of allergy and clinical immunology 142:793-803.e8 PubMed29132961

2017

Experimental asthma persists in IL-33 receptor knockout mice because of the emergence of thymic stromal lymphopoietin-driven IL-9 and IL-13 type 2 innate lymphoid cell subpopulations.

Applications

Unspecified application

Species

Unspecified reactive species

Mukesh Verma,Sucai Liu,Lidia Michalec,Anand Sripada,Magdalena M Gorska,Rafeul Alam

Medicine 96:e7516 PubMed28746197

2017

Role of thymic stromal lymphopoietin in the pathogenesis of lumbar disc degeneration.

Applications

ICC/IF

Species

Human

Yu Wang,Xiao-Dong Yi,Chun-De Li

Journal of molecular and cellular cardiology 76:33-45 PubMed25117469

2014

TSLPR deficiency attenuates atherosclerotic lesion development associated with the inhibition of TH17 cells and the promotion of regulator T cells in ApoE-deficient mice.

Applications

Unspecified application

Species

Mouse

Chun Wu,Shaolin He,Yudong Peng,Kishan Kumar Kushwaha,Jing Lin,Jiangchuan Dong,Boyuan Wang,Jibin Lin,Shengshuai Shan,Jing Liu,Kai Huang,Dazhu Li

The Journal of allergy and clinical immunology 134:836-847.e11 PubMed24996260

2014

MicroRNA-146a alleviates chronic skin inflammation in atopic dermatitis through suppression of innate immune responses in keratinocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Rebane,Toomas Runnel,Alar Aab,Julia Maslovskaja,Beate Rückert,Maya Zimmermann,Mario Plaas,Jaanika Kärner,Angela Treis,Maire Pihlap,Uku Haljasorg,Helen Hermann,Nikoletta Nagy,Lajos Kemeny,Triin Erm,Külli Kingo,Mei Li,Mark P Boldin,Cezmi A Akdis
View all publications
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