AB188766

Anti-TSLP antibody

Name: Anti-TSLP antibody (ab188766) | Abcam
Brand: Abcam Limited
SKU: AB188766
Price: 505 USD
Availability: InStock
Rating: 3 (1 reviews)

(1 Review)

(36 Publications)

Anti-TSLP antibody (ab188766) is a rabbit polyclonal antibody detecting TSLP in Western Blot, IHC-P. Suitable for Human, Mouse.

- Over 20 publications

View Alternative Names

Thymic stromal lymphopoietin, TSLP

5 Images

Supplier Data

Western blot - Anti-TSLP antibody (AB188766)

Lane 1:

Western blot - Anti-TSLP antibody (ab188766) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TSLP antibody (ab188766) at 1 µg/mL

Lane 3:

Western blot - Anti-TSLP antibody (ab188766) at 2 µg/mL

All lanes:

A20 cell lysate

Predicted band size: 18 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB188766)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB188766)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate tissue labeling TSLP with ab188766 at 5μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB188766)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain, cortex tissue labeling TSLP with ab188766 at 5μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB188766)

Immunofluorescent analysis of human brain tissue labeling TSLP with ab188766 at 20μg/ml.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB188766)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling TSLP with ab188766 at 5μg/ml, followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TSLP. The exact immunogen used to generate this antibody is proprietary information.

Q969D9

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>" } } }

style

left-column

Product details

What is this antibody validated in?
Anti-TSLP antibody (ab188766) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human, Mouse samples.

What is the molecular weight of TSLP?
Anti-TSLP (ab188766) specifically detects a band for TSLP (UniProt: Q969D9) at a molecular weight of 18kDa.

Trusted by the scientific community
Anti-TSLP (ab188766) was first used in a scientific publication in 2014 and has been cited over 20 times in peer-reviewed journals.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).

Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.

See full target information TSLP

style

left-column

Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 35:e2410025 PubMed40081901

2025

Ameliorative Effects of Escin on Inflammation via Glucocorticoid Receptor (GR) in Atopic Dermatitis (AD) Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

A Yeon Park,Jung Ok Lee,You Na Jang,Su-Young Kim,Ji Hye Heo,Yu-Jin Kim,Jung Min Lee,Dae Won Yoon,Beom Joon Kim,Joon Seok

Frontiers in immunology 15:1450887 PubMed39867900

2025

NLRP3 deficiency aggravated DNFB-induced chronic itch by enhancing type 2 immunity IL-4/TSLP-TRPA1 axis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Ting Huang,Zuo-Ming Chen,Zhe Peng,Yu Wang,Fan Yang,Yang Tang,Zi Li,Li Wan

Chinese medicine 19:177 PubMed39722037

2024

Indole-3-carbinol alleviates allergic skin inflammation via periostin/thymic stromal lymphopoietin suppression in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun-Mi Kang,Hye-Min Kim,Junho Lee,Jong-Suep Baek,Minho Lee,Hyo-Jin An

Heliyon 10:e37935 PubMed39391467

2024

Anti-inflammatory Capacity of a Medicinal herb extract, , on and models-induced atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Hye-Min Kim,Yun-Mi Kang,Bo-Ram Jin,Minho Lee,Hyo-Jin An

International archives of allergy and immunology 186:203-211 PubMed39299223

2024

Baicalin Attenuates Type 2 Immune Responses in a Mouse Allergic Asthma Model through Inhibiting the Production of Thymic Stromal Lymphopoietin.

Applications

Unspecified application

Species

Unspecified reactive species

Zhisen Zeng,Yaoxin Ruan,Haoran Ying,Jie Wang,Huangbin Wang,Shuzhen Chen

eLife 13: PubMed39213029

2024

Defining cell type-specific immune responses in a mouse model of allergic contact dermatitis by single-cell transcriptomics.

Applications

Unspecified application

Species

Unspecified reactive species

Youxi Liu,Meimei Yin,Xiaoting Mao,Shuai Wu,Shuangping Wei,Shujun Heng,Yichun Yang,Jinwen Huang,Zhuolin Guo,Chuan Li,Chao Ji,Liu Hu,Wenjie Liu,Ling-Juan Zhang

Journal of microbiology and biotechnology 34:765-773 PubMed38247218

2024

Ozonated Sunflower Oil (OSO) Alleviates Inflammatory Responses in Oxazolone-Induced Atopic Dermatitis (AD)-Like Mice and LPS-Treated RAW 264.7 Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Su-Young Kim,Jung Ok Lee,Sue Lee,Jihye Heo,Kyung-Hyun Cho,Ashutosh Bahuguna,Kwang-Ho Yoo,Beom Joon Kim

British journal of cancer 130:585-596 PubMed38172534

2024

TSLP enhances progestin response in endometrial cancer via androgen receptor signal pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mu Lv,Yuan Xu,Peiqin Chen,Jingjie Li,Zuoshu Qin,Baozhu Huang,Yong Liu,Xiang Tao,Jun Xiang,Yanqiu Wang,Youji Feng,Wenxin Zheng,Zhenbo Zhang,Linxia Li,Hong Liao

Journal of the European Academy of Dermatology and Venereology : JEADV 38:895-903 PubMed38084871

2023

Mechanism underlying pruritus in recessive dystrophic epidermolysis bullosa: Role of interleukin-31 from mast cells and macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Sang Gyun Lee,Song-Ee Kim,In-Hye Jeong,Sang Eun Lee

Frontiers in immunology 14:1273182 PubMed38053996

2023

Epidermal keratinocyte-specific STAT3 deficiency aggravated atopic dermatitis-like skin inflammation in mice through TSLP upregulation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao-Yuan Wang,Yu-Xin Zheng,Fan Xu,Ying-Zhe Cui,Xue-Yan Chen,Si-Qi Chen,Bing-Xi Yan,Yuan Zhou,Min Zheng,Xiao-Yong Man

View all publications

style

left-column

style

left-column

style

right-column

Alternative Products

Primary Antibodies

AB47943

Anti-TSLP antibody

Western blot - Anti-TSLP antibody (AB47943)

Primary Antibodies

AB84247

Biotin Anti-TSLP antibody

Sandwich ELISA - Biotin Anti-TSLP antibody (AB84247)

Primary Antibodies

AB184638

Anti-TSLP antibody

Western blot - Anti-TSLP antibody (AB184638)

Primary Antibodies

AB229251

Anti-TSLP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB229251)

Primary Antibodies

AB115700

Anti-TSLP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)

Complementary Products

Assay Kits

AB192149

Human TSLP ELISA Kit

SimpleStep

ELISA - Human TSLP ELISA Kit (AB192149)

Primary Antibodies

AB187060

Anti-IL-33 antibody [EPR17831]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (AB187060)

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

Primary Antibodies

AB955

Anti-CD68 antibody [KP1]

Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD68 antibody [KP1] (AB955)

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com