JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229251

Anti-TSLP antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal TSLP antibody. Suitable for WB, IHC-P, ELISA and reacts with Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TSLP.

View Alternative Names

Thymic stromal lymphopoietin, TSLP

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB229251)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB229251)

Paraffin-embedded human colon carcinoma tissue stained for TSLP using ab229251 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-TSLP antibody (AB229251)
  • WB

Supplier Data

Western blot - Anti-TSLP antibody (AB229251)

15% SDS-PAGE

All lanes:

Western blot - Anti-TSLP antibody (ab229251) at 1/500 dilution

All lanes:

Human kidney tissue extract at 30 µg

Predicted band size: 18 kDa

true

ELISA - Anti-TSLP antibody (AB229251)
  • ELISA

Supplier Data

ELISA - Anti-TSLP antibody (AB229251)

An ELISA plate is coated with 50 µL of TSLP recombinant protein at concentration of 2 µg/mL. The coated protein is detected with anti-TSLP antibody at concentration ranged from 31.25 to 500 ng/mL.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TSLP. The exact immunogen used to generate this antibody is proprietary information.

Q969D9

style
left-column

Alternative Products

Primary Antibodies

AB47943

Anti-TSLP antibody

Western blot - Anti-TSLP antibody (AB47943)

  • 3
  • 1
Primary Antibodies

AB84247

Biotin Anti-TSLP antibody

Sandwich ELISA - Biotin Anti-TSLP antibody (AB84247)

  • 0
  • 0
Primary Antibodies

AB184638

Anti-TSLP antibody

Western blot - Anti-TSLP antibody (AB184638)

  • 0
  • 0
Primary Antibodies

AB115700

Anti-TSLP antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSLP antibody (AB115700)

  • 0
  • 0
Primary Antibodies

AB188766

Anti-TSLP antibody

Western blot - Anti-TSLP antibody (AB188766)

  • 3
  • 1

Complementary Products

Assay Kits

AB192149

Human TSLP ELISA Kit

SimpleStep

ELISA - Human TSLP ELISA Kit (AB192149)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB221155

Anti-Filaggrin antibody [EPR21892]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Filaggrin antibody [EPR21892] (AB221155)

  • 5
  • 3
Primary Antibodies

AB187060

Anti-IL-33 antibody [EPR17831]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR17831] (AB187060)

  • 5
  • 2
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.
See full target information TSLP
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 19:e3001352 PubMed34491982

2021

The antiviral state has shaped the CpG composition of the vertebrate interferome to avoid self-targeting.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew E Shaw,Suzannah J Rihn,Nardus Mollentze,Arthur Wickenhagen,Douglas G Stewart,Richard J Orton,Srikeerthana Kuchi,Siddharth Bakshi,Mila Rodriguez Collados,Matthew L Turnbull,Joseph Busby,Quan Gu,Katherine Smollett,Connor G G Bamford,Elena Sugrue,Paul C D Johnson,Ana Filipe Da Silva,Alfredo Castello,Daniel G Streicker,David L Robertson,Massimo Palmarini,Sam J Wilson
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com