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AB47943

Anti-TSLP antibody

3

(1 Review)

|

(34 Publications)

Anti-TSLP antibody (ab47943) is a rabbit polyclonal antibody detecting TSLP in Western Blot, IHC-P, ICC/IF, ELISA. Suitable for Human.

- Over 30 publications
- Trusted since 2007

View Alternative Names

Thymic stromal lymphopoietin, TSLP

2 Images
Western blot - Anti-TSLP antibody (AB47943)
  • WB

Unknown

Western blot - Anti-TSLP antibody (AB47943)

The 28 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to TSLP.

All lanes:

Western blot - Anti-TSLP antibody (ab47943) at 1 µg/mL

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 18 kDa

Observed band size: 28 kDa,48 kDa,51 kDa,53 kDa

true

Exposure time: 4min

Sandwich ELISA - Anti-TSLP antibody (AB47943)
  • sELISA

Unknown

Sandwich ELISA - Anti-TSLP antibody (AB47943)

ab47943 detecting TSLP in sandwich ELISA

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, ELISA, sELISA, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TSLP.

Q969D9

Reactivity data

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Product details

What is this antibody validated in?
Anti-TSLP antibody (ab47943) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ELISA in Human samples.

What is the molecular weight of TSLP?
Anti-TSLP (ab47943) specifically detects a band for TSLP (UniProt: Q969D9) at a molecular weight of 18kDa.

Trusted by the scientific community
Anti-TSLP (ab47943) was first used in a scientific publication in 2007 and has been cited over 30 times in peer-reviewed journals.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 100ul of sterile water to 1mg/ml.
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was purified by affinity chromatography employing immobilized hTSLP matrix.
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSLP or thymic stromal lymphopoietin is a cytokine protein weighing around 27 kDa. It is also known as a hematopoietic cytokine and functions in the signaling processes of the immune system. TSLP is expressed in epithelial cells most notably in the skin lungs and gut. This cytokine holds significance due to its role in immune responses acting through its receptor complex made of TSLP receptor (TSLPR) and the interleukin-7 receptor alpha chain (IL-7Rα).
Biological function summary

TSLP plays a role in mediating immune system responses. It induces the differentiation of T-helper 2 (Th2) cells promoting a specific type of immune response. TSLP does not appear to be part of a larger protein complex but it often functions in association with the TSLPR and IL-7Rα receptor complex. It influences the activity and maturation of dendritic cells which are important for initiating immune responses.

Pathways

A mechanical understanding of TSLP reveals its involvement in both the JAK-STAT and NF-kB signaling pathways. It interacts with proteins like JAK2 and STAT5 which are important for the downstream signaling cascade leading to inflammation and immune responses. These pathways play important roles in the immune system's ability to detect and respond to environmental triggers.

TSLP has relevance in conditions like asthma and atopic dermatitis. It promotes inflammation that contributes to these allergic diseases. The dysregulation of TSLP expression can exacerbate these conditions. In asthma TSLP interacts with proteins such as interleukin-33 (IL-33) to enhance inflammatory responses in airway tissues. In atopic dermatitis connections with interleukin-4 (IL-4) are noted as both cytokines contribute to the inflammatory processes involved in the disease pathology. These associations make TSLP a potential target for therapeutic interventions in such disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Isoform 1. Cytokine that induces the release of T-cell-attracting chemokines from monocytes and, in particular, enhances the maturation of CD11c(+) dendritic cells. Can induce allergic inflammation by directly activating mast cells.. Isoform 2. May act as an antimicrobial peptide in the oral cavity and on the skin.
See full target information TSLP

Publications (34)

Recent publications for all applications. Explore the full list and refine your search

International journal of nanomedicine 19:8779-8796 PubMed39220192

2024

Molybdenum Nanoparticles Alleviate MC903-Induced Atopic Dermatitis-Like Symptoms in Mice by Modulating the ROS-Mediated NF-κB and Nrf2 /HO-1 Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Xiao,Jing Guo,Yongzhou Lu,Jin Gao,Chuanlong Jia,Minghuan Huang,Weifang Chu,Wei Yao,Peng Ning,Qiannan Xu,Nan Xu

International journal of molecular sciences 23: PubMed36430783

2022

Isosorbide Fatty Acid Diesters Have Synergistic Anti-Inflammatory Effects in Cytokine-Induced Tissue Culture Models of Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

William R Swindell,Krzysztof Bojanowski,Ratan K Chaudhuri

International journal of molecular sciences 23: PubMed36362328

2022

An Amide Alkaloid Isolated from Ameliorates OVA-Induced Allergic Asthma by Inhibiting Mast Cell Activation and Dendritic Cell Maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Jufang Jia,Mengnan Zeng,Denghui Zhu,Xinmian Jiao,Beibei Zhang,Ruolan Yang,Weisheng Feng,Xiaoke Zheng

Clinical, cosmetic and investigational dermatology 15:2313-2321 PubMed36325102

2022

A Comparison for Type 2 Cytokines and Lesional Inflammatory Infiltrations in Bullous Pemphigoid and Atopic Dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Qing Hu,Jian-Zhong Zhang

iScience 25:105150 PubMed36193049

2022

Wnt-activating human skin organoid model of atopic dermatitis induced by and its protective effects by .

Applications

Unspecified application

Species

Unspecified reactive species

Song-Yi Jung,Hyun Ju You,Min-Ji Kim,GwangPyo Ko,Seunghee Lee,Kyung-Sun Kang

Immune network 22:e35 PubMed36081523

2022

Tobacco Smoking Could Accentuate Epithelial-Mesenchymal Transition and Th2-Type Response in Patients With Chronic Rhinosinusitis With Nasal Polyps.

Applications

Unspecified application

Species

Unspecified reactive species

Ki-Il Lee,Younghwan Han,Jae-Sung Ryu,Seung Min In,Jong-Yeup Kim,Joong Su Park,Jong-Seok Kim,Juhye Kim,Jubin Youn,Seok-Rae Park

Clinical and translational gastroenterology 13:e00510 PubMed35905420

2022

Mucosal Overexpression of Thymic Stromal Lymphopoietin and Proinflammatory Cytokines in Patients With Autoimmune Atrophic Gastritis.

Applications

Unspecified application

Species

Unspecified reactive species

Marco Vincenzo Lenti,Federica Facciotti,Emanuela Miceli,Alessandro Vanoli,Giulia Fornasa,Edith Lahner,Ilaria Spadoni,Paolo Giuffrida,Giovanni Arpa,Alessandra Pasini,Laura Rovedatti,Flavio Caprioli,Cristina Travelli,Georgia Lattanzi,Laura Conti,Catherine Klersy,Maurizio Vecchi,Marco Paulli,Bruno Annibale,Gino Roberto Corazza,Maria Rescigno,Antonio Di Sabatino

The Journal of pharmacology and experimental therapeutics 380:26-33 PubMed34728559

2021

ASP7266, a Novel Antibody against Human Thymic Stromal Lymphopoietin Receptor for the Treatment of Allergic Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Mako Numazaki,Masaki Abe,Kaori Hanaoka,Emiko Imamura,Masashi Maeda,Akihiko Kimura,Jun Miyanohara,Tetsu Saito,Kazunori Arai,Hiroshi Suzuki,Yuji Saita

Cancers 13: PubMed33652749

2021

Short-Form Thymic Stromal Lymphopoietin (sfTSLP) Is the Predominant Isoform Expressed by Gynaecologic Cancers and Promotes Tumour Growth.

Applications

Unspecified application

Species

Unspecified reactive species

Loucia Kit Ying Chan,Tat San Lau,Kit Ying Chung,Chit Tam,Tak Hong Cheung,So Fan Yim,Jacqueline Ho Sze Lee,Ricky Wai Tak Leung,Jing Qin,Yvonne Yan Yan Or,Kwok Wai Lo,Joseph Kwong

Pharmacogenomics and personalized medicine 13:633-644 PubMed33244255

2020

The Relationship Between Human Papillomavirus, OFD1 and Primary Ciliogenesis in the Progression of Oropharyngeal Cancer: A Retrospective Cohort Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Xue Meng,Xin-Xin Yang,Rui-Qi Liu,Jun-Jie Bao,Yun-Jing Hou,Ji Sun,Su-Sheng Miao,Guo-Fan Qu
View all publications

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