AB181993

Anti-TSP50 antibody [EPR8675(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TSP50 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CT20, TSP50, PRSS50, Probable threonine protease PRSS50, Cancer/testis antigen 20, Serine protease 50, Testis-specific protease-like protein 50

3 Images

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling TSP50with ab181993at a 1/150 dilution, or with a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution

All lanes:

MDA-MB-435 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,43 kDa

false

Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (ab181993) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

false

Carrier free

Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8675(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSP50 also known as testis-specific protease 50 is a serine protease with a molecular mass of approximately 53 kDa. This protein is expressed mainly in human testes and has also been observed in certain cancerous tissues. TSP50 shows protease activity which may contribute to its role in cellular processes. While the precise mechanical functions remain not entirely understood TSP50 is involved in the degradation of substrates within cells suggesting a part in protein turnover or processing.

Biological function summary

The action of TSP50 is significant in the regulation of cell growth and differentiation. While not part of a well-defined complex its activity affects cellular homeostasis. The expression pattern of TSP50 mainly restricted to reproductive tissues and some cancer cell types highlights its role in reproductive functions and links to oncogenesis. Its regulation impacts cellular environments possibly promoting proliferation and invasive behavior of cancer cells.

Pathways

TSP50 engages in signaling processes tied to cell cycle regulation and apoptosis. The protein plays a part in pathways related to ubiquitin-dependent proteolysis and might interact with proteins such as p53 a major regulatory protein of the cell cycle and apoptosis. By modulating protein degradation TSP50 can influence cell fate decisions potentially tipping the balance toward growth or death depending on the cellular context.

Increased TSP50 expression associates with certain cancers including breast and prostate cancer. Its expression correlates with malignant progression tumor growth and metastasis. TSP50's interaction with proteins like BRCA1 a known factor in breast cancer risk emphasizes its role in tumor biology. These connections suggest that TSP50 could serve as a potential biomarker for cancer diagnosis or a target for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

May be involved in proteolysis through its threonine endopeptidase activity.

See full target information PRSS50

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 15:9641-9646 PubMed29928339

2018

Cardamonin reduces chemotherapy resistance of colon cancer cells via the TSP50/NF-κB pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Sen Lu,Caizhao Lin,Xiaobin Cheng,Hanju Hua,Tao Xiang,Yu Huang,Xi Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSP50 antibody [EPR8675(2)]

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Western blot - Anti-TSP50 antibody [EPR8675(2)] (AB181993)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise