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AB250559

Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TSP50 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CT20, TSP50, PRSS50, Probable threonine protease PRSS50, Cancer/testis antigen 20, Serine protease 50, Testis-specific protease-like protein 50

3 Images
Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)

This data was developed using ab181993, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed MCF7 cells labeling TSP50 with ab181993 at a 1/150 dilution, or with a rabbit monoclonal IgG isotype control. Secondary antibody used was goat anti rabbit IgG (FITC) at a 1/150 dilution.

Western blot - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)
  • WB

Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)

This data was developed using ab181993, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (<a href='/en-us/products/primary-antibodies/tsp50-antibody-epr86752-ab181993'>ab181993</a>) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 43 kDa

false

Western blot - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)
  • WB

Supplier Data

Western blot - Anti-TSP50 antibody [EPR8675(2)] - BSA and Azide free (AB250559)

This data was developed using ab181993, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSP50 antibody [EPR8675(2)] (<a href='/en-us/products/primary-antibodies/tsp50-antibody-epr86752-ab181993'>ab181993</a>) at 1/1000 dilution

All lanes:

MDA-MB-435 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 38 kDa,43 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8675(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab250559 is the carrier-free version of ab181993.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSP50 also known as testis-specific protease 50 is a serine protease with a molecular mass of approximately 53 kDa. This protein is expressed mainly in human testes and has also been observed in certain cancerous tissues. TSP50 shows protease activity which may contribute to its role in cellular processes. While the precise mechanical functions remain not entirely understood TSP50 is involved in the degradation of substrates within cells suggesting a part in protein turnover or processing.
Biological function summary

The action of TSP50 is significant in the regulation of cell growth and differentiation. While not part of a well-defined complex its activity affects cellular homeostasis. The expression pattern of TSP50 mainly restricted to reproductive tissues and some cancer cell types highlights its role in reproductive functions and links to oncogenesis. Its regulation impacts cellular environments possibly promoting proliferation and invasive behavior of cancer cells.

Pathways

TSP50 engages in signaling processes tied to cell cycle regulation and apoptosis. The protein plays a part in pathways related to ubiquitin-dependent proteolysis and might interact with proteins such as p53 a major regulatory protein of the cell cycle and apoptosis. By modulating protein degradation TSP50 can influence cell fate decisions potentially tipping the balance toward growth or death depending on the cellular context.

Increased TSP50 expression associates with certain cancers including breast and prostate cancer. Its expression correlates with malignant progression tumor growth and metastasis. TSP50's interaction with proteins like BRCA1 a known factor in breast cancer risk emphasizes its role in tumor biology. These connections suggest that TSP50 could serve as a potential biomarker for cancer diagnosis or a target for therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May be involved in proteolysis through its threonine endopeptidase activity.
See full target information PRSS50
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:458 PubMed37736555

2023

S100A6 is a potential diagnostic and prognostic biomarker for human glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Hong,Hui Zhang,Yufei Xiao,Lingwei Shen,Yun Qian
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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