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AB70007

Anti-Tspan-8 antibody

4

(1 Review)

|

(5 Publications)

Rabbit Polyclonal Tspan-8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TSPAN8 aa 50-150.

View Alternative Names

TM4SF3, TSPAN8, Tetraspanin-8, Tspan-8, Transmembrane 4 superfamily member 3, Tumor-associated antigen CO-029

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-8 antibody (AB70007)

IHC image of ab70007 staining in human colon cancer formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70007, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Immunocytochemistry/ Immunofluorescence - Anti-Tspan-8 antibody (AB70007)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Tspan-8 antibody (AB70007)

Immunofluorescence of HeLa cells treated with ab70007 at 1/500 dilution. Right panel was treated with immunising peptide.

Western blot - Anti-Tspan-8 antibody (AB70007)
  • WB

Unknown

Western blot - Anti-Tspan-8 antibody (AB70007)

All lanes:

Western blot - Anti-Tspan-8 antibody (ab70007) at 1/500 dilution

Lane 1:

293 cell extract at 30 µg

Lane 2:

COLO cell extract at 30 µg

Lane 3:

COLO cell extract at 30 µg with immunising peptide

Predicted band size: 26 kDa

Observed band size: 26 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TSPAN8 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

P19075

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tspan-8 also known as Tetraspanin-8 is a protein with a mass of about 27 kDa. This protein belongs to the tetraspanin family characterized by four transmembrane domains. Tspan-8 is expressed in various tissues including the gastrointestinal tract kidney and lung with notable expression in human tumors. It localizes to membranes where it participates in organizing specific microdomains essential for cellular activities like adhesion and migration.
Biological function summary

Tspan-8 impacts several cellular functions such as motility and invasion. It often acts as part of a complex interacting with integrins and other tetraspanins. Through these interactions Tspan-8 facilitates communication between the extracellular matrix and the cytoskeleton. This interaction affects processes like cellular attachment and extracellular matrix remodeling contributing to its functions in normal physiological and pathological conditions.

Pathways

Numerous studies tie Tspan-8 to significant biological pathways particularly those involved in signal transduction and cellular movement. It connects closely with the integrin signaling pathway influencing cellular responses to external signals. In this context Tspan-8 interacts with proteins such as integrin αvβ6 contributing to diverse cellular outcomes. Additionally its role within the epithelial-to-mesenchymal transition (EMT) pathway is relevant for its function in cell plasticity and migration.

Tspan-8 links to cancer and inflammatory conditions. Its overexpression associates with tumor progression and metastasis particularly in cancers like colorectal and pancreatic cancer. The protein's interactions with integrin partners notably integrin αvβ6 could enhance cancer cell invasiveness. Moreover inflammation-related diseases might involve Tspan-8 through its influence on inflammatory cell trafficking and invasion contributing to disease pathology. Understanding these interactions can provide insights into novel therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling (PubMed : 27180357, PubMed : 36078095). Participates thereby in diverse biological functions such as cell signal transduction, migration and protein trafficking (PubMed : 25761241). Promotes ADAM17-mediated TNF-alpha processing through recruitment of ADAM17 to tetraspanin-enriched micro-domains (TEMs) (PubMed : 36078095). Forms a complex with RICTOR and integrin alpha3/ITGA3 to mediate mTORC2 activation and AKT1 phosphorylation leading to cell migration (PubMed : 25761241). Reduces apoptosis and autophagy induced by high glucose levels through forming a complex with mTOR and RICTOR (PubMed : 35904232). Contributes to the maintenance of intestinal epithelial barrier and plays a role in the regulation of intestine inflammation by switching interferon gamma receptor 1/IFNGR1 from clathrin-dependent to lipid raft-dependent endocytosis route to limit STAT1 activation magnitude and duration (PubMed : 37204469). Acts as a modulator of the endothelin axis by associating with endothelin converting enzyme ECE1 and regulating its activity of conversion of the endothelin-1 precursor to endothelin (PubMed : 37835445).
See full target information TSPAN8
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell research 32:359-374 PubMed35197608

2022

EGFR signaling promotes nuclear translocation of plasma membrane protein TSPAN8 to enhance tumor progression via STAT3-mediated transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Lu,Liwei An,Guangjian Fan,Lijuan Zang,Weiyi Huang,Junjian Li,Jun Liu,Weiyu Ge,Yuwei Huang,Jingxuan Xu,Shaoqian Du,Yuan Cao,Tianhao Zhou,Huijing Yin,Li Yu,Shi Jiao,Hongxia Wang

Oncogene 40:4884-4893 PubMed34163029

2021

SOX9 is a critical regulator of TSPAN8-mediated metastasis in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Junjian Li,Xiaoliang Chen,Liqun Zhu,Zhenghong Lao,Tianhao Zhou,Lijuan Zang,Weiyu Ge,Mengyi Jiang,Jingxuan Xu,Yuan Cao,Shaoqian Du,Yue Yu,Guangjian Fan,Hongxia Wang

Nature communications 10:2863 PubMed31253779

2019

TSPAN8 promotes cancer cell stemness via activation of sonic Hedgehog signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Rongxuan Zhu,Olivier Gires,Liqun Zhu,Jun Liu,Junjian Li,Hao Yang,Gaoda Ju,Jing Huang,Weiyu Ge,Yi Chen,Zhimin Lu,Hongxia Wang

International journal of oncology 52:473-484 PubMed29345284

2018

Quantitative proteomics analysis of the role of tetraspanin-8 in the drug resistance of gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lan Li,Daping Yang,Dejun Cui,Yu Li,Zhao Nie,Jinglin Wang,Li Liang

Oncogene 35:4540-8 PubMed26804173

2016

Therapeutic targeting of tetraspanin8 in epithelial ovarian cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

C S Park,T-K Kim,H G Kim,Y-J Kim,M H Jeoung,W R Lee,N K Go,K Heo,S Lee
View all publications
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Product promise

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