AB215432

Anti-Tspan-9 antibody

Name: Anti-Tspan-9 antibody (ab215432) | Abcam
Brand: Abcam Limited
SKU: AB215432

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(1 Publication)

Rabbit Polyclonal Tspan-9 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TSPAN9 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

NET5, TSPAN9, Tetraspanin-9, Tspan-9, Tetraspan NET-5

3 Images

Flow Cytometry - Anti-Tspan-9 antibody (AB215432)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Tspan-9 antibody (AB215432)

Flow cytometric analysis of Human U937 cells labeling Tspan-9 with ab215432 at 1/100 dilution for 40 minutes, followed by incubation with Goat Anti-Rabbit IgG PE conjugated secondary antibody for 40 minutes (green), compared to control cells (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-9 antibody (AB215432)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-9 antibody (AB215432)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling Tspan-9 with ab215432 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-9 antibody (AB215432)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse spleen tissue labeling Tspan-9 with ab215432 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Synthetic Peptide within Human TSPAN9 aa 150 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O75954

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20 - 1/100", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Tspan-9 also known as Tetraspanin-9 is a protein involved in various cellular processes like cell adhesion proliferation and migration. This protein belongs to the tetraspanin superfamily characterized by four transmembrane domains. Tspan-9 has a molecular mass of approximately 26 kDa. Research shows that Tspan-9 is expressed in several tissues including the immune system's components such as leukocytes indicating its diverse role in immune functionality and cellular communication.

Biological function summary

Tspan-9 influences cell signaling pathways through interactions with other tetraspanins and membrane proteins. Tspan-9 associations with integrins facilitate the organization of signaling complexes that mediate cellular responses. It often forms complexes with proteins involved in maintaining the cell membrane's structural integrity. These interactions show its role in controlling cellular behavior relevant to growth and communication between cells.

Pathways

Tspan-9 plays a role in the regulation of integrin-mediated signaling and the PI3K/AKT signaling pathway. Tspan-9 interacts with proteins such as CD9 and integrins affecting pathways instrumental in cell survival and proliferation. These interactions contribute to cellular adhesion dynamics demonstrating Tspan-9's role in facilitating important cellular processes.

Alterations in Tspan-9 expression have a connection to certain cancer types. Specifically Tspan-9 modulates processes in tumor development and metastasis. Its interactions with proteins like CD9 have implications in cancer pathology affecting tumor growth and invasive potential. Additionally abnormalities in Tspan-9 function or regulation may link to immunological disorders reflecting its role in immune system modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information TSPAN9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 11:170-179 PubMed29858052

2018

Cryptotanshinone Protects Cartilage against Developing Osteoarthritis through the miR-106a-5p/GLIS3 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Quanbo Ji,Dengbin Qi,Xiaojie Xu,Yameng Xu,Stuart B Goodman,Lei Kang,Qi Song,Zhongyi Fan,William J Maloney,Yan Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Tspan-9 antibody

Flow Cytometry - Anti-Tspan-9 antibody (AB215432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-9 antibody (AB215432)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Tspan-9 antibody (AB215432)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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