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AB276063

Anti-TSPAN1 antibody [010] - BSA and Azide free

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Rabbit Recombinant Monoclonal TSPAN1 antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Tetraspanin-1 aa 100 to C-terminus.

View Alternative Names

Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)

Immunochemical staining of human TSPAN1 in human prostate with ab276063 at 1/10000 dilution, formalin-fixed paraffin embedded sections).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)

Immunochemical staining of human TSPAN1 in human rectal cancer with ab276063 at 1/10000, formalin-fixed paraffin embedded sections.

Flow Cytometry (Intracellular) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TSPAN1 antibody [010] - BSA and Azide free (AB276063)

Intracellular flow cytometric analysis of Human TSPAN1 expression on SW480 cells. The cells were treated according to manufacturers manual, stained with purifiedab276063 then a FITC-conjugated secondary antibody. The Fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

010

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human Tetraspanin-1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60635

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/20000", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/25 - 1/100", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSPAN1 also known as Tetraspanin-1 is a protein encoded by the TSPAN1 gene. It is part of the tetraspanin family and is characterized by four transmembrane domains. This protein has a mass of approximately 25 kDa. TSPAN1 is mainly expressed in epithelial tissues including the skin lung and gastrointestinal tract. By forming complexes with other proteins TSPAN1 integrally contributes to various cellular processes like proliferation adhesion and migration through its role in organizing the cell membrane microdomains.
Biological function summary

TSPAN1 interacts with cell surface proteins to regulate important cellular activities. A notable characteristic of TSPAN1 is its involvement in the formation of the tetraspanin-enriched microdomains (TEMs). These microdomains facilitate interactions between various cell surface receptors and signaling molecules. This formation often includes integrins which play an important role in cell adhesion and signal transduction. TSPAN1 influences processes such as cell migration invasion and proliferation typically by modulating the activity of these integrins within the TEMs.

Pathways

TSPAN1 modulates the PI3K/Akt signaling pathway. The protein interacts with other tetraspanins and integrins to affect this signaling cascade which is critical for cell growth and survival. Additionally TSPAN1 connects with other signaling pathways that involve proteins like E-cadherin affecting cell-to-cell junctions and impacting epithelial integrity and function. These pathways highlight the diverse roles of TSPAN1 in maintaining cellular structure and function through its interactions.

TSPAN1 shows strong associations with cancer progression particularly in gastric and colorectal cancers. The protein's role in cell proliferation and migration links it to these malignancies often showing overexpression in tumor tissues. In these cancers TSPAN1 interacts with proteins like integrins and PI3K driving oncogenic processes. Alterations in TSPAN1 expression or function may relate to disease prognosis indicating its potential as a biomarker or therapeutic target in cancer-related research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed : 30066932, PubMed : 30291375). Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways (By similarity). Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK (PubMed : 30291375). Also promotes the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process (PubMed : 21836059).
See full target information Tetraspanin-1
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Product promise

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