Rabbit Polyclonal TSPAN1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Tetraspanin-1 aa 100 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Tetraspanin-1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60635
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed:30066932, PubMed:30291375). Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways (By similarity). Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK (PubMed:30291375). Promotes also the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process (PubMed:21836059).
Alternative names
Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1
Recommended products
Rabbit Polyclonal TSPAN1 antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Tetraspanin-1 aa 100 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: Tris glycine, 10% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Tetraspanin-1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O60635
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSPAN1 also known as Tetraspanin-1 is a protein encoded by the TSPAN1 gene. It is part of the tetraspanin family and is characterized by four transmembrane domains. This protein has a mass of approximately 25 kDa. TSPAN1 is mainly expressed in epithelial tissues including the skin lung and gastrointestinal tract. By forming complexes with other proteins TSPAN1 integrally contributes to various cellular processes like proliferation adhesion and migration through its role in organizing the cell membrane microdomains.
- Biological function summary
TSPAN1 interacts with cell surface proteins to regulate important cellular activities. A notable characteristic of TSPAN1 is its involvement in the formation of the tetraspanin-enriched microdomains (TEMs). These microdomains facilitate interactions between various cell surface receptors and signaling molecules. This formation often includes integrins which play an important role in cell adhesion and signal transduction. TSPAN1 influences processes such as cell migration invasion and proliferation typically by modulating the activity of these integrins within the TEMs.
- Pathways
TSPAN1 modulates the PI3K/Akt signaling pathway. The protein interacts with other tetraspanins and integrins to affect this signaling cascade which is critical for cell growth and survival. Additionally TSPAN1 connects with other signaling pathways that involve proteins like E-cadherin affecting cell-to-cell junctions and impacting epithelial integrity and function. These pathways highlight the diverse roles of TSPAN1 in maintaining cellular structure and function through its interactions.
- Associated diseases and disorders
TSPAN1 shows strong associations with cancer progression particularly in gastric and colorectal cancers. The protein's role in cell proliferation and migration links it to these malignancies often showing overexpression in tumor tissues. In these cancers TSPAN1 interacts with proteins like integrins and PI3K driving oncogenic processes. Alterations in TSPAN1 expression or function may relate to disease prognosis indicating its potential as a biomarker or therapeutic target in cancer-related research.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (ab230658)
Formalin-fixed, paraffin-embedded human uterus tissue stained for TSPAN1 with ab230658 at 5 μg/ml in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (ab230658)
Formalin-fixed, paraffin-embedded human cerebellum tissue stained for TSPAN1 with ab230658 at 5 μg/ml in immunohistochemical analysis.
Western blot - Anti-TSPAN1 antibody (ab230658)
12% SDS-PAGE gel.
All lanes: Western blot - Anti-TSPAN1 antibody (ab230658) at 1/500 dilution
All lanes: NCI-H1299 (Human lung carcinoma cell line) whole cell lysate at 30 µg
Predicted band size: 26 kDa
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