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AB254730

Anti-TSPAN1 antibody

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(3 Publications)

Rabbit Polyclonal TSPAN1 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Tetraspanin-1 aa 100 to C-terminus.

View Alternative Names

Tetraspanin-1, Tspan-1, Tetraspan NET-1, Tetraspanin TM4-C, TSPAN1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-TSPAN1 antibody (AB254730)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TSPAN1 antibody (AB254730)

PFA fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells labeling TSPAN1 using ab254730 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)

Paraffin-embedded human skeletal muscle tissue stained for TSPAN1 using ab254730 at 1/200 dilution in immunohistochemical analysis. No positivity in myocytes as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)

Paraffin-embedded human colon tissue stained for TSPAN1 using ab254730 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)

Paraffin-embedded human prostate tissue stained for TSPAN1 using ab254730 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN1 antibody (AB254730)

Paraffin-embedded human kidney tissue stained for TSPAN1 using ab254730 at 1/200 dilution in immunohistochemical analysis.

Western blot - Anti-TSPAN1 antibody (AB254730)
  • WB

Supplier Data

Western blot - Anti-TSPAN1 antibody (AB254730)

Western blot analysis lableing TSPAN1 with ab254730 at 0.04 ug/ml.

All lanes:

Western blot - Anti-TSPAN1 antibody (ab254730) at 0.04 µg/mL

Lane 1:

PC3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 26 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Tetraspanin-1 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O60635

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSPAN1 also known as Tetraspanin-1 is a protein encoded by the TSPAN1 gene. It is part of the tetraspanin family and is characterized by four transmembrane domains. This protein has a mass of approximately 25 kDa. TSPAN1 is mainly expressed in epithelial tissues including the skin lung and gastrointestinal tract. By forming complexes with other proteins TSPAN1 integrally contributes to various cellular processes like proliferation adhesion and migration through its role in organizing the cell membrane microdomains.
Biological function summary

TSPAN1 interacts with cell surface proteins to regulate important cellular activities. A notable characteristic of TSPAN1 is its involvement in the formation of the tetraspanin-enriched microdomains (TEMs). These microdomains facilitate interactions between various cell surface receptors and signaling molecules. This formation often includes integrins which play an important role in cell adhesion and signal transduction. TSPAN1 influences processes such as cell migration invasion and proliferation typically by modulating the activity of these integrins within the TEMs.

Pathways

TSPAN1 modulates the PI3K/Akt signaling pathway. The protein interacts with other tetraspanins and integrins to affect this signaling cascade which is critical for cell growth and survival. Additionally TSPAN1 connects with other signaling pathways that involve proteins like E-cadherin affecting cell-to-cell junctions and impacting epithelial integrity and function. These pathways highlight the diverse roles of TSPAN1 in maintaining cellular structure and function through its interactions.

TSPAN1 shows strong associations with cancer progression particularly in gastric and colorectal cancers. The protein's role in cell proliferation and migration links it to these malignancies often showing overexpression in tumor tissues. In these cancers TSPAN1 interacts with proteins like integrins and PI3K driving oncogenic processes. Alterations in TSPAN1 expression or function may relate to disease prognosis indicating its potential as a biomarker or therapeutic target in cancer-related research.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking (PubMed : 30066932, PubMed : 30291375). Regulates neuronal differentiation in response to NGF by facilitating NGF-mediated activation of NTRK1/TRKA receptor tyrosine kinase and subsequent downstream signaling pathways (By similarity). Plays a role in the inhibition of TNFalpha-induced apoptosis. Mechanistically, inhibits the NF-kappa-B signaling pathway by blocking phosphorylation of CHUK (PubMed : 30291375). Promotes also the stability of the thiamine transporter 1/SLC19A2 in intestinal epithelial cells leading to an increase of thiamine uptake process (PubMed : 21836059).
See full target information Tetraspanin-1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer science 114:4535-4547 PubMed37750019

2023

Tetraspanin 1 regulates papillary thyroid tumor growth and metastasis through c-Myc-mediated glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jihua Han,Changming Xie,Bo Liu,Yan Wang,Rui Pang,Wen Bi,Rinan Sheng,Guoqing He,Lingyu Kong,Jiawei Yu,Zhaoming Ding,Lili Chen,Jinliang Jia,Jiewu Zhang,Chunlei Nie

Stem cells international 2022:4041550 PubMed36187230

2022

lncRNA DARS-AS1 Modulates TSPAN1-Mediated ITGA2 Hypomethylation by Interaction with miR-194-5p Thus Promoting Ovarian Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Haoyu Gao,Beibei Chen,Li Li,Qianqing Wang,Zhihui Gao

Experimental and therapeutic medicine 22:1184 PubMed34475974

2021

Knockdown of LINC01123 inhibits cell viability, migration and invasion via miR-361-3p/TSPAN1 targeting in cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cui Li,Yujuan Li,Yuying Zhang,Hua Yan,Chengxiang Huang
View all publications
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