Rabbit Polyclonal TSPAN12 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human TSPAN12 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TSPAN12 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O95859
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Predicted |
| Mouse | Predicted |
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Regulator of cell surface receptor signal transduction. Plays a central role in retinal vascularization by regulating norrin (NDP) signal transduction. Acts in concert with norrin (NDP) to promote FZD4 multimerization and subsequent activation of FZD4, leading to promote accumulation of beta-catenin (CTNNB1) and stimulate LEF/TCF-mediated transcriptional programs. Suprisingly, it only activates the norrin (NDP)-dependent activation of FZD4, while it does not activate the Wnt-dependent activation of FZD4, suggesting the existence of a Wnt-independent signaling that also promote accumulation the beta-catenin (CTNNB1) (By similarity). Acts as a regulator of membrane proteinases such as ADAM10 and MMP14/MT1-MMP. Activates ADAM10-dependent cleavage activity of amyloid precursor protein (APP). Activates MMP14/MT1-MMP-dependent cleavage activity.
Alternative names
NET2, TM4SF12, UNQ774/PRO1568, TSPAN12, Tetraspanin-12, Tspan-12, Tetraspan NET-2, Transmembrane 4 superfamily member 12
Recommended products
Rabbit Polyclonal TSPAN12 antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human TSPAN12 aa 1-50 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TSPAN12 aa 1-50 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O95859
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSPAN12 also known as Tetraspanin-12 is a protein with a molecular weight of approximately 30 kDa. It functions mechanically as a cell surface glycoprotein involved in signal transduction. TSPAN12 belongs to the tetraspanin family characterized by four transmembrane domains. It is highly expressed in vascular endothelial cells particularly in the central nervous system and retina where it plays a role in cellular interactions and communication.
- Biological function summary
TSPAN12 influences cellular processes such as angiogenesis and neurovascular development. It acts as a part of the tetraspanin-enriched microdomain (TEM) complexes which facilitate interactions between cell surface proteins and organize them into functional signaling clusters. TSPAN12 modulates the activity of the Frizzled-4 receptor important in the Wnt/β-catenin signaling pathway thereby affecting cell proliferation and migration.
- Pathways
TSPAN12 contributes to the regulation of the Wnt signaling and VEGF signaling pathways. Within the Wnt signaling pathway TSPAN12 interacts with Frizzled-4 playing an essential role in maintaining vascular health and development. In the VEGF signaling pathway TSPAN12 influences endothelial cell behavior working alongside proteins like VEGFR-2 and Norrin essential for angiogenic processes and vascular stability.
- Associated diseases and disorders
Mutations in TSPAN12 are linked to familial exudative vitreoretinopathy (FEVR) a genetic disorder affecting retinal vascular development. TSPAN12 mutations disrupt the Wnt signaling pathway often in conjunction with mutations in proteins like Norrin and Frizzled-4 leading to an aberrant vascular structure in the retina. TSPAN12's dysfunction is also connected to retinal degenerative diseases highlighting its role as a potential target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN12 antibody (ab213678)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat brain tissue labeling TSPAN12 with ab213678 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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