AB250243

Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal TSPAN17 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

FBXO23, TM4SF17, TSPAN17, Tetraspanin-17, Tspan-17, F-box only protein 23, Tetraspan protein SB134, Transmembrane 4 superfamily member 17

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

This data was developed using ab180601, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human Transitional cell bladder carcinoma tissue labeling TSPAN17 with ab180601 at 1/100 dilution, followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Unknown

Immunoprecipitation - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

This data was developed using ab180601, the same antibody clone in a different buffer formulation.

Western blot analysis of Jurkat cell lysate immuoprecipitated with ab180601 at 1/60 dilution. Goat Anti-Rabbit IgG, (H+L) peroxidase conjugated secondary antibody used at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TSPAN17 antibody [EPR9855(2)] (<a href='/en-us/products/primary-antibodies/tspan17-antibody-epr98552-ab180601'>ab180601</a>)

Predicted band size: 30 kDa

false

Supplier Data

Western blot - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

This data was developed using ab180601, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPAN17 antibody [EPR9855(2)] (<a href='/en-us/products/primary-antibodies/tspan17-antibody-epr98552-ab180601'>ab180601</a>) at 1/20000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Lane 3:

Caco-2 cell lysate at 20 µg

Lane 4:

SW480 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 30 kDa

Observed band size: 36 kDa

false

Unconjugated

Anti-TSPAN17 antibody [EPR9855(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9855(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab250243 is the carrier-free version of ab180601.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSPAN17 also known as tetraspanin-17 is a member of the tetraspanin family of proteins. It functions mechanically as a membrane-associated protein with four transmembrane domains. The protein has a molecular weight of approximately 25 kDa. Expression of TSPAN17 occurs broadly in various tissues with a notable presence in the central nervous system and immune cells. This protein plays a role in cell signaling by forming complexes with other cell surface receptors.

Biological function summary

TSPAN17 contributes to the orchestration of cell motility and interaction processes. It is a part of the tetraspanin-enriched microdomain which facilitates the lateral organization of distinct protein complexes at the cell membrane. These complexes support processes like cell adhesion and migration suggesting TSPAN17 participation in cellular communication and signaling pathways. The involvement in these processes highlights its role in maintaining cellular dynamics and response to external stimuli.

Pathways

TSPAN17 integrates into pathways governing cell adhesion and immune response. It interacts with proteins within the integrin signaling pathway important for facilitating cell-matrix interactions and coordinating immune system activity. Through its association with integrins TSPAN17 aids in linking extracellular matrix signals to intracellular cascades like those involving phosphatidylinositol 3-kinase (PI3K) affecting cellular responses to environmental changes.

Research links TSPAN17 to neurological disorders and cancer. Alterations in its expression levels have emerged in studies of neurodegenerative diseases where abnormal activity affects cellular signaling. In cancer TSPAN17's interaction with tissue-invasive proteins such as matrix metalloproteinases (MMPs) links it to tumor progression and metastasis. Understanding TSPAN17's role provides insights into its potential as a therapeutic target in these complex diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed : 28600292, PubMed : 37516108). Seems to regulate VE-cadherin expression in endothelial cells probably through interaction with ADAM10, promoting leukocyte transmigration (PubMed : 28600292).

See full target information TSPAN17

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

Immunoprecipitation - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

Western blot - Anti-TSPAN17 antibody [EPR9855(2)] - BSA and Azide free (AB250243)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise