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AB236881

Anti-TSPAN5 antibody

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(2 Publications)

Rabbit Polyclonal TSPAN5 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TSPAN5 aa 100-250.

View Alternative Names

TM4SF9, TSPAN5, Tetraspanin-5, Tspan-5, Tetraspan NET-4, Transmembrane 4 superfamily member 9

2 Images
Western blot - Anti-TSPAN5 antibody (AB236881)
  • WB

Supplier Data

Western blot - Anti-TSPAN5 antibody (AB236881)

All lanes:

Western blot - Anti-TSPAN5 antibody (ab236881) at 1/1000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

A549 (Human lung carcinoma cell line) whole cell lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 30 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TSPAN5 antibody (AB236881)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TSPAN5 antibody (AB236881)

MCF7 (Human breast adenocarcinoma cell line) cells stained for TSPAN5 (Green) using ab236881 at a 1/100 dilution in ICC/IF. Secondary used is an Alexa-Fluor®488-conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TSPAN5 aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

P62079

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSPAN5 also known as Transmembrane 4 superfamily member 5 is a protein involved in several cellular mechanisms. It belongs to the tetraspanin family characterized by its four transmembrane domains. TSPAN5 has an approximate mass of 27 kilodaltons. The protein is broadly expressed in different tissues including the nervous system and vascular endothelial cells where it plays roles in various cellular processes.
Biological function summary

TSPAN5 interacts with other proteins to influence cell signaling adhesion and migration. It forms part of the tetraspanin web a complex network that organizes membrane proteins into functional units facilitating cell communication and structural organization. This network allows TSPAN5 to affect how cells respond to their environment and organize in multicellular structures.

Pathways

TSPAN5 plays a role in the Wnt signaling pathway which is important for cell development and growth. Its interaction with proteins like β-catenin affects how cells proliferate and differentiate. TSPAN5 is also linked to the Notch signaling pathway mediating cellular communication that influences cell fate decisions. These pathways highlight the significance of TSPAN5 in maintaining normal cellular functions and responses.

TSPAN5 shows associations with certain types of cancer and neurological disorders. In cancers like glioblastoma TSPAN5 interaction with integrins assists in tumor proliferation and metastasis. In neurological disorders it connects with proteins involved in neurotransmission such as synaptic vesicle proteins suggesting a role in conditions like Alzheimer's disease. Understanding these interactions helps target TSPAN5 in therapeutic strategies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of TspanC8 subgroup, composed of 6 members that interact with the transmembrane metalloprotease ADAM10. This interaction is required for ADAM10 exit from the endoplasmic reticulum and for enzymatic maturation and trafficking to the cell surface as well as substrate specificity. Different TspanC8/ADAM10 complexes have distinct substrates (PubMed : 26686862, PubMed : 28600292, PubMed : 37516108). Promotes ADAM10-mediated cleavage of CD44 (PubMed : 26686862). Seems to regulate VE-cadherin expression in endothelial cells probably through interaction with ADAM10, promoting leukocyte transmigration (PubMed : 28600292).
See full target information TSPAN5
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 18:5168-5184 PubMed35982894

2022

Tubule-mitophagic secretion of SerpinG1 reprograms macrophages to instruct anti-septic acute kidney injury efficacy of high-dose ascorbate mediated by NRF2 transactivation.

Applications

Unspecified application

Species

Unspecified reactive species

Yin Ni,Guo-Hua Wu,Juan-Juan Cai,Run Zhang,Yang Zheng,Jing-Quan Liu,Xiang-Hong Yang,Xue Yang,Ye Shen,Jun-Mei Lai,Xiang-Ming Ye,Shi-Jing Mo

Yonsei medical journal 63:282-291 PubMed35184431

2022

MiR-322-5p Alleviates Cell Injury and Impairment of Cognitive Function in Vascular Dementia by Targeting TSPAN5.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Jie Zhang,Bin Zhou,Huanxian Chang
View all publications
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