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Rabbit Recombinant Monoclonal TSPEAR antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (AB192023), expandable thumbnail
  • Western blot - Anti-TSPEAR antibody [EPR15505] (AB192023), expandable thumbnail
  • Western blot - Anti-TSPEAR antibody [EPR15505] (AB192023), expandable thumbnail
  • Western blot - Anti-TSPEAR antibody [EPR15505] (AB192023), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (AB192023), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Expected
Rat
Tested
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000 - 1/2000
Notes

-

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Expected
Expected

Species
Mouse
Dilution info
1/100
Notes

-

Species
Rat
Dilution info
1/100
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100 - 1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
1/100 - 1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100 - 1/200
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway (PubMed:27736875). May play a role in development or function of the auditory system (PubMed:22678063).

Alternative names

C21orf29, TSPEAR, Thrombospondin-type laminin G domain and EAR repeat-containing protein, TSP-EAR

Recommended products

Rabbit Recombinant Monoclonal TSPEAR antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Rat, Human, Mouse samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15505
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TSPEAR also known as Thrombospondin Type S Serine Protease and ARM Repeat Containing is a protein with a molecular mass of approximately 92 kDa. It functions mechanically as a protein involved in cell adhesion and communication processes. Expression of TSPEAR occurs predominantly in epithelial tissues including the skin and the gastrointestinal tract. It contains a series of ARM repeats which contribute to its functional role within the cellular structure.

Biological function summary

It participates in essential signaling pathways that regulate cell proliferation and differentiation. TSPEAR often associates with other proteins forming a complex that mediates interactions between cells and the surrounding extracellular matrix. This function is important for maintaining the stability of tissue architecture and facilitating tissue development and repair processes.

Pathways

TSPEAR plays a role in processes like the Notch signaling and Wnt signaling pathways. In the Notch signaling pathway it interacts with proteins involved in cell fate determinations assisting in the regulation of cell differentiation. Within the Wnt pathway TSPEAR associates with other proteins to influence cell growth and development. Its interaction with these pathways implies a cooperative role with proteins such as Notch receptors and beta-catenin highlighting its involvement in critical cellular communication networks.

Associated diseases and disorders

TSPEAR has been linked to conditions like Alagille syndrome and certain skin disorders. In Alagille syndrome it interacts with proteins such as JAG1 and NOTCH2 which are important for the proper signaling required in fetal development. Moreover its expression levels can alter in various dermatological conditions connecting TSPEAR to proteins involved in skin structure and repair. Understanding its role in these disorders may provide insights into therapeutic targets and interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

    All lanes: Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: 293 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

    Predicted band size: 75 kDa

    Observed band size: 80 kDa

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

    All lanes: Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/5000 dilution

    All lanes: human testis tissue lysate at 1/10 dilution

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa

    Observed band size: 80 kDa

  • Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023)

    All lanes: Western blot - Anti-TSPEAR antibody [EPR15505] (ab192023) at 1/1000 dilution

    Lane 1: C6 cell lysate at 10 µg

    Lane 2: RAW264.7 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 75 kDa

    Observed band size: 80 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] (ab192023), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] (ab192023)

    Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary at a 1/200 dilution. Counterstain: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 both at 1/500 dilution. The negative controls: Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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