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AB251105

Anti-TSPEAR antibody [EPR15505] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TSPEAR antibody. Carrier free. Suitable for ICC/IF, WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 1 publication.

View Alternative Names

C21orf29, TSPEAR, Thrombospondin-type laminin G domain and EAR repeat-containing protein, TSP-EAR

6 Images
Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/en-us/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/1000 dilution

Lane 1:

C6 cell lysate at 10 µg

Lane 2:

RAW264.7 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling TSPEAR using ab192023 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) (ab150077) was used as the secondary at a 1/200 dilution. Counterstain : ab7291 and ab150120 both at 1/500 dilution. The negative controls : Primary ab concentration (anti-TSPEAR) is 1/100 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1/500 dilution. Cells were permeabilized using 0.1% Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human stomach tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded human kidney tissue sections labeling TSPEAR using ab192023 at a 1/200 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain. Negative control uses PBS instead of primary antibody.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/en-us/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/5000 dilution

All lanes:

human testis tissue lysate at 1/10 dilution

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)
  • WB

Supplier Data

Western blot - Anti-TSPEAR antibody [EPR15505] - BSA and Azide free (AB251105)

This data was developed using ab192023, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TSPEAR antibody [EPR15505] (<a href='/en-us/products/primary-antibodies/tspear-antibody-epr15505-ab192023'>ab192023</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/100 dilution

Predicted band size: 75 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15505

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab251105 is the carrier-free version of ab192023.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSPEAR also known as Thrombospondin Type S Serine Protease and ARM Repeat Containing is a protein with a molecular mass of approximately 92 kDa. It functions mechanically as a protein involved in cell adhesion and communication processes. Expression of TSPEAR occurs predominantly in epithelial tissues including the skin and the gastrointestinal tract. It contains a series of ARM repeats which contribute to its functional role within the cellular structure.
Biological function summary

It participates in essential signaling pathways that regulate cell proliferation and differentiation. TSPEAR often associates with other proteins forming a complex that mediates interactions between cells and the surrounding extracellular matrix. This function is important for maintaining the stability of tissue architecture and facilitating tissue development and repair processes.

Pathways

TSPEAR plays a role in processes like the Notch signaling and Wnt signaling pathways. In the Notch signaling pathway it interacts with proteins involved in cell fate determinations assisting in the regulation of cell differentiation. Within the Wnt pathway TSPEAR associates with other proteins to influence cell growth and development. Its interaction with these pathways implies a cooperative role with proteins such as Notch receptors and beta-catenin highlighting its involvement in critical cellular communication networks.

TSPEAR has been linked to conditions like Alagille syndrome and certain skin disorders. In Alagille syndrome it interacts with proteins such as JAG1 and NOTCH2 which are important for the proper signaling required in fetal development. Moreover its expression levels can alter in various dermatological conditions connecting TSPEAR to proteins involved in skin structure and repair. Understanding its role in these disorders may provide insights into therapeutic targets and interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a critical role in tooth and hair follicle morphogenesis through regulation of the Notch signaling pathway (PubMed : 27736875). May play a role in development or function of the auditory system (PubMed : 22678063).
See full target information TSPEAR
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Chinese medical journal 137:1105-1114 PubMed37640670

2023

Effects of COL1A1 and SYTL2 on inflammatory cell infiltration and poor extracellular matrix remodeling of the vascular wall in thoracic aortic aneurysm.

Applications

Unspecified application

Species

Unspecified reactive species

Xinsheng Xie,Ye Yuan,Yulong Huang,Xiang Hong,Shichai Hong,Gang Chen,Yihui Chen,Yue Lin,Weifeng Lu,Weiguo Fu,Lixin Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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