Anti-TSPO antibody [12B8]

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TSPO antibody [12B8] (AB326712)

4% Paraformaldehyde-fixed Hela (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TSPO) monoclonal Antibody, unconjugated (ab326712) 1 : 100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, FITC) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Liver Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Prostate Tumor; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Prostate; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• Flow Cyt

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Flow Cytometry - Anti-TSPO antibody [12B8] (AB326712)

The Hela (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20°C, the cells then were incubated in 5% BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green) : Rabbit Anti-TSPO antibody (ab326712,1 : 100); Isotype Control (orange) : Rabbit IgG. Blank control (black) : PBS. Acquisition of 20,000 events was performed.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Lung Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Human Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Mouse Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Mouse Placenta; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Mouse Pancreas; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPO antibody [12B8] (AB326712)

Paraformaldehyde-fixed, paraffin embedded Mouse Stomach; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with TSPO Monoclonal Antibody, Unconjugated (ab326712) at 1 : 200 overnight at 4°C, followed by conjugation to the Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated and DAB staining.

• WB

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Western blot - Anti-TSPO antibody [12B8] (AB326712)

25 ug total protein per lane of various lysates (see on figure) probed with TSPO monoclonal antibody, unconjugated (ab326712) at 1 : 2000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

All lanes:

Western blot - Anti-TSPO antibody [12B8] (ab326712) at 1/2000 dilution

Lane 1:

RAW264.7 at 25 µg

Lane 2:

Mouse stomach at 25 µg

Lane 3:

HeLa at 25 µg

Lane 4:

293T at 25 µg

Lane 5:

MOLT-4 at 25 µg

false