Rabbit Polyclonal TSPY1/TSPY antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human TSPY1.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TSPY1. The exact immunogen used to generate this antibody is proprietary information. Database link Q01534
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.50000-1.00000 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
May be involved in sperm differentiation and proliferation.
Alternative names
TSPY, TSPY1, Testis-specific Y-encoded protein 1, Cancer/testis antigen 78, CT78
Recommended products
Rabbit Polyclonal TSPY1/TSPY antibody. C-terminal. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human TSPY1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TSPY1. The exact immunogen used to generate this antibody is proprietary information. Database link Q01534
- Purification technique
- Affinity purification Immunogen
- Specificity
At least three isoforms of TSPY1 are known to exist; this antibody will detect only TSPY1L (Isoform 2).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TSPY1 also known as Testis-Specific Protein Y-Encoded is a Y-linked gene with a protein product weighing approximately 38 kDa. This protein is mainly expressed in male germ cells found in the testes. TSPY1 functions as a nucleosome assembly factor which implicates its role in chromatin remodeling and regulation of transcription. It shows specific expression in the testis indicating its role in spermatogenesis and fertility processes.
- Biological function summary
TSPY1 influences cellular division and differentiation within the testes. As part of the DNA-binding protein family it associates with other proteins to impact germ cell progression. TSPY1 plays a role in the maintenance and proliferation of spermatogonial cells which are precursors to sperm cells. Its involvement here links it closely with cell cycle regulation and meiotic processes.
- Pathways
TSPY1 associates with regulatory processes important for the maintenance of spermatogonial stem cells. It participates in pathways including the Wnt signaling pathway which is essential for cell growth and differentiation. TSPY1 interacts with proteins such as Cyclin B influencing the G2/M transition during cell division. This interaction highlights its importance in ensuring proper cell cycle progression and indicates its pivotal role in reproductive biology.
- Associated diseases and disorders
TSPY1 has associations with testicular germ cell tumors and infertility. Abnormal expression of TSPY1 is found in a significant number of testicular cancer cases highlighting its oncogenic potential. It connects with oncogenes such as Cyclin D2 contributing to tumor cell proliferation. Furthermore deletions or duplications of TSPY1 might relate to spermatogenic failure causing male infertility. These associations highlight the importance of TSPY1 in both normal testicular function and its relevance in pathophysiological conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-TSPY1/TSPY antibody - C-terminal (ab189962)
Lane 1: Western blot - Anti-TSPY1/TSPY antibody - C-terminal (ab189962) at 0.5 µg/mL
Lane 2: Western blot - Anti-TSPY1/TSPY antibody - C-terminal (ab189962) at 1 µg/mL
All lanes: A20 cell lysate
Predicted band size: 35 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSPY1/TSPY antibody - C-terminal (ab189962)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling TSPY1/TSPY with ab189962 at 5μg/ml.
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