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AB251813

Anti-TSSC1 antibody

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Rabbit Polyclonal TSSC1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human EIPR1 aa 1-100.

View Alternative Names

TSSC1, EIPR1, EARP and GARP complex-interacting protein 1, Endosome-associated recycling protein-interacting protein, Golgi-associated retrograde protein-interacting protein, Tumor-suppressing STF cDNA 1 protein, Tumor-suppressing subchromosomal transferable fragment candidate gene 1 protein

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSC1 antibody (AB251813)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSC1 antibody (AB251813)

Paraffin-embedded human cerebral cortex tissue stained for TSSC1 using ab251813 at 1/20 dilution in immunohistochemical analysis.

Western blot - Anti-TSSC1 antibody (AB251813)
  • WB

Unknown

Western blot - Anti-TSSC1 antibody (AB251813)

All lanes:

Western blot - Anti-TSSC1 antibody (ab251813) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 2:

HEK-293T overexpressing human TSSC1 with a C-terminal myc-DDK tag, whole cell lysate

Predicted band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human EIPR1 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q53HC9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TSSC1 also known as Tumor Suppressor Candidate 1 plays a role in cellular machinery by facilitating protein complex formation. This protein has a molecular mass of approximately 47 kDa. Researchers find its expression in a wide range of tissues notably in highly metabolic active tissues. TSSC1's function involves maintaining structural integrity within the cell by aiding the assembly of multi-protein complexes which is essential for proper cellular operations.
Biological function summary

TSSC1 contributes to cellular homeostasis and development. It is part of the Commander complex which is important for endosomal sorting and trafficking. By aiding the assembly and stabilization of this complex TSSC1 supports cellular processes that rely on proper endosomal transport. This function maintains cellular activities that involve the movement and sorting of various biomolecules impacting growth and response mechanisms within cells.

Pathways

TSSC1 engages in intracellular transport and signal transduction pathways. It plays a significant role in the endosomal sorting pathway which controls material transport within cells. TSSC1 works closely with proteins like VPS26 and COMMD proteins which are essential players in endosomal sorting complexes. These interactions ensure efficient intracellular communication and materials handling affecting signal transduction pathways that mediate responses to external and internal signals.

Mutations and dysregulation of TSSC1 link to certain developmental disorders. Aberrant functioning of the endosomal sorting and trafficking process can lead to neurodevelopmental disorders due to its important role in neuronal growth and maintenance. Additionally TSSC1 may relate to cancer where its interaction with tumor suppressor pathways is altered. Disruptions in associated pathways or with proteins like TRIM25 which is involved in cellular stress responses can contribute to cancer development due to loss of regulatory control over cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a component of endosomal retrieval machinery that is involved in protein transport from early endosomes to either recycling endosomes or the trans-Golgi network (PubMed : 27440922). Mediates the recruitment of Golgi-associated retrograde protein (GARP) complex to the trans-Golgi network and controls early endosome-to-Golgi transport of internalized protein (PubMed : 27440922). Promotes the recycling of internalized transferrin receptor (TFRC) to the plasma membrane through interaction with endosome-associated recycling protein (EARP) complex (PubMed : 27440922). Controls proper insulin distribution and secretion, and retention of cargo in mature dense core vesicles (By similarity). Required for the stability of the endosome-associated retrograde protein (EARP) complex subunits and for proper localization and association of EARP with membranes (By similarity).
See full target information EIPR1
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Product promise

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