Rabbit Recombinant Monoclonal TSSK2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Images
![Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--western-blot-img94626.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108)")
![Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--western-blot-img124560.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120564.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (AB251108)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
| Rat | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Mouse | Dilution info - | Notes - |
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Testis-specific serine/threonine-protein kinase required during spermatid development. Phosphorylates TSKS at 'Ser-288' and SPAG16. Involved in the late stages of spermatogenesis, during the reconstruction of the cytoplasm. During spermatogenesis, required for the transformation of a ring-shaped structure around the base of the flagellum originating from the chromatoid body.
Alternative names
DGSG, SPOGA2, STK22B, TSSK2, Testis-specific serine/threonine-protein kinase 2, TSK-2, TSK2, TSSK-2, Testis-specific kinase 2, DiGeorge syndrome protein G, Serine/threonine-protein kinase 22B, DGS-G
Recommended products
Rabbit Recombinant Monoclonal TSSK2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR16116
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab251108 is the carrier-free version of Anti-TSSK2 antibody [EPR16116] ab192026.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Testis-specific serine/threonine kinase 2 commonly known as TSSK2 is a serine/threonine protein kinase with a molecular weight of approximately 42 kDa. TSSK2 is predominantly expressed in testicular tissue where it plays an essential role in sperm development. The protein is characterized by a kinase domain that is important for its activity allowing it to transfer a phosphate group from ATP to serine/threonine residues on substrate proteins which affects their function and activity.
- Biological function summary
The function of TSSK2 extends to supporting the process of spermatogenesis. It actively participates in sperm differentiation and maturation. TSSK2 does not typically form part of any larger protein complex but acts as an individual molecule with indispensable roles in sperm cell structure formation. Through its kinase activity it modifies other proteins critical for the development of functional sperm indicating its significant contribution to male fertility.
- Pathways
TSSK2 engages in the meiosis and spermatogenesis pathways serving a purpose in cellular processes that are fundamental for male germ cell development. It interacts with proteins such as HSP90 a chaperone protein which assists in the folding and function of various protein kinases during sperm production. TSSK2's actions within these pathways highlight its functional relevance and linkage to essential reproductive processes.
- Associated diseases and disorders
TSSK2 shows known associations with male infertility particularly cases characterized by failure in sperm production or maturation. Its malfunction or deficiency can compromise spermatogenesis which may relate back to defective kinase activity. Furthermore TSSK2 interplays with other proteins such as CK2 another kinase which also impacts fertility highlighting the broader network of interactions in which TSSK2 participates. Understanding these associations provides insight into potential therapeutic targets for infertility.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--western-blot-img94626.jpg "Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)")
Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)
This data was developed using Anti-TSSK2 antibody [EPR16116] ab192026, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TSSK2 antibody [EPR16116] (Anti-TSSK2 antibody [EPR16116] ab192026) at 1/1000 dilution
All lanes: Human testis lysate at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
![Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--western-blot-img124560.jpg "Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)")
Western blot - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)
This data was developed using Anti-TSSK2 antibody [EPR16116] ab192026, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TSSK2 antibody [EPR16116] (Anti-TSSK2 antibody [EPR16116] ab192026) at 1/1000 dilution
Lane 1: Mouse testis lysate at 20 µg
Lane 2: Rat testis lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108), expandable thumbnail](https://content.abcam.com/products/images/tssk2-antibody-epr16116-bsa-and-azide-free-ab251108--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img120564.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSSK2 antibody [EPR16116] - BSA and Azide free (ab251108)
This data was developed using Anti-TSSK2 antibody [EPR16116] ab192026, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TSSK2 with Anti-TSSK2 antibody [EPR16116] ab192026 at 1/1000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. The negative control utilised PBS instead of primary antibody. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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