AB231248

Anti-TST antibody

Name: Anti-TST antibody (ab231248) | Abcam
Brand: Abcam Limited
SKU: AB231248

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(5 Publications)

Rabbit Polyclonal THTR antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Mouse Thiosulfate sulfurtransferase.

View Alternative Names

Thiosulfate sulfurtransferase, Rhodanese, TST

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody (AB231248)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody (AB231248)

Formalin-fixed, paraffin-embedded mouse stomach tissue stained for TST with ab231248 at 20 μg/ml in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody (AB231248)

Formalin-fixed, paraffin-embedded mouse kidney tissue stained for TST with ab231248 at 20 μg/ml in immunohistochemical analysis. DAB staining.

Supplier Data

Western blot - Anti-TST antibody (AB231248)

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Western blot - Anti-TST antibody (ab231248) at 1/500 dilution

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Mouse kidney lysate

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-TST antibody (AB231248)

All lanes:

Western blot - Anti-TST antibody (ab231248) at 1/500 dilution

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Mouse liver lysate

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-TST antibody (AB231248)

All lanes:

Western blot - Anti-TST antibody (ab231248) at 1/500 dilution

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HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

Predicted band size: 33 kDa

false

Supplier Data

Western blot - Anti-TST antibody (AB231248)

All lanes:

Western blot - Anti-TST antibody (ab231248) at 1/500 dilution

All lanes:

Recombinant mouse TST protein

Predicted band size: 33 kDa,37 kDa,44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Mouse Thiosulfate sulfurtransferase.

P52196

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/400", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/400", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TST known as thiosulfate sulfurtransferase or rhodanese is an important enzyme involved in the detoxification of cyanide. It facilitates the transfer of sulfur to cyanide converting it into less harmful thiocyanate which the body can excrete. The enzyme has a molecular mass of approximately 33 kDa. It is highly expressed in the liver kidney and mitochondria of cells making it an important player in cellular detoxification processes.

Biological function summary

TST is important for maintaining sulfur metabolism and cyanide detoxification in the body. The enzyme is part of a multi-component system that helps neutralizing potentially toxic substances. By acting on substrates such as thiosulfate and cyanide TST forms an important partnership with other sulfurtransferases to support various cellular functions. Through its enzymatic actions TST contributes to oxidative stress regulation and cellular homeostasis.

Pathways

TST holds a significant position in the sulfur metabolism and detoxification pathways. It interacts with key proteins in these pathways like cysteine and methionine impacting amino acid metabolism. TST's function aligns with the mitochondrial respiratory chain helping to manage oxidative stress. Its activity also influences the levels of hydrogen sulfide a well-known signaling molecule linking TST to broader cellular activities involving oxidative stress responses.

TST has connections to conditions such as cyanide poisoning and hereditary rhodanese deficiency. Abnormal TST activity can disrupt normal detoxification and lead to metabolic imbalances affecting cellular health. Moreover insufficient TST function may associate with mitochondrial disorders given its close relationship with mitochondrial activity. This disruption in function can also correlate with oxidative stress leading to further cellular damage and health complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

See full target information Thiosulfate sulfurtransferase

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:1311 PubMed40883562

2025

Deficiency of KMT2D causes autistic-like behavior in mice and zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Huakun Shangguan,Jingguo Huang,Xia Wei,Jie Huang,Zaicheng Cao,Xingjingyan Chen,Jiahui Yuan,Jingrun Zhang,Binbin Ye,Ruofei Yan,Ruimin Chen,Wucheng Tao

Antioxidants (Basel, Switzerland) 12: PubMed36978895

2023

Role of Cystathionine β-Synthase and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics of Murine Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sidneia Sousa Santos,Larissa de Oliveira Cavalcanti Peres Rodrigues,Vanessa Martins,Maria Petrosino,Karim Zuhra,Kelly Ascenção,Abhishek Anand,Reham Mahmoud Abdel-Kader,Mohamed Z Gad,Carole Bourquin,Csaba Szabo

Antioxidants (Basel, Switzerland) 11: PubMed36139896

2022

Sequential Accumulation of 'Driver' Pathway Mutations Induces the Upregulation of Hydrogen-Sulfide-Producing Enzymes in Human Colonic Epithelial Cell Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly Ascenção,Nahzli Dilek,Karim Zuhra,Katalin Módis,Toshiro Sato,Csaba Szabo

GeroScience 44:2271-2289 PubMed35680713

2022

Inhibition of the 3-mercaptopyruvate sulfurtransferase-hydrogen sulfide system promotes cellular lipid accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Giovanna Casili,Elisa Randi,Theodora Panagaki,Karim Zuhra,Maria Petrosino,Csaba Szabo

Biomolecules 10: PubMed32183148

2020

Role of 3-Mercaptopyruvate Sulfurtransferase in the Regulation of Proliferation, Migration, and Bioenergetics in Murine Colon Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Fiona Augsburger,Elisa B Randi,Mathieu Jendly,Kelly Ascencao,Nahzli Dilek,Csaba Szabo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TST antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody (AB231248)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody (AB231248)

Western blot - Anti-TST antibody (AB231248)

Western blot - Anti-TST antibody (AB231248)

Western blot - Anti-TST antibody (AB231248)

Western blot - Anti-TST antibody (AB231248)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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