AB168359

Anti-TST antibody [EPR11645(B)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal THTR antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Thiosulfate sulfurtransferase, Rhodanese, TST

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11645(B)] (AB168359)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11645(B)] (AB168359)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling TST with ab168359 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11645(B)] (AB168359)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TST with ab168359 at 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TST antibody [EPR11645(B)] (AB168359)

All lanes:

Western blot - Anti-TST antibody [EPR11645(B)] (ab168359) at 1/1000 dilution

Lane 1:

Human fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 33 kDa

false

Carrier free

Anti-TST antibody [EPR11645(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11645(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TST known as thiosulfate sulfurtransferase or rhodanese is an important enzyme involved in the detoxification of cyanide. It facilitates the transfer of sulfur to cyanide converting it into less harmful thiocyanate which the body can excrete. The enzyme has a molecular mass of approximately 33 kDa. It is highly expressed in the liver kidney and mitochondria of cells making it an important player in cellular detoxification processes.

Biological function summary

TST is important for maintaining sulfur metabolism and cyanide detoxification in the body. The enzyme is part of a multi-component system that helps neutralizing potentially toxic substances. By acting on substrates such as thiosulfate and cyanide TST forms an important partnership with other sulfurtransferases to support various cellular functions. Through its enzymatic actions TST contributes to oxidative stress regulation and cellular homeostasis.

Pathways

TST holds a significant position in the sulfur metabolism and detoxification pathways. It interacts with key proteins in these pathways like cysteine and methionine impacting amino acid metabolism. TST's function aligns with the mitochondrial respiratory chain helping to manage oxidative stress. Its activity also influences the levels of hydrogen sulfide a well-known signaling molecule linking TST to broader cellular activities involving oxidative stress responses.

TST has connections to conditions such as cyanide poisoning and hereditary rhodanese deficiency. Abnormal TST activity can disrupt normal detoxification and lead to metabolic imbalances affecting cellular health. Moreover insufficient TST function may associate with mitochondrial disorders given its close relationship with mitochondrial activity. This disruption in function can also correlate with oxidative stress leading to further cellular damage and health complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

See full target information Thiosulfate sulfurtransferase

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical proteomics 13:26 PubMed27708557

2016

Plasma degradome affected by variable storage of human blood.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Kaisar,Leon F A van Dullemen,Marie-Laëtitia Thézénas,M Zeeshan Akhtar,Honglei Huang,Sandrine Rendel,Philip D Charles,Roman Fischer,Rutger J Ploeg,Benedikt M Kessler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TST antibody [EPR11645(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11645(B)] (AB168359)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11645(B)] (AB168359)

Western blot - Anti-TST antibody [EPR11645(B)] (AB168359)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise