AB166625

Anti-TST antibody [EPR11646(B)]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal THTR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

Thiosulfate sulfurtransferase, Rhodanese, TST

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

ab166625 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

ab166625 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (AB166625)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

ab166625 showing +ve staining in Human thyroid gland carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

ab166625 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TST antibody [EPR11646(B)] (AB166625)

All lanes:

Western blot - Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from fetal liver lysate at 10 µg

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-TST antibody [EPR11646(B)] (AB166625)

All lanes:

Western blot - Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 30 kDa,33 kDa

false

Carrier free

Anti-TST antibody [EPR11646(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11646(B)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TST known as thiosulfate sulfurtransferase or rhodanese is an important enzyme involved in the detoxification of cyanide. It facilitates the transfer of sulfur to cyanide converting it into less harmful thiocyanate which the body can excrete. The enzyme has a molecular mass of approximately 33 kDa. It is highly expressed in the liver kidney and mitochondria of cells making it an important player in cellular detoxification processes.

Biological function summary

TST is important for maintaining sulfur metabolism and cyanide detoxification in the body. The enzyme is part of a multi-component system that helps neutralizing potentially toxic substances. By acting on substrates such as thiosulfate and cyanide TST forms an important partnership with other sulfurtransferases to support various cellular functions. Through its enzymatic actions TST contributes to oxidative stress regulation and cellular homeostasis.

Pathways

TST holds a significant position in the sulfur metabolism and detoxification pathways. It interacts with key proteins in these pathways like cysteine and methionine impacting amino acid metabolism. TST's function aligns with the mitochondrial respiratory chain helping to manage oxidative stress. Its activity also influences the levels of hydrogen sulfide a well-known signaling molecule linking TST to broader cellular activities involving oxidative stress responses.

TST has connections to conditions such as cyanide poisoning and hereditary rhodanese deficiency. Abnormal TST activity can disrupt normal detoxification and lead to metabolic imbalances affecting cellular health. Moreover insufficient TST function may associate with mitochondrial disorders given its close relationship with mitochondrial activity. This disruption in function can also correlate with oxidative stress leading to further cellular damage and health complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

See full target information Thiosulfate sulfurtransferase

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 60:102629 PubMed36780769

2023

Hydrogen sulfide as an anti-calcification stratagem in human aortic valve: Altered biogenesis and mitochondrial metabolism of HS lead to HS deficiency in calcific aortic valve disease.

Applications

Unspecified application

Species

Unspecified reactive species

Zsolt Combi,László Potor,Péter Nagy,Katalin Éva Sikura,Tamás Ditrói,Eszter Petra Jurányi,Klaudia Galambos,Tamás Szerafin,Péter Gergely,Matthew Whiteman,Roberta Torregrossa,Yuchao Ding,Lívia Beke,Zoltán Hendrik,Gábor Méhes,György Balla,József Balla

Science immunology 7:eabq4531 PubMed36399538

2022

mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand .

Applications

Unspecified application

Species

Unspecified reactive species

Leslie Naesens,Santoshi Muppala,Dhiraj Acharya,Josephine Nemegeer,Delfien Bogaert,Jung-Hyun Lee,Katrien Staes,Veronique Debacker,Pieter De Bleser,Marieke De Bruyne,Elfride De Baere,Michiel van Gent,GuanQun Liu,Bart N Lambrecht,Jens Staal,Tessa Kerre,Rudi Beyaert,Jonathan Maelfait,Simon J Tavernier,Michaela U Gack,Filomeen Haerynck

Molecular carcinogenesis 62:185-199 PubMed36250643

2022

Metabolic-associated signature and hub genes associated with immune microenvironment and prognosis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Guo,Zongtai Zheng,Shiyu Mao,Fuhan Yang,Ruiliang Wang,Hong Wang,Ji Liu,Cheng Li,Qinwan Wang,Wentao Zhang,Xudong Yao,Shenghua Liu

Redox biology 51:102233 PubMed35042677

2022

Overproduction of hydrogen sulfide, generated by cystathionine β-synthase, disrupts brain wave patterns and contributes to neurobehavioral dysfunction in a rat model of down syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Theodora Panagaki,Laura Lozano-Montes,Lucia Janickova,Karim Zuhra,Marcell P Szabo,Tomas Majtan,Gregor Rainer,Damien Maréchal,Yann Herault,Csaba Szabo

Biomolecules 10: PubMed33352938

2020

Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Theodora Panagaki,Elisa B Randi,Csaba Szabo

Redox biology 38:101800 PubMed33271457

2020

The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and HS metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Anna-Theresa Mellis,Albert L Misko,Sita Arjune,Ye Liang,Katalin Erdélyi,Tamás Ditrói,Alexander T Kaczmarek,Peter Nagy,Guenter Schwarz

Journal of cellular and molecular medicine 24:12131-12140 PubMed32935914

2020

Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Elena Uyy,Viorel Iulian Suica,Raluca Maria Boteanu,Florentina Safciuc,Aurel Cerveanu-Hogas,Luminita Ivan,Crina Stavaru,Maya Simionescu,Felicia Antohe

International journal of molecular sciences 21: PubMed32012740

2020

Increased Urinary 3-Mercaptolactate Excretion and Enhanced Passive Systemic Anaphylaxis in Mice Lacking Mercaptopyruvate Sulfurtransferase, a Model of Mercaptolactate-Cysteine Disulfiduria.

Applications

Unspecified application

Species

Unspecified reactive species

Noriyuki Akahoshi,Tatsuro Minakawa,Masashi Miyashita,Uran Sugiyama,Chihiro Saito,Rintaro Takemoto,Akihiro Honda,Waka Kamichatani,Shotaro Kamata,Yasumi Anan,Isao Ishii

Molecular cell 72:1021-1034.e4 PubMed30472193

2018

Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Yaoyao Zhang,Zhiqiang Yan,Qingyuan Qin,Vicki Nisenblat,Hsun-Ming Chang,Yang Yu,Tianren Wang,Cuiling Lu,Ming Yang,Shuo Yang,Ying Yao,Xiaohui Zhu,Xi Xia,Yujiao Dang,Yixin Ren,Peng Yuan,Rong Li,Ping Liu,Hongyan Guo,Jinsong Han,Haojie He,Kun Zhang,Yiting Wang,Yu Wu,Meng Li,Jie Qiao,Jie Yan,Liying Yan

Oncotarget 8:104508-104524 PubMed29262657

2017

hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC.

Applications

Unspecified application

Species

Unspecified reactive species

Shitong Zhang,Jianjun Jin,Xiaoxiao Tian,Lijuan Wu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TST antibody [EPR11646(B)]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)

Western blot - Anti-TST antibody [EPR11646(B)] (AB166625)

Western blot - Anti-TST antibody [EPR11646(B)] (AB166625)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise