Rabbit Recombinant Monoclonal THTR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications.
Images
![Western blot - Anti-TST antibody [EPR11646(B)] (AB166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--western-blot-img70893.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TST antibody [EPR11646(B)] (AB166625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114813.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125933.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135142.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (AB166625)")
Publications
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- Redox biology 60:1026292023Hydrogen sulfide as an anti-calcification stratagem in human aortic valve: Altered biogenesis and mitochondrial metabolism of HS lead to HS deficiency in calcific aortic valve disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZsolt Combi,László Potor,Péter Nagy,Katalin Éva Sikura,Tamás Ditrói,Eszter Petra Jurányi,Klaudia Galambos,Tamás Szerafin,Péter Gergely,Matthew Whiteman,Roberta Torregrossa,Yuchao Ding,Lívia Beke,Zoltán Hendrik,Gábor Méhes,György Balla,József BallaPubMed 36780769
- Science immunology 7:eabq45312022mutations predispose to herpes simplex encephalitis by disrupting biogenesis of the host-derived RIG-I ligand .Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeslie Naesens,Santoshi Muppala,Dhiraj Acharya,Josephine Nemegeer,Delfien Bogaert,Jung-Hyun Lee,Katrien Staes,Veronique Debacker,Pieter De Bleser,Marieke De Bruyne,Elfride De Baere,Michiel van Gent,GuanQun Liu,Bart N Lambrecht,Jens Staal,Tessa Kerre,Rudi Beyaert,Jonathan Maelfait,Simon J Tavernier,Michaela U Gack,Filomeen HaerynckPubMed 36399538
- Molecular carcinogenesis 62:185-1992022Metabolic-associated signature and hub genes associated with immune microenvironment and prognosis in bladder cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYadong Guo,Zongtai Zheng,Shiyu Mao,Fuhan Yang,Ruiliang Wang,Hong Wang,Ji Liu,Cheng Li,Qinwan Wang,Wentao Zhang,Xudong Yao,Shenghua LiuPubMed 36250643
- Redox biology 51:1022332022Overproduction of hydrogen sulfide, generated by cystathionine β-synthase, disrupts brain wave patterns and contributes to neurobehavioral dysfunction in a rat model of down syndrome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheodora Panagaki,Laura Lozano-Montes,Lucia Janickova,Karim Zuhra,Marcell P Szabo,Tomas Majtan,Gregor Rainer,Damien Maréchal,Yann Herault,Csaba SzaboPubMed 35042677
- Biomolecules 10:2020Role of Hydrogen Sulfide and 3-Mercaptopyruvate Sulfurtransferase in the Regulation of the Endoplasmic Reticulum Stress Response in Hepatocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTheodora Panagaki et. al.PubMed 33352938
- Redox biology 38:1018002020The role of glutamate oxaloacetate transaminases in sulfite biosynthesis and HS metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna-Theresa Mellis,Albert L Misko,Sita Arjune,Ye Liang,Katalin Erdélyi,Tamás Ditrói,Alexander T Kaczmarek,Peter Nagy,Guenter SchwarzPubMed 33271457
- Journal of cellular and molecular medicine 24:12131-121402020Diabetic nephropathy associates with deregulation of enzymes involved in kidney sulphur metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElena Uyy et. al.PubMed 32935914
- International journal of molecular sciences 21:2020Increased Urinary 3-Mercaptolactate Excretion and Enhanced Passive Systemic Anaphylaxis in Mice Lacking Mercaptopyruvate Sulfurtransferase, a Model of Mercaptolactate-Cysteine Disulfiduria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoriyuki Akahoshi et. al.PubMed 32012740
- Molecular cell 72:1021-1034.e42018Transcriptome Landscape of Human Folliculogenesis Reveals Oocyte and Granulosa Cell Interactions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaoyao Zhang et. al.PubMed 30472193
- Oncotarget 8:104508-1045242017hsa-miR-29c-3p regulates biological function of colorectal cancer by targeting SPARC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShitong Zhang et. al.PubMed 29262657
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Tested |
| Mouse | Predicted | Not recommended | Predicted | Predicted |
| Rat | Predicted | Not recommended | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.
Alternative names
Thiosulfate sulfurtransferase, Rhodanese, TST
Recommended products
Rabbit Recombinant Monoclonal THTR antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11646(B)
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TST known as thiosulfate sulfurtransferase or rhodanese is an important enzyme involved in the detoxification of cyanide. It facilitates the transfer of sulfur to cyanide converting it into less harmful thiocyanate which the body can excrete. The enzyme has a molecular mass of approximately 33 kDa. It is highly expressed in the liver kidney and mitochondria of cells making it an important player in cellular detoxification processes.
- Biological function summary
TST is important for maintaining sulfur metabolism and cyanide detoxification in the body. The enzyme is part of a multi-component system that helps neutralizing potentially toxic substances. By acting on substrates such as thiosulfate and cyanide TST forms an important partnership with other sulfurtransferases to support various cellular functions. Through its enzymatic actions TST contributes to oxidative stress regulation and cellular homeostasis.
- Pathways
TST holds a significant position in the sulfur metabolism and detoxification pathways. It interacts with key proteins in these pathways like cysteine and methionine impacting amino acid metabolism. TST's function aligns with the mitochondrial respiratory chain helping to manage oxidative stress. Its activity also influences the levels of hydrogen sulfide a well-known signaling molecule linking TST to broader cellular activities involving oxidative stress responses.
- Associated diseases and disorders
TST has connections to conditions such as cyanide poisoning and hereditary rhodanese deficiency. Abnormal TST activity can disrupt normal detoxification and lead to metabolic imbalances affecting cellular health. Moreover insufficient TST function may associate with mitochondrial disorders given its close relationship with mitochondrial activity. This disruption in function can also correlate with oxidative stress leading to further cellular damage and health complications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Western blot - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--western-blot-img70893.jpg "Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)")
Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
All lanes: Western blot - Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution
Lane 1: Fetal liver lysate at 10 µg
Lane 2: HepG2 cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Predicted band size: 30 kDa, 33 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img114813.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
ab166625 showing +ve staining in Human thyroid gland carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135785.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
ab166625 showing +ve staining in Human normal prostate.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img125933.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
ab166625 showing +ve staining in Human normal kidney.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img135142.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
ab166625 showing +ve staining in Human breast carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41773.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131129.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)
Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunocytochemistry-immunofluorescence-img26996.jpg "Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)")
Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)
Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution.
![Western blot - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--western-blot-img87355.jpg "Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)")
Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)
All lanes: Western blot - Anti-TST antibody [EPR11646(B)] (ab166625) at 1/1000 dilution
All lanes: immunoprecipitation pellet from fetal liver lysate at 10 µg
Predicted band size: 33 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41773.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img131129.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] (ab166625)")
![Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--immunocytochemistry-immunofluorescence-img26996.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] (ab166625)")
![Western blot - Anti-TST antibody [EPR11646(B)] (ab166625), expandable thumbnail](https://content.abcam.com/products/images/tst-antibody-epr11646b-ab166625--western-blot-img87355.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TST antibody [EPR11646(B)] (ab166625)")
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