AB249363

Anti-TST antibody [EPR11646(B)] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal THTR antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Thiosulfate sulfurtransferase, Rhodanese, TST

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling TST with ab166625 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling TST with ab166625 at a 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

ab166625 showing +ve staining in Human breast carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

ab166625 showing +ve staining in Human normal kidney.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.Immunocytochemical analysis of HeLa cells labeling TST with ab166625 at a 1/100 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

ab166625 showing +ve staining in Human thyroid gland carcinoma.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

ab166625 showing +ve staining in Human normal prostate.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TST antibody [EPR11646(B)] (<a href='/en-us/products/primary-antibodies/tst-antibody-epr11646b-ab166625'>ab166625</a>) at 1/1000 dilution

All lanes:

immunoprecipitation pellet from fetal liver lysate at 10 µg

Predicted band size: 33 kDa

false

Unknown

Western blot - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

This data was developed using ab166625, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TST antibody [EPR11646(B)] (<a href='/en-us/products/primary-antibodies/tst-antibody-epr11646b-ab166625'>ab166625</a>) at 1/1000 dilution

Lane 1:

Fetal liver lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Predicted band size: 30 kDa,33 kDa

false

Unconjugated

Anti-TST antibody [EPR11646(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11646(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab249363 is the carrier-free version of ab166625.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TST known as thiosulfate sulfurtransferase or rhodanese is an important enzyme involved in the detoxification of cyanide. It facilitates the transfer of sulfur to cyanide converting it into less harmful thiocyanate which the body can excrete. The enzyme has a molecular mass of approximately 33 kDa. It is highly expressed in the liver kidney and mitochondria of cells making it an important player in cellular detoxification processes.

Biological function summary

TST is important for maintaining sulfur metabolism and cyanide detoxification in the body. The enzyme is part of a multi-component system that helps neutralizing potentially toxic substances. By acting on substrates such as thiosulfate and cyanide TST forms an important partnership with other sulfurtransferases to support various cellular functions. Through its enzymatic actions TST contributes to oxidative stress regulation and cellular homeostasis.

Pathways

TST holds a significant position in the sulfur metabolism and detoxification pathways. It interacts with key proteins in these pathways like cysteine and methionine impacting amino acid metabolism. TST's function aligns with the mitochondrial respiratory chain helping to manage oxidative stress. Its activity also influences the levels of hydrogen sulfide a well-known signaling molecule linking TST to broader cellular activities involving oxidative stress responses.

TST has connections to conditions such as cyanide poisoning and hereditary rhodanese deficiency. Abnormal TST activity can disrupt normal detoxification and lead to metabolic imbalances affecting cellular health. Moreover insufficient TST function may associate with mitochondrial disorders given its close relationship with mitochondrial activity. This disruption in function can also correlate with oxidative stress leading to further cellular damage and health complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Formation of iron-sulfur complexes, cyanide detoxification or modification of sulfur-containing enzymes. Other thiol compounds, besides cyanide, can act as sulfur ion acceptors. Also has weak mercaptopyruvate sulfurtransferase (MST) activity (By similarity). Together with MRPL18, acts as a mitochondrial import factor for the cytosolic 5S rRNA. Only the nascent unfolded cytoplasmic form is able to bind to the 5S rRNA.

See full target information Thiosulfate sulfurtransferase

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TST antibody [EPR11646(B)] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunocytochemistry/ Immunofluorescence - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Western blot - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Western blot - Anti-TST antibody [EPR11646(B)] - BSA and Azide free (AB249363)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise