AB190002

Anti-TSTA3 antibody

Name: Anti-TSTA3 antibody (ab190002) | Abcam
Brand: Abcam Limited
SKU: AB190002
Price: 630 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal TSTA3 antibody. Suitable for WB, IHC-P and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GFUS aa 200-300.

View Alternative Names

SDR4E1, TSTA3, GFUS, GDP-L-fucose synthase, Protein FX, Red cell NADP(H)-binding protein, Short-chain dehydrogenase/reductase family 4E member 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSTA3 antibody (AB190002)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSTA3 antibody (AB190002)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, labeling TSTA3 with ab190002 at 10 μg/ml.

Supplier Data

Western blot - Anti-TSTA3 antibody (AB190002)

All lanes:

Western blot - Anti-TSTA3 antibody (ab190002) at 1/500 dilution

Lane 1:

Rat muscle cell lysate

Lane 2:

Rat kidney cell lysate

Lane 3:

Rat muscle cell lysate with Immunizing peptide

Predicted band size: 36 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human GFUS aa 200-300. The exact immunogen used to generate this antibody is proprietary information.

Q13630

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trans-sialidase-related protein 3 (TSTA3) also known as GDP-L-fucose synthase is an enzyme responsible for converting GDP-4-keto-6-deoxy-D-mannose to GDP-L-fucose an important step in fucose synthesis. TSTA3 has a molecular mass of approximately 36 kDa. This protein expresses widely with significant expression in tissues like the liver which is a central site for metabolic processes.

Biological function summary

TSTA3 plays a role in fucosylation a fundamental process for the modification of glycoproteins and glycolipids. Fucosylation affects cellular recognition and signaling events. TSTA3 functions independently and as part of no known physical complex but influences several biological activities indirectly through its enzymatic product GDP-L-fucose. This product is critical in multiple cellular functions and structural carbohydrate assemblies.

Pathways

TSTA3 participates in the GDP-L-fucose biosynthesis pathway an essential route for the production of fucose necessary for fucosylation processes. This pathway ties directly to protein interactions involving glycosyltransferases such as fucosyltransferases which facilitate the transfer of fucose to glycoproteins and glycolipids. TSTA3's contribution to these pathways affects cell adhesion and immune response signaling.

TSTA3 relates mainly to cancers and congenital disorders of glycosylation. Abnormal fucosylation patterns often due to altered TSTA3 activity have been linked to various cancers impacting proteins like E-selectin which is involved in leukocyte adhesion. Additionally congenital disorders of glycosylation sometimes result from deficiencies in enzymes such as TSTA3 leading to disrupted glycan synthesis and presentation connecting to proteins such as glycoproteins with critical physiological roles.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the two-step NADP-dependent conversion of GDP-4-dehydro-6-deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction.

See full target information GFUS

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 23:285 PubMed37986192

2023

TSTA3 overexpression promotes malignant characteristics in LUSC by regulating LAMP2-mediated autophagy and tumor microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Yanlin Guo,Yanlong Hao,Liuyi Shen,Yu Du,Xiaohui Wang,Lvye Gao,Xuefei Feng,Yuanfang Zhai,Zhifei Liu,Enwei Xu,Yue Yang,Yanfeng Xi,Bin Yang,Ling Zhang

Oncology reports 45: PubMed33760213

2021

Tissue-specific transplantation antigen P35B functions as an oncogene and is regulated by microRNA-125a-5p in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yingjie Gao,Guangliang Zhang,Jinlong Liu,Huimin Li

Theranostics 10:11339-11358 PubMed33042286

2020

TSTA3 facilitates esophageal squamous cell carcinoma progression through regulating fucosylation of LAMP2 and ERBB2.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhang,Yingzhen Gao,Xiaojuan Zhang,Min Guo,Jie Yang,Heyang Cui,Pengzhou Kong,Xia Niu,Yanghui Bi,Jing Xu,Ting Yan,Yanchun Ma,Jian Yang,Yu Qian,Fang Wang,Hongyi Li,Feng Liu,Xiaolong Cheng,Yongping Cui

Technology in cancer research & treatment 17:1533033818781405 PubMed29950151

2018

High TSTA3 Expression as a Candidate Biomarker for Poor Prognosis of Patients With ESCC.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Yang,Pengzhou Kong,Jian Yang,Zhiwu Jia,Xiaoling Hu,Zianyi Wang,Heyang Cui,Yanghui Bi,Yu Qian,Hongyi Li,Fang Wang,Bin Yang,Ting Yan,Yanchun Ma,Ling Zhang,Caixia Cheng,Bin Song,Yaoping Li,Enwei Xu,Haiyan Liu,Wei Gao,Juan Wang,Yiqian Liu,Yuanfang Zhai,Lu Chang,Yi Wang,Yingchun Zhang,Ruyi Shi,Jing Liu,Qi Wang,Xiaolong Cheng,Yongping Cui

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TSTA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TSTA3 antibody (AB190002)

Western blot - Anti-TSTA3 antibody (AB190002)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise