Anti-TTC11/FIS1 antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (AB229969)

Paraffin-embedded human kidney tissue stained for TTC11/FIS1 using ab229969 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (AB229969)

Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TTC11/FIS1 (green) using ab229969 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488^® congugated Goat Anti-Rabbit IgG (H+L).

• IP

Supplier Data

Immunoprecipitation - Anti-TTC11/FIS1 antibody (AB229969)

TTC11/FIS1 was immunoprecipitated from 500 μg MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 4 μg of ab229969.

Lane 1 : Rabbit monoclonal IgG (1 μg) IP instead of ab229969 in MCF7 whole cell lysate.

Lane 2 : ab229969 IP in MCF whole cell lysate.

Lane 3 : MCF7 whole cell lysate 20 ug (input).

For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

All lanes:

Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969)

Predicted band size: 17 kDa

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• WB

Lab

Western blot - Anti-TTC11/FIS1 antibody (AB229969)

Western blot : Rabbit Polyclonal to TTC11/FIS1 229969 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 16 kDa in Wild-type Jurkat cell lysates with no signal observed at this size in FIS1 knockout Jurkat cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab229969) at 1/2000 dilution

Lane 1:

Wild-type Jurkat at 20 µg

Lane 2:

FIS1 knockout Jurkat at 20 µg

Lane 3:

HeLa at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 17 kDa

Observed band size: 16 kDa

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• WB

Supplier Data

Western blot - Anti-TTC11/FIS1 antibody (AB229969)

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab229969) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

Lane 2:

Mouse brain lysate

Lane 3:

Mouse heart lysate

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 17 kDa

Observed band size: 17 kDa

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