Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% PBS
Liquid
Polyclonal
IHC-P | IP | WB | ICC/IF | |
---|---|---|---|---|
Human | Tested | Tested | Tested | Tested |
Mouse | Expected | Expected | Tested | Expected |
Rat | Predicted | Predicted | Predicted | Predicted |
Cow | Predicted | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/200 - 1/2000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/5000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/50 - 1/500 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rat, Cow | Dilution info - | Notes - |
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Involved in the fragmentation of the mitochondrial network and its perinuclear clustering (PubMed:12783892, PubMed:12861026, PubMed:14996942, PubMed:23283981). Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission (PubMed:12861026, PubMed:16118244, PubMed:23283981, PubMed:23530241, PubMed:24196833). May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event (PubMed:23530241, PubMed:24196833). Also mediates peroxisomal fission (PubMed:16107562). May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis (PubMed:24196833). Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis (PubMed:12783892).
TTC11, CGI-135, FIS1, Mitochondrial fission 1 protein, FIS1 homolog, Tetratricopeptide repeat protein 11, hFis1, TPR repeat protein 11
Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.
IgG
Rabbit
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% PBS
Liquid
Polyclonal
Affinity purification Protein G
Purity >95%.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TTC11 also known as FIS1 is a protein that plays an important role in mitochondrial dynamics. It has a molecular weight of approximately 17 kDa. The FIS1 protein is expressed in various tissues suggesting it has a broad functional impact across different cell types. Through its structure it facilitates mitochondrial fission by interacting with other mitochondrial proteins. This process ensures proper distribution and health of mitochondria within the cell.
FIS1 is critical in controlling mitochondrial size and quantity. It is part of a complex that includes proteins like DRP1 which is heavily involved in promoting the division of mitochondria. By working with DRP1 FIS1 helps maintain cellular energy levels and dynamics by ensuring that mitochondria are appropriately divided and distributed. This role impacts overall cell health and function.
FIS1 is integral to the pathways regulating mitochondrial fission and fusion. It interacts closely within the pathway involving DRP1 and MFF both of which are critical in these processes. Furthermore FIS1's interactions in these pathways help balance mitochondrial fission and fusion maintaining cellular energy homeostasis and proper mitochondrial function.
Defects in FIS1 function relate to neurodegenerative diseases such as Alzheimer's disease and Charcot-Marie-Tooth disease. In these contexts abnormal interactions with DRP1 can lead to impaired mitochondrial dynamics contributing to disease progression. Research into these relationships continues to uncover the important role FIS1 plays in maintaining cellular health and its potential as a therapeutic target.
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All lanes: Western blot - Anti-TTC11/FIS1 antibody (ab229969) at 1/1000 dilution
Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2: Mouse brain lysate
Lane 3: Mouse heart lysate
All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDa
Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TTC11/FIS1 (green) using ab229969 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
TTC11/FIS1 was immunoprecipitated from 500 μg MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 4 μg of ab229969.
Lane 1: Rabbit monoclonal IgG (1 μg) IP instead of ab229969 in MCF7 whole cell lysate.
Lane 2: ab229969 IP in MCF whole cell lysate.
Lane 3: MCF7 whole cell lysate 20 ug (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
All lanes: Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969)
Predicted band size: 17 kDa
Paraffin-embedded human kidney tissue stained for TTC11/FIS1 using ab229969 at 1/600 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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