Skip to main content

Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.

Be the first to review this product! Submit a review

Images

Western blot - Anti-TTC11/FIS1 antibody (AB229969), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (AB229969), expandable thumbnail
  • Immunoprecipitation - Anti-TTC11/FIS1 antibody (AB229969), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (AB229969), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% PBS

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human FIS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3D6

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPWBICC/IF
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Rat
Predicted
Predicted
Predicted
Predicted
Cow
Predicted
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500 - 1/1000

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/200 - 1/2000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000 - 1/5000

Notes

-

Species

Mouse

Dilution info

1/1000 - 1/5000

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50 - 1/500

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

6 products for Alternative Product

Target data

Function

Involved in the fragmentation of the mitochondrial network and its perinuclear clustering (PubMed:12783892, PubMed:12861026, PubMed:14996942, PubMed:23283981). Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission (PubMed:12861026, PubMed:16118244, PubMed:23283981, PubMed:23530241, PubMed:24196833). May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event (PubMed:23530241, PubMed:24196833). Also mediates peroxisomal fission (PubMed:16107562). May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis (PubMed:24196833). Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis (PubMed:12783892).

Alternative names

Recommended products

Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for IHC-P, IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human FIS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3D6
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Purity >95%.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TTC11 also known as FIS1 is a protein that plays an important role in mitochondrial dynamics. It has a molecular weight of approximately 17 kDa. The FIS1 protein is expressed in various tissues suggesting it has a broad functional impact across different cell types. Through its structure it facilitates mitochondrial fission by interacting with other mitochondrial proteins. This process ensures proper distribution and health of mitochondria within the cell.

Biological function summary

FIS1 is critical in controlling mitochondrial size and quantity. It is part of a complex that includes proteins like DRP1 which is heavily involved in promoting the division of mitochondria. By working with DRP1 FIS1 helps maintain cellular energy levels and dynamics by ensuring that mitochondria are appropriately divided and distributed. This role impacts overall cell health and function.

Pathways

FIS1 is integral to the pathways regulating mitochondrial fission and fusion. It interacts closely within the pathway involving DRP1 and MFF both of which are critical in these processes. Furthermore FIS1's interactions in these pathways help balance mitochondrial fission and fusion maintaining cellular energy homeostasis and proper mitochondrial function.

Associated diseases and disorders

Defects in FIS1 function relate to neurodegenerative diseases such as Alzheimer's disease and Charcot-Marie-Tooth disease. In these contexts abnormal interactions with DRP1 can lead to impaired mitochondrial dynamics contributing to disease progression. Research into these relationships continues to uncover the important role FIS1 plays in maintaining cellular health and its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-TTC11/FIS1 antibody (ab229969), expandable thumbnail

    Western blot - Anti-TTC11/FIS1 antibody (ab229969)

    All lanes: Western blot - Anti-TTC11/FIS1 antibody (ab229969) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate

    Lane 2: Mouse brain lysate

    Lane 3: Mouse heart lysate

    Secondary

    All lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 17 kDa

    Observed band size: 17 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (ab229969), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (ab229969)

    Immunofluorescent analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TTC11/FIS1 (green) using ab229969 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969), expandable thumbnail

    Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969)

    TTC11/FIS1 was immunoprecipitated from 500 μg MCF7 (human breast adenocarcinoma cell line) whole cell lysate with 4 μg of ab229969.

    Lane 1: Rabbit monoclonal IgG (1 μg) IP instead of ab229969 in MCF7 whole cell lysate.

    Lane 2: ab229969 IP in MCF whole cell lysate.

    Lane 3: MCF7 whole cell lysate 20 ug (input).

    For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.

    All lanes: Immunoprecipitation - Anti-TTC11/FIS1 antibody (ab229969)

    Predicted band size: 17 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (ab229969), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (ab229969)

    Paraffin-embedded human kidney tissue stained for TTC11/FIS1 using ab229969 at 1/600 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com