Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.
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- International journal of nanomedicine 17:5431-54462022Activation of Dynamin-Related Protein 1 and Induction of Mitochondrial Apoptosis by Exosome-Rifampicin Nanoparticles Exerts Anti-Osteosarcoma Effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenkai Chen,Wenping Lin,Naichun Yu,Linlin Zhang,Zuoxing Wu,Yongjie Chen,Zongguang Li,Fengqing Gong,Na Li,Xiaohui Chen,Xu He,Yue Wu,Xiangchen Zeng,Yuting Yueh,Ren Xu,Guangrong JiPubMed 36426375
- International journal of molecular sciences 23:2022Mitochondrial Dysfunction in Spinocerebellar Ataxia Type 3 Is Linked to VDAC1 Deubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTina Harmuth,Jonasz J Weber,Anna J Zimmer,Anna S Sowa,Jana Schmidt,Julia C Fitzgerald,Ludger Schöls,Olaf Riess,Jeannette Hübener-SchmidPubMed 35682609
- Evidence-based complementary and alternative medicine : eCAM 2022:38494412022Electroacupuncture Attenuates Learning and Memory Impairment via PI3K/Akt Pathway in an Amyloid -Induced Alzheimer's Disease Mouse Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi-Mai Shao,Kyung Hye Park,Ye Yuan,Zijuan Zhang,Yanwen You,Zhenqiang Zhang,Li HaoPubMed 35463064
- The journal of physiological sciences : JPS 71:322021Shen Qi Li Xin formula improves chronic heart failure through balancing mitochondrial fission and fusion via upregulation of PGC-1α.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan-Bo Sui,Jian Xiu,Jin-Xuan Wei,Pei-Pei Pan,Bi-Hong Sun,Li LiuPubMed 34663205
- FEBS open bio 11:2836-28442021Lactate administration does not affect denervation-induced loss of mitochondrial content and muscle mass in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKenya Takahashi,Yu Kitaoka,Yutaka Matsunaga,Hideo HattaPubMed 34510821
- Physiological reports 9:e150162021Voluntary exercise prevents abnormal muscle mitochondrial morphology in cancer cachexia mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Kitaoka,Mitsunori Miyazaki,Shin KikuchiPubMed 34427401
- Cell death & disease 11:8882020Prevention of mitochondrial impairment by inhibition of protein phosphatase 1 activity in amyotrophic lateral sclerosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSo Yoen Choi et. al.PubMed 33087694
- Journal of molecular and cellular cardiology 146:109-1202020Mcl-1-mediated mitochondrial fission protects against stress but impairs cardiac adaptation to exercise.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexandra G Moyzis,Navraj S Lally,Wenjing Liang,Leonardo J Leon,Rita H Najor,Amabel M Orogo,Åsa B GustafssonPubMed 32717194
- Aging cell 19:e131872020Aging is associated with a decline in Atg9b-mediated autophagosome formation and appearance of enlarged mitochondria in the heart.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWenjing Liang et. al.PubMed 32627317
- Toxins 12:2020T-2 Toxin-Induced Oxidative Stress Leads to Imbalance of Mitochondrial Fission and Fusion to Activate Cellular Apoptosis in the Human Liver 7702 Cell Line.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunhua Yang et. al.PubMed 31936883
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human FIS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3D6
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
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Target data
Function
Involved in the fragmentation of the mitochondrial network and its perinuclear clustering (PubMed:12783892, PubMed:12861026, PubMed:14996942, PubMed:23283981). Plays a minor role in the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface and mitochondrial fission (PubMed:12861026, PubMed:16118244, PubMed:23283981, PubMed:23530241, PubMed:24196833). May not be essential for the assembly of functional fission complexes and the subsequent membrane scission event (PubMed:23530241, PubMed:24196833). Also mediates peroxisomal fission (PubMed:16107562). May act when the products of fission are directed toward mitochondrial homeostasis, mitophagy, or apoptosis (PubMed:24196833). Can induce cytochrome c release from the mitochondrion to the cytosol, ultimately leading to apoptosis (PubMed:12783892).
Alternative names
TTC11, CGI-135, FIS1, Mitochondrial fission 1 protein, FIS1 homolog, Tetratricopeptide repeat protein 11, hFis1, TPR repeat protein 11
Recommended products
Rabbit Polyclonal TTC11/FIS1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 21 publications. Immunogen corresponding to Recombinant Fragment Protein within Human FIS1 aa 1-150.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human FIS1 aa 1-150. The exact immunogen used to generate this antibody is proprietary information. Database link Q9Y3D6
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TTC11 also known as FIS1 is a protein that plays an important role in mitochondrial dynamics. It has a molecular weight of approximately 17 kDa. The FIS1 protein is expressed in various tissues suggesting it has a broad functional impact across different cell types. Through its structure it facilitates mitochondrial fission by interacting with other mitochondrial proteins. This process ensures proper distribution and health of mitochondria within the cell.
- Biological function summary
FIS1 is critical in controlling mitochondrial size and quantity. It is part of a complex that includes proteins like DRP1 which is heavily involved in promoting the division of mitochondria. By working with DRP1 FIS1 helps maintain cellular energy levels and dynamics by ensuring that mitochondria are appropriately divided and distributed. This role impacts overall cell health and function.
- Pathways
FIS1 is integral to the pathways regulating mitochondrial fission and fusion. It interacts closely within the pathway involving DRP1 and MFF both of which are critical in these processes. Furthermore FIS1's interactions in these pathways help balance mitochondrial fission and fusion maintaining cellular energy homeostasis and proper mitochondrial function.
- Associated diseases and disorders
Defects in FIS1 function relate to neurodegenerative diseases such as Alzheimer's disease and Charcot-Marie-Tooth disease. In these contexts abnormal interactions with DRP1 can lead to impaired mitochondrial dynamics contributing to disease progression. Research into these relationships continues to uncover the important role FIS1 plays in maintaining cellular health and its potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TTC11/FIS1 antibody (ab96764)
15% SDS-PAGE
All lanes: Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution
All lanes: Raji whole cell lysate at 30 µg
Predicted band size: 17 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (ab96764)
Immunohistochemical analysis of TTC11/FIS1 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (ab96764)
ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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