Anti-TTC11/FIS1 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (AB96764)

Immunofluorescence analysis of 2% paraformaldehyde/culture medium fixed at 37°C for 30 min HeLa cells labelling FIS1 protein at mitochondria using ab96764 at a 1/2000 dilution (Green). The nuclear counterstain is Hoechst 33342 (Blue). Scale bar= 10 µm.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC11/FIS1 antibody (AB96764)

Immunohistochemical analysis of TTC11/FIS1 in paraffin-embedded NCIN87 xenograft, using ab96764 antibody at 1/100 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TTC11/FIS1 antibody (AB96764)

ICC/IF image of ab96764 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab96764, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

Supplier Data

Western blot - Anti-TTC11/FIS1 antibody (AB96764)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

• WB

Unknown

Western blot - Anti-TTC11/FIS1 antibody (AB96764)

15% SDS-PAGE

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-TTC11/FIS1 antibody (AB96764)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/10000 dilution

Lane 1:

293T Whole cell extract at 30 µg

Lane 2:

A431 Whole cell extract at 30 µg

Lane 3:

HeLa Whole cell extract at 30 µg

Lane 4:

HepG2 Whole cell extract at 30 µg

Predicted band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-TTC11/FIS1 antibody (AB96764)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/5000 dilution

Lane 1:

Non-transfected (-) HeLa whole cell extracts at 30 µg

Lane 2:

FIS1 shRNA transfected (+) HeLa whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false

• WB

Supplier Data

Western blot - Anti-TTC11/FIS1 antibody (AB96764)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-TTC11/FIS1 antibody (ab96764) at 1/5000 dilution

All lanes:

Mouse liver tissue extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 17 kDa

false