AB181191

Anti-TTC19 antibody [EPR13184-63]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TTC19 antibody. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TPR repeat protein 19, TTC19

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (AB181191)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (AB181191)

Immunocytochemical analysis of HT-1080 cells fixed in 4% paraformaldehyde labeling TTC19 with ab181191 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution (green). Counterstained with DAP1 (blue)

Supplier Data

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (AB181191)

Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (ab181191)

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184-63] (AB181191)

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184-63] (ab181191) at 1/5000 dilution

Lane 1:

Human ovarian cancer lysate at 20 µg

Lane 2:

HT-1080 lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Carrier free

Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13184-63

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/20 - 1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC19 also known as Tetratricopeptide Repeat Domain 19 operates mechanically as a nuclear-encoded mitochondrial protein involved in mitoribosome assembly and function. This protein has a mass of approximately 45 kDa. Researchers have identified its presence mainly in mitochondria-rich tissues such as the brain and heart. The expression pattern suggests its significant role in tissues with high energy demand.

Biological function summary

This protein plays an important role in regulating mitochondrial respiratory chain complex III assembly. TTC19 facilitates the proper incorporation of the UQCRB subunit into complex III essential for the electron transport chain. It belongs to a multi-subunit complex within the inner mitochondrial membrane. This complex ensures optimal mitochondrial performance highlighting TTC19's role in cellular energy homeostasis.

Pathways

TTC19 influences mitochondrial and oxidative phosphorylation pathways. These pathways are critical for ATP production in cell metabolism. TTC19 interacts with proteins such as UQCRC1 during the electron transport process. By enabling efficient electron flow TTC19 supports cellular respiration and energy conversion pathways essential for cell viability.

Functional deficiencies or mutations in this target link to diseases like mitochondrial complex III deficiency and Leigh Syndrome. These conditions often manifest with neurological symptoms and multisystemic involvement. Proteins such as cytochrome c can become indirectly affected by TTC19 defects leading to compromised electron transport and cellular energy production ultimately contributing to disease etiology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed : 21278747, PubMed : 28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation of UQCRFS1 into the complex III and whose presence is detrimental for its catalytic activity (PubMed : 28673544).

See full target information TTC19

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed37159335

2023

Restoring calcium homeostasis in Purkinje cells arrests neurodegeneration and neuroinflammation in the ARSACS mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Del Bondio,Fabiana Longo,Daniele De Ritis,Erica Spirito,Paola Podini,Bernard Brais,Angela Bachi,Angelo Quattrini,Francesca Maltecca

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTC19 antibody [EPR13184-63]

Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184-63] (AB181191)

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] (AB181191)

Western blot - Anti-TTC19 antibody [EPR13184-63] (AB181191)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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