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AB250415

Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free

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Rabbit Recombinant Monoclonal TTC19 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

TPR repeat protein 19, TTC19

2 Images
Western blot - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (AB250415)
  • WB

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (AB250415)

This data was developed using ab181191, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (ab250415) at 1/5000 dilution

Lane 1:

Human ovarian cancer lysate at 20 µg

Lane 2:

HT-1080 lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (AB250415)
  • IP

Unknown

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (AB250415)

This data was developed using ab181191, the same antibody clone in a different buffer formulation.

Immunoprecipitation of HT-1080 cells labeling TTC19 with ab181191 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-TTC19 antibody [EPR13184-63] - BSA and Azide free (ab250415)

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13184-63

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250415 is the carrier-free version of ab181191.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC19 also known as Tetratricopeptide Repeat Domain 19 operates mechanically as a nuclear-encoded mitochondrial protein involved in mitoribosome assembly and function. This protein has a mass of approximately 45 kDa. Researchers have identified its presence mainly in mitochondria-rich tissues such as the brain and heart. The expression pattern suggests its significant role in tissues with high energy demand.
Biological function summary

This protein plays an important role in regulating mitochondrial respiratory chain complex III assembly. TTC19 facilitates the proper incorporation of the UQCRB subunit into complex III essential for the electron transport chain. It belongs to a multi-subunit complex within the inner mitochondrial membrane. This complex ensures optimal mitochondrial performance highlighting TTC19's role in cellular energy homeostasis.

Pathways

TTC19 influences mitochondrial and oxidative phosphorylation pathways. These pathways are critical for ATP production in cell metabolism. TTC19 interacts with proteins such as UQCRC1 during the electron transport process. By enabling efficient electron flow TTC19 supports cellular respiration and energy conversion pathways essential for cell viability.

Functional deficiencies or mutations in this target link to diseases like mitochondrial complex III deficiency and Leigh Syndrome. These conditions often manifest with neurological symptoms and multisystemic involvement. Proteins such as cytochrome c can become indirectly affected by TTC19 defects leading to compromised electron transport and cellular energy production ultimately contributing to disease etiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed : 21278747, PubMed : 28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation of UQCRFS1 into the complex III and whose presence is detrimental for its catalytic activity (PubMed : 28673544).
See full target information TTC19
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Product promise

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