JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB250323

Anti-TTC19 antibody [EPR13184] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal TTC19 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human samples.

View Alternative Names

TPR repeat protein 19, TTC19

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)

This data was developed using ab181069, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HT-1080 cells, staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)
  • IP

Supplier Data

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)

This data was developed using ab181069, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (<a href='/en-us/products/primary-antibodies/ttc19-antibody-epr13184-c-terminal-ab181069'>ab181069</a>)

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)
  • WB

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)

This data was developed using ab181069, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (<a href='/en-us/products/primary-antibodies/ttc19-antibody-epr13184-c-terminal-ab181069'>ab181069</a>) at 1/10000 dilution

All lanes:

HT-1080 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)
  • WB

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184] - BSA and Azide free (AB250323)

This data was developed using ab181069, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (<a href='/en-us/products/primary-antibodies/ttc19-antibody-epr13184-c-terminal-ab181069'>ab181069</a>) at 1/2000 dilution

Lane 1:

Human fetal muscle tissue lysate at 20 µg

Lane 2:

Human ovarian cancer tissue lysate at 20 µg

Lane 3:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13184

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB139191

Anti-CCDC98 antibody [EPR6310(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] (AB139191)

  • 0
  • 0
Primary Antibodies

AB204428

Anti-SUGT1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-SUGT1 antibody (AB204428)

  • 0
  • 0
Primary Antibodies

AB119994

Anti-Maltose Binding Protein antibody [EPR4744]

RabMAb|Recombinant|Lab Essentials

Western blot - Anti-Maltose Binding Protein antibody [EPR4744] (AB119994)

  • 0
  • 0
Primary Antibodies

AB154590

Anti-WBP11 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WBP11 antibody (AB154590)

  • 5
  • 2
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>The observed MW is lower than the predicted 42kDa, which may be due to the removing of 8KDa transit peptide.</p>" } } }
style
left-column

Product details

ab250323 is the carrier-free version of ab181069.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC19 also known as Tetratricopeptide Repeat Domain 19 operates mechanically as a nuclear-encoded mitochondrial protein involved in mitoribosome assembly and function. This protein has a mass of approximately 45 kDa. Researchers have identified its presence mainly in mitochondria-rich tissues such as the brain and heart. The expression pattern suggests its significant role in tissues with high energy demand.
Biological function summary

This protein plays an important role in regulating mitochondrial respiratory chain complex III assembly. TTC19 facilitates the proper incorporation of the UQCRB subunit into complex III essential for the electron transport chain. It belongs to a multi-subunit complex within the inner mitochondrial membrane. This complex ensures optimal mitochondrial performance highlighting TTC19's role in cellular energy homeostasis.

Pathways

TTC19 influences mitochondrial and oxidative phosphorylation pathways. These pathways are critical for ATP production in cell metabolism. TTC19 interacts with proteins such as UQCRC1 during the electron transport process. By enabling efficient electron flow TTC19 supports cellular respiration and energy conversion pathways essential for cell viability.

Functional deficiencies or mutations in this target link to diseases like mitochondrial complex III deficiency and Leigh Syndrome. These conditions often manifest with neurological symptoms and multisystemic involvement. Proteins such as cytochrome c can become indirectly affected by TTC19 defects leading to compromised electron transport and cellular energy production ultimately contributing to disease etiology.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed : 21278747, PubMed : 28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation of UQCRFS1 into the complex III and whose presence is detrimental for its catalytic activity (PubMed : 28673544).
See full target information TTC19
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com