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AB181069

Anti-TTC19 antibody [EPR13184] - C-terminal

5

(1 Review)

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(1 Publication)

Rabbit Recombinant Monoclonal TTC19 antibody. C-terminal. Suitable for IP, WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TPR repeat protein 19, TTC19

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)

Immunofluorescence analysis of HT-1080 cells staining TTC19 (green) with ab181069. Goat anti rabbit IgG (Alexa Fluor®488) was used as a secondary antibody at 1/200 dilution. Counterstained with DAPI (blue)

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)
  • IP

Supplier Data

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)

Western blot analysis on immunoprecipitation pellet from HT-1080, labeling TTC19 immunoprecipitated using ab181069 at 1/70 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069)

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)
  • WB

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/10000 dilution

All lanes:

HT-1080 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)
  • WB

Supplier Data

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (AB181069)

All lanes:

Western blot - Anti-TTC19 antibody [EPR13184] - C-terminal (ab181069) at 1/2000 dilution

Lane 1:

Human fetal muscle tissue lysate at 20 µg

Lane 2:

Human ovarian cancer tissue lysate at 20 µg

Lane 3:

Human fetal heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/1000 dilution

Predicted band size: 42 kDa

Observed band size: 35 kDa

false

  • Carrier free

    Anti-TTC19 antibody [EPR13184] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13184

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/70", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p>The observed MW is lower than the predicted 42kDa, which may be due to the removing of 8KDa transit peptide.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC19 also known as Tetratricopeptide Repeat Domain 19 operates mechanically as a nuclear-encoded mitochondrial protein involved in mitoribosome assembly and function. This protein has a mass of approximately 45 kDa. Researchers have identified its presence mainly in mitochondria-rich tissues such as the brain and heart. The expression pattern suggests its significant role in tissues with high energy demand.
Biological function summary

This protein plays an important role in regulating mitochondrial respiratory chain complex III assembly. TTC19 facilitates the proper incorporation of the UQCRB subunit into complex III essential for the electron transport chain. It belongs to a multi-subunit complex within the inner mitochondrial membrane. This complex ensures optimal mitochondrial performance highlighting TTC19's role in cellular energy homeostasis.

Pathways

TTC19 influences mitochondrial and oxidative phosphorylation pathways. These pathways are critical for ATP production in cell metabolism. TTC19 interacts with proteins such as UQCRC1 during the electron transport process. By enabling efficient electron flow TTC19 supports cellular respiration and energy conversion pathways essential for cell viability.

Functional deficiencies or mutations in this target link to diseases like mitochondrial complex III deficiency and Leigh Syndrome. These conditions often manifest with neurological symptoms and multisystemic involvement. Proteins such as cytochrome c can become indirectly affected by TTC19 defects leading to compromised electron transport and cellular energy production ultimately contributing to disease etiology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for the preservation of the structural and functional integrity of mitochondrial respiratory complex III by allowing the physiological turnover of the Rieske protein UQCRFS1 (PubMed : 21278747, PubMed : 28673544). Involved in the clearance of UQCRFS1 N-terminal fragments, which are produced upon incorporation of UQCRFS1 into the complex III and whose presence is detrimental for its catalytic activity (PubMed : 28673544).
See full target information TTC19
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Aging 12:9461-9474 PubMed32452827

2020

Regulation of PGC-1α mediated by acetylation and phosphorylation in MPP+ induced cell model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Fan,Songlin Li,Zhipeng Wen,Qiaoyue Ye,Xiaochun Chen,Qinyong Ye
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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