AB201229

Anti-TTC21A/STI2 antibody [EPR17437]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TTC21A/STI2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

STI2, TTC21A, Tetratricopeptide repeat protein 21A, TPR repeat protein 21A, Stress-inducible protein 2

1 Images

Supplier Data

Western blot - Anti-TTC21A/STI2 antibody [EPR17437] (AB201229)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TTC21A/STI2 antibody [EPR17437] (ab201229) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Lane 4:

A549 (Human lung carcinoma) whole cell lysate

Lane 5:

Human fetal brain lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Lane 7:

Human fetal kidney lysate at 10 µg

Lane 8:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 151 kDa,83 kDa

Observed band size: 150 kDa

false

Exposure time: 3min

Carrier free

Anti-TTC21A/STI2 antibody [EPR17437] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17437

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC21A also known as STI2 is a protein with a mass of approximately 145 kDa. It functions as a component of the intraflagellar transport system which is essential for the maintenance and function of cilia. TTC21A is expressed in various tissues with significant expression in the kidney and lungs. This protein plays a role in stabilizing microtubules and aiding the movement of protein complexes along the cilia's axoneme.

Biological function summary

TTC21A plays an important role in ciliogenesis and the maintenance of ciliary structure. It is part of the intraflagellar transport complex which includes proteins such as IFT-A and IFT-B. This complex is essential for the bidirectional movement of molecules along the cilia which is important for their sensory and signaling functions. By interacting with other IFT proteins TTC21A ensures proper assembly and disassembly of ciliary components.

Pathways

TTC21A is involved in the Hedgehog signaling pathway which is important for embryonic development and tissue regeneration. During this process TTC21A interacts with proteins such as Smoothened (SMO) and Patched (PTCH) to regulate signal transmission. Moreover TTC21A is connected to the Wnt signaling pathway which has critical roles in cell proliferation and differentiation. The interaction with Dishevelled (DVL) proteins within this pathway highlights its importance in maintaining cellular communication.

Defects in TTC21A are associated with nephronophthisis and Jeune syndrome. Nephronophthisis a kidney disorder involves abnormal ciliary function where TTC21A's interaction with nephrocystin proteins is compromised. In Jeune syndrome a skeletal dysplasia TTC21A's role in ciliary function impacts the development of bone and cartilage. These diseases highlight TTC21A's importance in developmental processes and highlight the critical nature of its interactions within cellular pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Intraflagellar transport (IFT)-associated protein required for spermatogenesis (PubMed : 30929735). Required for sperm flagellar formation and intraflagellar transport (PubMed : 30929735).

See full target information TTC21A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Circulation 146:1674-1693 PubMed36321451

2022

Pathogenesis of Cardiomyopathy Caused by Variants in , an Essential Pseudokinase in the Cardiomyocyte Nucleus and Sarcomere.

Applications

Unspecified application

Species

Unspecified reactive species

Radhika Agarwal,Hiroko Wakimoto,Joao A Paulo,Qi Zhang,Daniel Reichart,Christopher Toepfer,Arun Sharma,Angela C Tai,Mingyue Lun,Joshua Gorham,Steven R DePalma,Steven P Gygi,J G Seidman,Christine E Seidman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TTC21A/STI2 antibody [EPR17437]

Western blot - Anti-TTC21A/STI2 antibody [EPR17437] (AB201229)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise