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AB251339

Anti-TTC21A/STI2 antibody [EPR17437] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TTC21A/STI2 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

STI2, TTC21A, Tetratricopeptide repeat protein 21A, TPR repeat protein 21A, Stress-inducible protein 2

1 Images
Western blot - Anti-TTC21A/STI2 antibody [EPR17437] - BSA and Azide free (AB251339)
  • WB

Supplier Data

Western blot - Anti-TTC21A/STI2 antibody [EPR17437] - BSA and Azide free (AB251339)

This data was developed using ab201229, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TTC21A/STI2 antibody [EPR17437] (<a href='/en-us/products/primary-antibodies/ttc21a-sti2-antibody-epr17437-ab201229'>ab201229</a>) at 1/10000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Human cerebellum lysate at 10 µg

Lane 3:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 10 µg

Lane 4:

A549 (Human lung carcinoma) whole cell lysate

Lane 5:

Human fetal brain lysate at 10 µg

Lane 6:

Human fetal heart lysate at 10 µg

Lane 7:

Human fetal kidney lysate at 10 µg

Lane 8:

Human fetal spleen lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 151 kDa,83 kDa

Observed band size: 150 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17437

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251339 is the carrier-free version of ab201229.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC21A also known as STI2 is a protein with a mass of approximately 145 kDa. It functions as a component of the intraflagellar transport system which is essential for the maintenance and function of cilia. TTC21A is expressed in various tissues with significant expression in the kidney and lungs. This protein plays a role in stabilizing microtubules and aiding the movement of protein complexes along the cilia's axoneme.
Biological function summary

TTC21A plays an important role in ciliogenesis and the maintenance of ciliary structure. It is part of the intraflagellar transport complex which includes proteins such as IFT-A and IFT-B. This complex is essential for the bidirectional movement of molecules along the cilia which is important for their sensory and signaling functions. By interacting with other IFT proteins TTC21A ensures proper assembly and disassembly of ciliary components.

Pathways

TTC21A is involved in the Hedgehog signaling pathway which is important for embryonic development and tissue regeneration. During this process TTC21A interacts with proteins such as Smoothened (SMO) and Patched (PTCH) to regulate signal transmission. Moreover TTC21A is connected to the Wnt signaling pathway which has critical roles in cell proliferation and differentiation. The interaction with Dishevelled (DVL) proteins within this pathway highlights its importance in maintaining cellular communication.

Defects in TTC21A are associated with nephronophthisis and Jeune syndrome. Nephronophthisis a kidney disorder involves abnormal ciliary function where TTC21A's interaction with nephrocystin proteins is compromised. In Jeune syndrome a skeletal dysplasia TTC21A's role in ciliary function impacts the development of bone and cartilage. These diseases highlight TTC21A's importance in developmental processes and highlight the critical nature of its interactions within cellular pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Intraflagellar transport (IFT)-associated protein required for spermatogenesis (PubMed : 30929735). Required for sperm flagellar formation and intraflagellar transport (PubMed : 30929735).
See full target information TTC21A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 22:800 PubMed39731111

2024

Role of PCBP2 in regulating nanovesicles loaded with curcumin to mitigate neuroferroptosis in neural damage caused by heat stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Guo,Yizhan Wu,Guangjun Wang,Jiangwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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