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AB181162

Anti-TTC27 antibody [EPR13653]

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(1 Publication)

Rabbit Recombinant Monoclonal TTC27 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Tetratricopeptide repeat protein 27, TPR repeat protein 27, TTC27

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TTC27 antibody [EPR13653] (AB181162)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC27 antibody [EPR13653] (AB181162)

Immunocytochemical staining of HeLa cells fixed in Acetone at -20℃, labeling TTC27 with ab181162 at 1/100 dilution and Goat anti rabbit IgG (Alexa Fluor® 555) at 1/200 dilution (red) and counterstained with DAPI (blue)

Western blot - Anti-TTC27 antibody [EPR13653] (AB181162)
  • WB

Supplier Data

Western blot - Anti-TTC27 antibody [EPR13653] (AB181162)

All lanes:

Western blot - Anti-TTC27 antibody [EPR13653] (ab181162) at 1/5000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

A673 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 97 kDa

false

  • Carrier free

    Anti-TTC27 antibody [EPR13653] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13653

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC27 also known as Tetratricopeptide Repeat Protein 27 is a protein characterized by its tetratricopeptide repeat (TPR) domains. The molecular mass of TTC27 is approximately 143 kDa. It is expressed in various tissues with particularly high levels in the brain liver and testes. These TPR domains suggest that TTC27 may be involved in protein-protein interactions functioning as a scaffold for the assembly of multiprotein complexes.
Biological function summary

TTC27 plays important roles in cellular processes. It functions as a part of larger multiprotein complexes which indicates its role in maintaining cellular homeostasis and possibly influencing signaling pathways. The protein's distribution in the brain suggests its involvement in neurological functions though detailed mechanisms remain under investigation. TTC27's interaction with other proteins points towards its potential role in regulating cellular signaling and response.

Pathways

TTC27's involvement in specific signaling pathways highlights its importance in cellular communication. Recent studies have linked TTC27 to the MAPK/ERK pathway a critical pathway for cell proliferation and differentiation. It interacts with proteins such as MEK and ERK facilitating the transmission of extracellular signals to the nucleus. Additionally TTC27 may participate in the NOD-like receptor signaling pathway further influencing immune responses and inflammation processes.

TTC27's function and expression profile suggest potential links to certain conditions. Research indicates a connection between TTC27 and certain cancers where alterations in signaling pathways like MAPK/ERK could contribute to tumorigenesis. The correlation with proteins like ERK involved in cell cycle regulation highlights its role in cancer development. Furthermore TTC27's significant expression in the brain suggests possible involvement in neurological disorders drawing interest in its relationship with proteins implicated in neurodegenerative diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information TTC27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:9 PubMed36599853

2023

PQBP5/NOL10 maintains and anchors the nucleolus under physiological and osmotic stress conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaocen Jin,Hikari Tanaka,Meihua Jin,Kyota Fujita,Hidenori Homma,Maiko Inotsume,Huang Yong,Kenichi Umeda,Noriyuki Kodera,Toshio Ando,Hitoshi Okazawa
View all publications
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Product promise

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