AB197076

Anti-TTC28/TPRBK antibody - C-terminal

Name: Anti-TTC28/TPRBK antibody - C-terminal (ab197076) | Abcam
Brand: Abcam Limited
SKU: AB197076

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(2 Publications)

Rabbit Polyclonal TTC28/TPRBK antibody. C-terminal. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human TTC28 aa 2450 to C-terminus.

View Alternative Names

KIAA1043, TPRBK, TTC28, Tetratricopeptide repeat protein 28, TPR repeat protein 28, TPR repeat-containing big gene cloned at Keio

2 Images

Supplier Data

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Detection of TTC28/TPRBK in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab197076 at 6 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (ab197076)

Predicted band size: 271 kDa

false

Supplier Data

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Lysates prepared wtih NETN lysis buffer.

All lanes:

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (ab197076) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Predicted band size: 271 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TTC28 aa 2450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q96AY4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab197076 was affinity purified using an epitope specific to TTC28/TPRBK immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The TTC28 also known as TPRBK is a protein with a molecular weight of approximately 230 kDa. This protein consists of tetratricopeptide repeat domains that are important for protein-protein interactions. TTC28 exhibits a broad expression pattern in human tissues but shows higher expression levels in the brain testis and certain cancerous tissues. Its widespread expression suggests a fundamental role across various biological systems.

Biological function summary

TTC28/TPRBK may influence cellular processes including cell cycle regulation and protein folding. This protein interacts with other proteins as part of a multiprotein complex including components of the cytoskeleton and cell cycle regulators. Its participation in these important processes suggests that TTC28 plays a role in maintaining cellular integrity and function.

Pathways

When considering the cellular mechanism TTC28/TPRBK participates in the cell cycle regulation pathway and possibly impacts cell division. It shows interaction with proteins like cyclins and cyclin-dependent kinases which are central to the progression of the cell cycle. These associations indicate that TTC28's function in pathways reflects its functional importance in regulating proper cellular division and growth.

Researchers associate TTC28/TPRBK with specific types of cancer such as glioblastoma and breast cancer. Aberrant expression or dysfunction of TTC28 may disrupt normal cell cycle progression leading to uncontrolled cell proliferation. Its interaction with oncoproteins such as Cyclin D1 further corroborates its potential role in oncogenesis. Understanding TTC28's involvement in these diseases can contribute to developing targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

During mitosis, may be involved in the condensation of spindle midzone microtubules, leading to the formation of midbody.

See full target information TTC28

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 121:e2409447121 PubMed39630868

2024

The essential role of TTC28 in maintaining chromosomal stability via HSPA8 chaperone-mediated autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ge Zhang,Meiyi Xiang,Liankun Gu,Jing Zhou,Baozhen Zhang,Wei Tian,Dajun Deng

Neuropsychopharmacology : official publication of 45:1884-1895 PubMed32610340

2020

Activation of tyrosine phosphatase PTP1B in pyramidal neurons impairs endocannabinoid signaling by tyrosine receptor kinase trkB and causes schizophrenia-like behaviors in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaohong Qin,Li Zhang,Shelly A Cruz,Alexandre F R Stewart,Hsiao-Huei Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-TTC28/TPRBK antibody - C-terminal

Immunoprecipitation - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Western blot - Anti-TTC28/TPRBK antibody - C-terminal (AB197076)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise