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AB181195

Anti-TTC4 antibody [EPR13381-76]

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(3 Publications)

Rabbit Recombinant Monoclonal TTC4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

My044, TTC4, Tetratricopeptide repeat protein 4, TPR repeat protein 4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] (AB181195)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TTC4 antibody [EPR13381-76] (AB181195)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling TTC4 with ab181195 at 1/100 dilution. Secondary antibody : anti rabbit IgG (HRP) prediluted. Counter stain : Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381-76] (AB181195)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TTC4 antibody [EPR13381-76] (AB181195)

Immunofluorescent analysis of 4% paraformaldehyde fixed MCF7 cells labeling TTC4 with ab181195 at 1/250 dilution. Secondary antibody : Goat anti rabbit IgG (Alexa Fluor® 488) at a 1/200 dilution. Conterstained with Dapi (blue).

Western blot - Anti-TTC4 antibody [EPR13381-76] (AB181195)
  • WB

Supplier Data

Western blot - Anti-TTC4 antibody [EPR13381-76] (AB181195)

All lanes:

Western blot - Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/5000 dilution

All lanes:

MCF7 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

Western blot - Anti-TTC4 antibody [EPR13381-76] (AB181195)
  • WB

Supplier Data

Western blot - Anti-TTC4 antibody [EPR13381-76] (AB181195)

All lanes:

Western blot - Anti-TTC4 antibody [EPR13381-76] (ab181195) at 1/20000 dilution

Lane 1:

293T cell lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

U-87 MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 45 kDa

false

  • Carrier free

    Anti-TTC4 antibody [EPR13381-76] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13381-76

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC4 also known as Tetratricopeptide Repeat Protein 4 has a molecular mass of approximately 44 kDa. It functions as a molecular chaperone assisting in the folding and stability of other proteins. TTC4 contains tetratricopeptide repeat (TPR) motifs which play a role in protein-protein interactions. These motifs enable TTC4 to interact with various cellular proteins. It is expressed widely in human tissues with higher levels observed in the heart brain and testis.
Biological function summary

The tetratricopeptide repeats in TTC4 facilitate its role as a part of multiprotein complexes. This protein takes part in regulating important cellular processes like cell cycle control and stress response. TTC4 assists in stabilizing proteins during cellular stress ensuring proper cellular function under adverse conditions. It may also interact with heat shock proteins (HSPs) contributing to protein homeostasis in cells.

Pathways

This protein is involved in important cellular processes including the signal transduction pathways and protein quality control pathways. TTC4 is notably involved in the chaperone-mediated autophagy pathway where it regulates the turnover and recycling of damaged proteins. It relates to proteins such as Hsp70 which are central to cellular stress response mechanisms. This relationship plays an important role in maintaining protein stability and cellular function.

TTC4's role in maintaining cellular protein stability links it to conditions caused by protein misfolding. Misregulation in its function may contribute to neurodegenerative diseases such as Alzheimer's disease where excessive protein aggregation occurs. Additionally TTC4 interacts with proteins like Tau which are important in the pathogenesis of Alzheimer's. Aberrations in TTC4 might also influence cancer progression due to its involvement in cell cycle regulation linking it to proteins such as p53 known for its tumor suppressor role.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May act as a co-chaperone for HSP90AB1 (PubMed : 18320024). Promotes Sendai virus (SeV)-induced host cell innate immune responses (PubMed : 29251827).
See full target information TTC4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

American journal of cancer research 13:5122-5137 PubMed38058818

2023

Methylation of TTC4 interaction with HSP70 inhibits pyroptosis in macrophages of sepsis-induced lung injury by NLRP3 inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Chen,Jiwen Liu,Kening Sun,Liru Wang,Xia Li,Xiang Li,Wei Jia,Xiaoyan Yuan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:12932-12945 PubMed33000523

2020

Promoting TTC4 and HSP70 interaction and translocation of annexin A7 to lysosome inhibits apoptosis in vascular endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying He,Zhaomin Lin,Junya Ning,Na Li,Xiaoling Cui,Baoxiang Zhao,Fanzhen Hong,Junying Miao

The FEBS journal 286:4937-4950 PubMed31408583

2019

MROH7-TTC4 read-through lncRNA suppresses vascular endothelial cell apoptosis and is upregulated by inhibition of ANXA7 GTPase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoying He,Xuan Zhao,Le Su,Baoxiang Zhao,Junying Miao
View all publications
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Product promise

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