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AB251172

Anti-TTC7B antibody [EPR15995] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal TTC7B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TTC7L1, TTC7B, Tetratricopeptide repeat protein 7B, TPR repeat protein 7B, Tetratricopeptide repeat protein 7-like-1, TPR repeat protein 7-like-1

2 Images
Immunoprecipitation - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172)
  • IP

Supplier Data

Immunoprecipitation - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172)

This data was developed using ab193361, the same antibody clone in a different buffer formulation.

Western blot analysis of TTC7B immunoprecipitated from K562 cell lysate using ab193361 at 1/60 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TTC7B antibody [EPR15995] (<a href='/en-us/products/primary-antibodies/ttc7b-antibody-epr15995-ab193361'>ab193361</a>)

Predicted band size: 94 kDa

false

Western blot - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172)
  • WB

Supplier Data

Western blot - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172)

This data was developed using ab193361, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TTC7B antibody [EPR15995] (<a href='/en-us/products/primary-antibodies/ttc7b-antibody-epr15995-ab193361'>ab193361</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Human fetal brain lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 94 kDa

Observed band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15995

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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  • 4
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251172 is the carrier-free version of ab193361.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TTC7B also known as Tetratricopeptide Repeat Domain 7B is a protein with a mass of approximately 114 kDa. Mechanically it contains several tetratricopeptide repeat (TPR) motifs that facilitate protein-protein interactions. This protein is expressed in many tissues with notable levels in the liver and kidney. Its role is to help with various cellular processes likely through binding with other proteins to form functional complexes.
Biological function summary

TTC7B contributes to cellular functions through its involvement in the phosphatidylinositol-4 kinase pathway. This pathway is important for the regulation of cell signaling and membrane trafficking. TTC7B is part of the GOLPH3/TTC7B complex which maintains proper Golgi function and morphology an essential aspect for vesicular transport and signal transduction.

Pathways

The phosphatidylinositol signaling and inositol phosphate metabolism pathways require TTC7B's participation. These pathways are critical for various cellular processes including cell growth survival and communication. TTC7B interfaces with proteins such as PI4KA and FAM126A which are important for phosphatidylinositol 4-phosphate synthesis impacting overall pathway functionality.

Improper TTC7B function can associate with Golgi function-related disorders. Conditions such as Lenz-Majewski hyperostotic dwarfism have connections to the dysregulation of pathways involving TTC7B. In these contexts proteins like PI4KA also interact in the disease mechanism illustrating TTC7B's significance in maintaining cellular and physiological balance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, plays a central role in bridging PI4KA to EFR3B and HYCC1, via direct interactions (PubMed : 26571211).
See full target information TTC7B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:4027-4050 PubMed40612676

2025

Exogenous SPD inhibits trastuzumab-mediated cardiomyocyte pyroptosis through SIRT3-regulated mitochondrial quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Yu,Yan Yang,Tianzuo Chen,Qianbing Wang,Zitong Wang,Xi Gao,Qianxue Wang,Jinxiang Guo,Yuqin Wang,Yajie Zhao,Shilin Wang,Wei Lu,Xing Luo,Tielei Gao,Jiayuan Kou,Hong Li,Liming Yang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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