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Rabbit Recombinant Monoclonal TTC7B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

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Images

Western blot - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172), expandable thumbnail
  • Immunoprecipitation - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (AB251172), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Component of a complex required to localize phosphatidylinositol 4-kinase (PI4K) to the plasma membrane. The complex acts as a regulator of phosphatidylinositol 4-phosphate (PtdIns(4)P) synthesis. In the complex, plays a central role in bridging PI4KA to EFR3B and HYCC1, via direct interactions (PubMed:26571211).

Alternative names

TTC7L1, TTC7B, Tetratricopeptide repeat protein 7B, TPR repeat protein 7B, Tetratricopeptide repeat protein 7-like-1, TPR repeat protein 7-like-1

Recommended products

Rabbit Recombinant Monoclonal TTC7B antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR15995
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab251172 is the carrier-free version of Anti-TTC7B antibody [EPR15995] ab193361.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TTC7B also known as Tetratricopeptide Repeat Domain 7B is a protein with a mass of approximately 114 kDa. Mechanically it contains several tetratricopeptide repeat (TPR) motifs that facilitate protein-protein interactions. This protein is expressed in many tissues with notable levels in the liver and kidney. Its role is to help with various cellular processes likely through binding with other proteins to form functional complexes.

Biological function summary

TTC7B contributes to cellular functions through its involvement in the phosphatidylinositol-4 kinase pathway. This pathway is important for the regulation of cell signaling and membrane trafficking. TTC7B is part of the GOLPH3/TTC7B complex which maintains proper Golgi function and morphology an essential aspect for vesicular transport and signal transduction.

Pathways

The phosphatidylinositol signaling and inositol phosphate metabolism pathways require TTC7B's participation. These pathways are critical for various cellular processes including cell growth survival and communication. TTC7B interfaces with proteins such as PI4KA and FAM126A which are important for phosphatidylinositol 4-phosphate synthesis impacting overall pathway functionality.

Associated diseases and disorders

Improper TTC7B function can associate with Golgi function-related disorders. Conditions such as Lenz-Majewski hyperostotic dwarfism have connections to the dysregulation of pathways involving TTC7B. In these contexts proteins like PI4KA also interact in the disease mechanism illustrating TTC7B's significance in maintaining cellular and physiological balance.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (ab251172), expandable thumbnail

    Western blot - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (ab251172)

    This data was developed using Anti-TTC7B antibody [EPR15995] ab193361, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-TTC7B antibody [EPR15995] (Anti-TTC7B antibody [EPR15995] ab193361) at 1/1000 dilution

    Lane 1: K562 cell lysate at 20 µg

    Lane 2: Human fetal brain lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 94 kDa

    Observed band size: 94 kDa

  • Immunoprecipitation - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (ab251172), expandable thumbnail

    Immunoprecipitation - Anti-TTC7B antibody [EPR15995] - BSA and Azide free (ab251172)

    This data was developed using Anti-TTC7B antibody [EPR15995] ab193361, the same antibody clone in a different buffer formulation.Western blot analysis of TTC7B immunoprecipitated from K562 cell lysate using Anti-TTC7B antibody [EPR15995] ab193361 at 1/60 dilution.Secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.Blocking/Dilution buffer: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-TTC7B antibody [EPR15995] (Anti-TTC7B antibody [EPR15995] ab193361)

    Predicted band size: 94 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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